Refolding and purification of recombinant OsNifU1A domain II that was expressed by Escherichia coli

Protein Expression and Purification

Volumn 43, Issue 2, 2005, Pages 149-156

  Katoh, Shizue ^a   Murata, Katsuyoshi ^a   Kubota, Yoshiki ^b   Kumeta, Hiroyuki ^c   Ogura, Kenji ^c   Inagaki, Fuyuhiko ^c   Asayama, Munehiko ^b   Katoh, Etsuko ^a  

^a NATIONAL INSTITUTE OF AGROBIOLOGICAL SCIENCES   (Japan)

^b IBARAKI UNIVERSITY   (Japan)

^c HOKKAIDO UNIVERSITY   (Japan)

Author keywords

CnfU; Iron sulfur protein; NifU; Oryza sativa; Refolding

Indexed keywords

CYANOBACTERIA; ESCHERICHIA COLI; ORYZA SATIVA;

HYBRID PROTEIN; IRON SULFUR PROTEIN; VEGETABLE PROTEIN;

ARTICLE; CHEMISTRY; ENZYMOLOGY; ESCHERICHIA COLI; GENETICS; ISOLATION AND PURIFICATION; NITROGEN FIXATION; NUCLEAR MAGNETIC RESONANCE; PROTEIN FOLDING; PROTEIN TERTIARY STRUCTURE; RICE;

ESCHERICHIA COLI; IRON-SULFUR PROTEINS; NITROGEN FIXATION; NUCLEAR MAGNETIC RESONANCE, BIOMOLECULAR; ORYZA SATIVA; PLANT PROTEINS; PROTEIN FOLDING; PROTEIN STRUCTURE, TERTIARY; RECOMBINANT FUSION PROTEINS;

EID: 32944466765     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2005.04.019     Document Type: Article

References (35)

1. R. Cammack Advances in inorganic chemistry: Iron-sulfur proteins 1992 Academic Press San Diego
2. M.K. Johnson Iron-sulfur proteins: new roles for old cluster Curr. Opin. Chem. Biol. 2 1989 173 181
3. L. Zheng, R.H. White, V.L. Cash, R.F. Jack, and D.R. Dean Cysteine desulfurase activity indicates a role for NIFS in metallocluster biosynthesis Proc. Natl. Acad. Sci. USA 90 1993 2754 2758
4. L. Zheng, and D.R. Dean Catalytic formation of a nitrogenase iron-sulfur cluster J. Biol. Chem. 269 1994 18723 18726
5. L. Zheng, V.L. Cash, D.H. Flint, and D.R. Dean Assembly of iron-sulfur clusters identification of an iscSUA-hscBA-fdx gene cluster from Azotobacter vinelandii J. Biol. Chem. 273 1998 13264 13272
6. M. Nakamura, K. Saeki, and Y. Takahashi Hyperproduction of recombinant ferredoxins in Escherichia coli by coexpression of the ORF1-ORF2-iscS-iscU-iscA- hscB-hscA-fdx-ORF3 gene cluster J. Biochem (Tokyo) 126 1999 10 18
7. Y. Takahashi, and M. Nakamura Functional assignment of the ORF2-iscS-iscU-iscA-hscB-hscA-fdx-ORF3 gene cluster involved in the assembly of Fe-S clusters in Escherichia coli J. Biochem. (Tokyo) 126 1999 917 926
8. Y. Nakai, Y. Yoshida, H. Hayashi, and H. Kagamiyama cDNA cloning and characterization of mouse NifS-like protein, m-Nfs1: mitochondrial localization of eukaryotic NifS-like proteins FEBS Lett. 433 1998 143 148
9. J. Strain, C.R. Lorenz, J. Bode, S. Garland, G.A. Smolen, D.T. Ta, L.E. Vickery, and V.C. Culotta Suppressors of superoxide dismutase (SOD1) deficiency in Saccharomyces cerevisiae Identification of proteins predicted to mediate iron-sulfur cluster assembly J. Biol. Chem. 273 1998 31138 31144
10. G. Kispal, P. Csere, C. Prohl, and R. Lill The mitochondrial proteins Atm1p and Nfs1p are essential for biogenesis of cytosolic Fe/S proteins EMBO J. 18 1999 3981 3989
11. J. Li, M. Kogan, S.A.B. Knight, D. Pain, and A. Dancis Yeast mitochondrial protein, Nfs1p, coordinately regulates iron-sulfur cluster proteins, cellular iron uptake, and iron distribution J. Biol. Chem. 274 1999 33025 33034
12. B. Schike, C. Voisine, H. Beinert, and E. Craig Evidence for a conserved system for iron metabolism in the mitochondria of Saccharomyces cerevisiae Proc. Natl. Acad. Sci. USA 96 1999 10206 10211
13. S.A. Garl, K. Hoff, L.E. Vickery, and V.C. Culotta Sccharomyces cerevisiae ISU1 and ISU2: members of a well-conserved gene family for iron-sulfur cluster assembly J. Mol. Biol. 294 1999 897 907
14. R. Lill, K. Diekert, A. Kaut, H. Lange, W. Pelzer, C. Prohl, and G. Kispal The essential role of mitochondria in the biogenesis of cellular iron-sulfur proteins Biol. Chem. 380 1999 1157 1166
15. E.A. Craig, C. Voisine, and B. Schilke Mitochondrial iron metabolism in the yeast Saccharomyces cerevisiae Biol. Chem. 380 1999 1167 1173
16. J. Frazzon, J.R. Fick, and D.R. Dean Biosynthesis of iron-sulfur clusters is a complex and highly conserved process Biochem. Soc. Trans. 30 2002 680 685
17. U. Muhlenhoff, and R. Lill Biosynthesis of iron-sulfur proteins in eukaryotes: A novel task of mitochondria that is inherited from bacteria Biochim. Biophys. Acta 1459 2000 370 382
18. J. Frazzon, and D.R. Dean Formation of iron-sulfur clusters in bacteria: An emerging field in bioinorganic chemistry Curr. Opin. Chem. Biol. 7 2003 166 173
19. H.D. Urbina, J.J. Silberg, K.G. Hoff, and L.E. Vickery Transfer of sulfur from IscS to IscU during Fe/S cluster assembly J. Biol. Chem. 276 2001 44521 44526
20. J.N. Agar, P. Yuvaniyama, R.F. Jack, V.L. Cash, A.F. Smith, D.R. Dean, and M.K. Johnson Modular organization and identification of a mononuclear iron-binding site within the NifU protein J. Biol. Inorg. Chem. 5 2000 167 177
21. K. Nishio, and M. Nakai Transfer of iron-sulfur cluster from NifU to apoferredoxin J. Biol. Chem. 275 2000 22615 22618
22. P. Yuvaniyama, J.N. Agar, V.L. Cash, M.K. Johnson, and D.R. Dean NifS-directed assembly of a transient [2Fe-2S] cluster within the NifU protein Proc. Natl. Acad. Sci. USA 97 2000 599 604
23. C. Krebs, J.N. Agar, A.D. Smith, J. Frazzon, D.R. Dean, B.H. Huynh, and M.K. Johnson IscA, an alternate scaffold for Fe-S cluster biosynthesis Biochemistry 40 2001 14069 14080
24. S. Ollagnier-de-Choudens, T. Mattioli, Y. Takahashi, and M. Frontecave Iron-sulfur cluster assembly: characterization of IscA and evidence for a specific and functional complex with ferredoxin J. Biol. Chem. 276 2001 22604 22607
25. C. Ouzounis, P. Bork, and C. Sander The modular structure of NifU proteins Trends Biochem. Sci. 19 1994 199 200
26. W. Fu, R.F. Jack, T.V. Morgan, D.R. Dean, and M.K. Johnson NifU gene product from Azotobacter vinelandii is a homodimer that contains two identical [2Fe-2S] clusters Biochemistry 33 1994 13455 13463
27. T. Yabe, K. Morimoto, S. Kikuchi, K. Nishio, I. Terashima, and M. Nakai The Arabidopsis chloroplastic NifU-like protein CnfU, which can act as an iron-sulfur cluster scaffold protein, is required for biogenesis of ferredoxin and photosystem 1 Plant Cell 16 2004 993 1007
28. Y. Tsunoda, N. Sakai, K. Kikuchi, S. Katoh, K. Akagi, J.O. Miura, Y. Tashiro, K. Murata, N. Shibuya, E. Katoh, Improving expression and solubility of rice proteins produced as fusion proteins in Escherichia coli, Protein Pur. Exp. (in press).
29. F. Delaglio, S. Grzesiek, G.W. Vuister, G. Zhu, J. Pfeifer, and A. Bax NMRPipe: a multidimensional spectral processing system based on UNIX pipes J. Biol. NMR 6 1995 277 293
30. A. Bax, and S. Subramanian Sensitivity enhanced two-dimensional heteronuclear shift correlation NMR spectroscopy J. Magn. Reson. 67 1986 565 569
31. 15N NMR study of gramicidin S in water and organic solvents J. Am. Chem. Soc. 106 1984 1934 1941
32. E.R. LaVallie, E.A. DiBlasio-Smith, L.A. Collins-Racie, Z. Lu, and J.M. McCoy Thioredoxin and related proteins as multifunctional fusion tags for soluble expression in E. coli Methods Mol. Biol. 205 2003 119 140
33. R. Ishima, and D.A. Torchia Protein dynamics from NMR Nat. Struct. Biol. 7 2000 740 743
34. L. Moise, A. Piserchio, V.J. Basus, and E. Hawrot NMR structural analysis of alpha-bungarotoxin and its complex with the principal alpha-neurotoxin- binding sequence on the alpha 7 subunit of a neuronal nicotinic acetylcholine receptor J. Biol. Chem. 277 2002 12406 12417
35. H. Lilie, E. Schwarz, and R. Roudolph Advances in refolding of proteins produced in E. coli Curr. Opin. Biotechnol. 9 1998 497 501