Optimization of immunohistochemistry for Bovine Herpesvirus 5 (BHV-5) detection on formalin-fixed tissues of the central nervous system;Otimização da imunoistoquímica para detecção de herpesvírus bovino tipo 5 (BHV-5) em tecidos do sistema nervoso central fixados com formaldeído

Arquivo Brasileiro de Medicina Veterinaria e Zootecnia

Volumn 57, Issue 1, 2005, Pages 1-6

  Hubner S O ^a   Pescador, C ^b   Corbellini, L G ^b   Driemeier, D ^b   Spilki, F R ^b   Roehe, P M ^b,c  

^a FEDERAL UNIVERSITY OF PELOTAS   (Brazil)

^b FEDERAL UNIVERSITY OF RIO GRANDE DO SUL   (Brazil)

^c FEPAGRO   (Brazil)

Author keywords

Antigen retrieval; BHV 5; Central nervous system; Immunohistochemistry

Indexed keywords

BOVINE HERPESVIRUS 5; EQUIDAE; HERPESVIRIDAE; STREPTOMYCES GRISEUS; TRITIRACHIUM ALBUM;

EID: 32444441782     PISSN: 01020935     EISSN: 01020935     Source Type: Journal    
DOI: 10.1590/s0102-09352005000100001     Document Type: Article

References (25)

1. BATTIFORA, H.; KOPINSKI, M. The influence of protease digestion and duration of fixation on the immunostaining of keratins. A comparison of formalin and ethanol fixation. J. Histochem. Cytochem., v.34, p.1095-1100, 1986.
2. BULLOCK, G.R.; PETRUSZ, P. Techniques in immunocytochemistry. 4.ed. San Diego: Academic, 1989. 306p.
3. COLODEL, E.M.; NAKASATO, L.; WEIBLEN, R. et al. Ciên. Rural, v.32, p.293-298, 2002.
4. D'ARCE, R.C.F.; ALMEIDA, R.S.; SILVA, T.C. et al. Restriction endonuclease and monoclonal antibody analysis of Brazilian isolates of bovine herpesviruses types 1 and 5. Vet. Microbiol., v.88, p.315-324, 2002.
5. DAPSON, R.W. Fixation for the 1990's: a review of needs and accomplishments. Biotech. Histochem., v.18,p.11-16, 1993.
6. DARLING, D.C.; MORGAN, S.J. Animal cells - culture and media. London: Wiley, 1994. 151p.
7. D'OFFAY, J.M.; ELY, R.W.; BALDWIN, C.A. et al. Diagnosis of encephalitic bovine herpesvirus type 5 (BHV-5) infection in cattle: virus isolation and immunohistochemical detection of antigen in formalin-fixed bovine brain tissues. J. Vet. Diagn. Invest., v.7, p.247-251, 1995.
8. EBELING, W.; HENRICH, N.; KLOCKW, M. et al. Proteinase K from Tritirachium album Limber. Europ. J. Biochem., v.47,p.91-97, 1974.
9. ELY, R.W.; D'OFFAY, J.M.; RUEFER, A.H. et al. Bovine herpesviral encephalitis: a retrospective study on archived formalin-fixed, paraffin-embedded brain tissue. J. Vet. Diagn. Invest., v.8, p.487-492, 1996.
10. FOX, C.H.; JOHNSON, F.B.; WHITING, J. et al. Formaldehyde fixation. J. Histochem. Cytochem., v.33, p.845-853, 1985.
11. FRENCH, E.L.A. A specific virus encephalithis in calves: isolation and characterization of the causal agent. Aust. Vet. J., v.38, p.216-221, 1962.
12. GUTIERREZ, M.; FORSTER, F.I.; MCCONNEL, S.A. et al. The detection of CD2+, CD4+, CD8+ and WC1+T lymphocytes, B cells and macrophages in fixed and paraffin embedded bovine tissue using a range of antigen recovery and signal amplification techniques. Vet. Immunol. Immunopathol., v.71, p.321-334, 1999.
13. KOK., L.P.; BOON, M.E. Microwave cookbook for microscopists. 3.ed. Leyden: Coloumb, 1992.432p.
14. MACINTYRE, N. Unmasking antigens for immunohistochemistry. Br. J. Biomed. Sci., v.8, p.190-196, 2001.
15. MASON, J.T.; O'LEARY, T.J. Effects of formaldehyde fixation on protein secundary structure: a colorimetric and infrared spectroscopic investigation. J. Histochem. Cytochem., v.39, p.225-229, 1991.
16. MÉNDEZ, M. C.; RIET-CORREA, F.; SCHILD, A. L. et al. Doenças diagnosticadas no ano de 1986. Boletim do Laboratório Regional de Diagnóstico. Pelotas: Ed. da UFPel, 1987. P.30.
17. MEYER, G.; LEMAIRE, M.; ROS, C. et al. Comparative pathogenesis of acute and latent infectious of calves with bovine herpesvirus types 1 and 5. Arch. Virol., v.146, p.633-652, 2001.
18. OLDONI, I.; FLORES, E.F.; WEIBLEN, R. et al. Production and characterization of monoclonal antibodies to a brazilian bovine herpesvirus type 5 (BHV-5) isolate. In: ENCONTRO NACIONAL DE VIROLOGIA, 2002, São Paulo. Anais... São Paulo, p.117. Resumo.
19. RAMOS-VARA, J.A.; BEISSENHERZ, M.E. Antigen retrieval methods to optimize diagnostic immunohistochemistry: experience with 63 markers. J. Vet. Diagn. Invest., v.12, p.307-311, 2000.
20. RIET-CORREA, F.; RIET-CORREA, G.; SCHILD, A.L. Importância do exame clinico para o diagnóstico das enfermidades do sistema nervoso em ruminantes e eqüídeos. Pesq. Vet. Bras., v.22, p.161-168, 2002.
21. RIET-CORREA, F.; VIDOR, T.; SCHILD, A.L. et al. Meningoencefalite e necrose do cortex cerebral em bovinos causadas por herpes virus bovino -1. Pesq. Vet. Bras., v.9, p.13-16, 1989.
22. ROELS, S.; CHARLIER, G.; LETELLIER, C. et al. Natural case of bovine herpesvirus 1 meningoencephalitis in an adult cow. Vet. Rec., v.13, p.586-588, 2000.
23. SALVADOR, S.C.; LEMOS, R.A.; RIET-CORREA, F. et al. Meningoencefalite em bovinos causada por herpesvírus bovino-5 no Mato Grosso do Sul e São Paulo. Pesq. Vet. Bras., v.18, p.76-83, 1998.
24. SHI, S-R.; COTE, R.J.; TAYLOR, C.R. Antigen retrieval immunohistochemistry: past, present and future. J. Histochem. Cytochem., v.45, p.327-343, 1997.
25. SHI, S-R.; KEY, M.E.; KALRA, K.L Antigen retrieval in formalin-fixed, paraffin-embedded tissues: an enhacement method for immunohistochemical staining based on microwave oven heating of tissue sections. J. Histochem. Cytochem., v.39, p.741-748, 1991.