A reverse transcriptase-polymerase chain reaction method improving the specific amplification of caveolin-1 gene sequences

Chinese Journal of Medical Genetics

Volumn 23, Issue 1, 2006, Pages 59-62

  Xu, Yang Yan ^a,b   Yang, Hui Ling ^b,c   You, Yong ^b   Qin, Li ^a   Tu, Jian ^a  

^a UNIVERSITY OF SOUTH CHINA   (China)

^b THE FIRST AFFILIATED HOSPITAL OF UNIVERSITY OF SOUTH CHINA   (China)

^c CENTRAL SOUTH UNIVERSITY   (China)

Author keywords

Phosphorothioate; Polymerase chain reaction; Proofreading polymerase

Indexed keywords

CAVEOLIN 1; DNA POLYMERASE; PHOSPHOROTHIOIC ACID; PRIMER DNA;

ARTICLE; CONTROLLED STUDY; GENE AMPLIFICATION; GENE SEQUENCE; HUMAN; HUMAN CELL; MISMATCH REPAIR; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY;

ANIMALS; CAVEOLIN 1; DEOXYRIBONUCLEASES; GENE AMPLIFICATION; HUMANS; MICE; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; THIONUCLEOTIDES;

EID: 32644434574     PISSN: 10039406     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (7)

1. Yang HL, Jiang HJ, Fang WY, et al. High fidelity PCR with an off/on switch mediated by proofreading polymerases combining with phosphorothioate modified primer. Biochem Biophys Res Commun, 2005, 328:265-272.
2. Skerra A. Phosphorothioate primers improve the amplification of DNA sequences by DNA polymerases with proofreading activity. Nucleic Acids Res, 1992, 20:3551-3554.
3. Zhang L, Zhou W, Velculescu VE, et al. Gene expression profiles in normal and cancer cells. Science, 1997, 276:1268-1272.
4. Kwok S, Kellog DE, Mckinney N, et al. Effects of primer-template mismatches on the polymerase chain reaction: HIV-1 model studies. Nucleic Acids Res, 1990, 17:2503-2516.
5. Goodman MF, Creighton S, Bloom LB, et al. Biochemical basis of DNA replication fidelity. Crit Rev Biochem Mol Biol, 1993, 28:83-126.
6. Boosalis MS, Petruska J, Goodman MF. DNA polymerase insertion fidelity. Gel assay for site-specific kinetics. J Biol Chem, 1987, 262:14689-14696.
7. Mendelman LV, Boosalis MS, Petruska J, et al. Nearest neighbor influences on DNA polymerase insertion fidelity. J Biol Chem, 1989, 264:14415-14423.