Sequence analysis of partial toxR gene from Philippine Vibrio isolates and design of toxR-targeted primers for detection

Journal of General and Applied Microbiology

Volumn 51, Issue 6, 2006, Pages 343-351

  Rañoa, Diana Rose E ^a   Hedreyda, Cynthia T ^a  

^a UNIVERSITY OF THE PHILIPPINES   (Philippines)

Author keywords

PCR; toxR; Vibrio campbellii; Vibrio harveyi; Vibriosis

Indexed keywords

PRIMER DNA; TRANSCRIPTION FACTOR; TRANSCRIPTION FACTOR TOXR; UNCLASSIFIED DRUG;

ARTICLE; BACTERIUM DETECTION; BACTERIUM ISOLATE; CONTROLLED STUDY; DNA SEQUENCE; DNA TEMPLATE; FOOD INDUSTRY; GENE AMPLIFICATION; GENE TARGETING; GENETIC ENGINEERING; GENETIC VARIABILITY; MORTALITY; NONHUMAN; NUCLEOTIDE SEQUENCE; PENAEIDAE; PENAEUS MONODON; PHILIPPINES; POLYMERASE CHAIN REACTION; SEQUENCE HOMOLOGY; SHRIMP; STRAIN IDENTIFICATION; TYPE STRAIN; VIBRIO; VIBRIO CAMPBELLII; VIBRIO HARVEYI; VIBRIONACEAE;

AMINO ACID SEQUENCE; BACTERIAL PROTEINS; BACTERIAL TYPING TECHNIQUES; BASE SEQUENCE; DNA PRIMERS; DNA, BACTERIAL; DNA-BINDING PROTEINS; MOLECULAR SEQUENCE DATA; PHILIPPINES; POLYMERASE CHAIN REACTION; SEAFOOD; SEQUENCE ANALYSIS; TRANSCRIPTION FACTORS; VIBRIO;

BACTERIA (MICROORGANISMS); DECAPODA (CRUSTACEA); PENAEIDAE; PENAEUS MONODON; VIBRIO; VIBRIO CAMPBELLII; VIBRIO HARVEYI; VIBRIONACEAE;

EID: 32944462426     PISSN: 00221260     EISSN: None     Source Type: Journal    
DOI: 10.2323/jgam.51.343     Document Type: Article

References (16)

1. Conejero, M. J. U. and Hedreyda, C. T. (2003) Isolation of partial toxR gene of Vibrio harveyi and design of toxR-targeted PCR primers for species detection. J. Appl. Microbiol., 95, 602-611.
2. Cortado, H. H., San Luis, B. B., de la Peña, L. D., Monsalud, R. G., and Hedreyda, C. T. (2004) Local Vibrio isolates exhibit molecular characteristics distinct from reference V. harveyi and V. campbellii strains. Sci. Diliman, 16, 23-30.
3. de la Peña, L. D., Lavilla-Pitogo, C. R., and Paner, M. G. (2001) Luminescent Vibrios associated with mortality in pond-cultured shrimp Penaeus monodon in the Philippines: Species composition. Fish Pathol., 36, 133-138.
4. Karunasagar, I., Pai, R., Malathi, G. R., and Karunasagar, I. (1994) Mass mortality of Penaeus monodon larvae due to antibiotic-resistant Vibrio harveyi infection. Aquaculture, 195, 193-204.
5. Kim, Y. B., Okuda, J., Matsumoto, C., Takahashi, N., Hashimoto, S., and Nishibuchi, M. (1999) Identification of Vibrio parahaemolyticus at the species level by PCR targeted to the toxR gene. J. Clin. Microbiol., 37, 1173-1177.
6. Kita-Tsukamoto, K., Oyaizu, H., Nanba, K., and Shimidu, U. (1993) Phylogenetic relationships of marine bacteria, mainly members of the family Vibrionaceae, determined on the basis of 16S rRNA sequences. Int. J. Syst. Bacteriol., 43, 8-19.
7. Lavilla-Pitogo, C. R., Baticados, M. C. L., Cruz-Lacierda, E. R., and de la Peña, L. D. (1990) Occurrence of luminous bacterial disease of Penaeus monodon larvae in the Philippines. Aquaculture, 91, 1-13.
8. Lavilla-Pitogo, C. R., Leaño, E. M., and Paner, M. G. (1998) Mortalities of pond-cultured juvenile shrimp, Penaeus monodon, associated with dominance of luminescent vibrios in the rearing environment. Aquaculture, 164, 337-349.
9. Lee, S. E., Shin, S. H., Kim, S. Y., Kim, Y. R., Shin, D. H., Chung, S. S., Lee, Z. H., Lee, J. Y., Jeong, K. C., Choi, S. H., and Rhee, J. H. (2000) Vibrio vulnificus has the transmembrane transcription activator ToxRS stimulating the expression of the hemolysin gene vvhA. J. Bacteriol., 182, 3405-3415.
10. Lin, Z., Kumagai, K., Baba, K., Mekalanos, J. J., and Nishibuchi, M. (1993) Vibrio parahaemolyticus has a homolog of the Vibrio cholerae toxRS operon that mediates environmentally induced regulation of the thermostable direct hemolysin. J. Bacteriol., 175, 3844-3855.
11. Osorio, C. R. and Klose, K. E. (2000) A region of the transmembrane regulatory protein ToxR that tethers the transcription activation domain to the cytoplasmic membrane displays wide divergence among Vibrio species. J. Bacteriol., 182, 526-528.
12. Reich, K. A. and Schoolnik, G. K. (1994) The light organ symbiont Vibrio fischeri possesses a homolog of the Vibrio cholerae transmembrane transcription activator ToxR. J. Bacteriol., 176, 3085-3088.
13. San Luis, B. B. and Hedreyda, C. T. (2005) Partial gyrB, toxR, and hemolysin gene sequence from two strains of Vibrio campbellii for phylogenetic analysis and species detection. Proceedings of the 34th Annual Philippine Society for Microbiology Convention and Scientific Meeting. Waterfront Insular Hotel, Davao City, Philippines.
14. Vuddhakul, V., Nakai, T., Matsumoto, C., Oh, T., Nishino, T., Chen, C.-H., Nishibuchi. M., and Okuda, J. (2000) Analysis of gyrB and toxR gene sequences of Vibrio hollisae and development of gyrB and toxR-targeted PCR methods for isolation of V. hollisae from the environment and its identification. Appl. Environ. Microbiol., 66, 3506-3514.
15. Welch, T. J. and Bartlett, D. H. (1998) Identification of a regulatory protein required for pressure-responsive gene regulation in the deep-sea bacterium Photobacterium species strain SS9. J. Bacteriol., 178, 5027-5031.
16. West, P. A., Brayton, P. R., Bryant, T. N., and Colwell, R. R. (1986) Numerical taxonomy of species of Vibrio isolated from aquatic environments. Int. J. Syst. Bacteriol., 36, 531-543.