The promoter competition assay (PCA): A new approach to identify motifs involved in the transcriptional activity of reporter genes

Frontiers in Bioscience

Volumn 11, Issue 2 P.1199-1590, 2006, Pages 1577-1584

  Hubé, Florent ^a   Myal, Yvonne ^a   Leygue, Etienne ^a  

^a UNIVERSITY OF MANITOBA   (Canada)

Author keywords

Luciferase Reporter Gene; Promoter Competition Assay; Small Breast Epithelial Mucin; Transcription Factor Binding Site

Indexed keywords

EID: 32844457619     PISSN: 27686701     EISSN: 27686698     Source Type: Journal    
DOI: 10.2741/1905     Document Type: Article

References (27)

1. Cai W., Hu L., & Foulkes J.G.: Transcription-modulating drugs: mechanism and selectivity. Curr. Opin. Biotechnol. 7, 608-615 (1996)
2. Darnell J.E., Jr.: Transcription factors as targets for cancer therapy. Nat. Rev. Cancer 2, 740-749 (2002)
3. Kovacevic G.N., Mojsin M., Krstic A., & Stevanovic M.: Functional characterization of the human SOX3 promoter: identification of transcription factors implicated in basal promoter activity. Gene 344, 287-297 (1-3-2005)
4. Zhu X., Jiang J., Shen H., Wang H., Zong H., Li Z., Yang Y., Niu Z., Liu W., Chen X., Hu Y., & Gu J.: Elevated beta1,4-galactosyltransferase I in highly metastatic human lung cancer cells. Identification of E1AF as important transcription activator. J. Biol. Chem. 280, 12503-12516 (4-1-2005)
5. En-Nia A., Yilmaz E., Klinge U., Lovett D.H., Stefanidis I., & Mertens P.R.: Transcription factor YB-1 mediates DNA polymerase alpha gene expression. J. Biol. Chem. 280, 7702-7711 (3-4-2005)
6. Li Z., Niu J., Uwagawa T., Peng B., & Chiao P.J.: Function of polo-like kinase 3 in NF-kappaB-mediated proapoptotic response. J. Biol. Chem. 280, 16843-16850 (4-29-2005)
7. Sun H.B., Malacinski G.M., & Yokota H.: Promoter competition assay for analyzing gene regulation in joint tissue engineering. Front Biosci. 7, a169-a174 (8-1-2002)
8. Hube F., Reverdiau P., Iochmann S., Cherpi-Antar C., & Gruel Y.: Characterization and functional analysis of TFPI-2 gene promoter in a human choriocarcinoma cell line. Thromb. Res. 109, 207-215 (2-15-2003)
9. Quandt K., Frech K., Karas H., Wingender E., & Werner T.: MatInd and MatInspector: new fast and versatile tools for detection of consensus matches in nucleotide sequence data. Nucleic Acids Res. 23, 4878-4884 (12-11-1995)
10. Wingender E., Chen X., Fricke E., Geffers R., Hehl R., Liebich I., Krull M., Matys V., Michael H., Ohnhauser R., Pruss M., Schacherer F., Thiele S., & Urbach S.: The TRANSFAC system on gene expression regulation. Nucleic Acids Res. 29, 281-283 (1-1-2001)
11. Fimmel S., Saborowski A., Orfanos C.E., & Zouboulis C.C.: Development of efficient transient transfection systems for introducing antisense oligonucleotides into human epithelial skin cells. Horm. Res. 54, 306-311 (2000)
12. Giles R.V., Spiller D.G., Grzybowski J., Clark R.E., Nicklin P., & Tidd D.M.: Selecting optimal oligonucleotide composition for maximal antisense effect following streptolysin O-mediated delivery into human leukaemia cells. Nucleic Acids Res. 26, 1567-1575 (4-1-1998)
13. Ferber M.J. & Maher L.J., III: Quantitating oligonucleotide affinities for duplex DNA: footprinting vs electrophoretic mobility shift assays. Anal. Biochem. 244, 312-320 (1-15-1997)
14. Peng B., Lu B., Leygue E., & Murphy L.C.: Putative functional characteristics of human estrogen receptor-beta isoforms. J. Mol. Endocrinol. 30, 13-29 (2003)
15. Moxley R.A., Jarrett H.W., & Mitra S.: Methods for transcription factor separation. J. Chromatogr. B Analyt. Technol. Biomed Life Sci. 797, 269-288 (11-25-2003)
16. Moxley R.A. & Jarrett H.W.: Oligonucleotide trapping method for transcription factor purification systematic optimization using electrophoretic mobility shift assay. J. Chromatogr. A 1070, 23-34 (4-8-2005)
17. Rocha A., Ruiz S., & Coll J.M.: Improvement of DNA transfection with cationic liposomes. J. Physiol Biochem. 58, 45-56 (2002)
18. Zhao D.D., Watarai S., Lee J.T., Kouchi S., Ohmori H., & Yasuda T.: Gene transfection by cationic liposomes: comparison of the transfection efficiency of liposomes prepared from various positively charged lipids. Acta Med. Okayama 51, 149-154 (1997)
19. Leroy C., Manen D., Rizzoli R., Lombes M., & Silve C.: Functional importance of Myc-associated zinc finger protein for the human parathyroid hormone (PTH)/PTH-related peptide receptor-1 P2 promoter constitutive activity. J. Mol. Endocrinol 32, 99-113 (2004)
20. Hesselbrock D.R., Kurpios N., Hassell J.A., Watson M.A., & Fleming T.P.: PEA3, AP-1, and a unique repetitive sequence all are involved in transcriptional regulation of the breast cancer-associated gene, mammaglobin. Breast Cancer Res. Treat. 89, 289-296 (2005)
21. Cheng S. & Balk S.P.: Steroid hormone receptor signaling in cancer. Cancer Treat. Res. 115, 293-318 (2003)
22. Favorov A.V., Gelfand M.S., Gerasimova A.V., Ravcheev D.A., Mironov A.A., & Makeev V.J.: A Gibbs sampler for identification of symmetrically structured, spaced DNA motifs with improved estimation of the signal length. Bioinformatics. 21, 2240-2245 (5-15-2005)
23. Pilpel Y., Sudarsanam P., & Church G.M.: Identifying regulatory networks by combinatorial analysis of promoter elements. Nat. Genet. 29, 153-159 (2001)
24. Boulon S., Dantonel J.C., Binet V., Vie A., Blanchard J.M., Hipskind R.A., & Philips A.: Oct-1 potentiates CREB-driven cyclin D1 promoter activation via a phospho. Mol. Cell Biol. 22, 7769-7779 (2002)
25. Cicatiello L., Addeo R., Sasso A., Altucci L., Petrizzi V.B., Borgo R., Cancemi M., Caporali S., Caristi S., Scafoglio C., Teti D., Bresciani F., Perillo B., & Weisz A.: Estrogens and progesterone promote persistent CCND1 gene activation during G1 by inducing transcriptional derepression via c-Jun/c-Fos/estrogen receptor (progesterone receptor) complex assembly to a distal regulatory element and recruitment of cyclin D1 to its own gene promoter. Mol. Cell Biol. 24, 7260-7274 (2004)
26. Voss J.W., Wilson L., & Rosenfeld M.G.: POU-domain proteins Pit-1 and Oct-1 interact to form a heteromeric complex and can cooperate to induce expression of the prolactin promoter. Genes Dev. 5, 1309-1320 (1991)
27. Zhao F.Q., Adachi K., & Oka T.: Involvement of Oct-1 in transcriptional regulation of beta-casein gene expression in mouse mammary gland. Biochim. Biophys. Acta 1577, 27-37 (8-19-2002)