Expression, purification and catalytic activity of Lupinus luteus asparagine β-amidohydrolase and its Escherichia coli homolog

European Journal of Biochemistry

Volumn 271, Issue 15, 2004, Pages 3215-3226

  Borek, Dominika ^a   Michalska, Karolina ^a   Brzezinski, Krzysztof ^a   Kisiel, Agnieszka ^b   Podkowinski, Jan ^b   Bonthron, David T ^c   Krowarsch, Daniel ^d   Otlewski, Jacek ^d   Jaskolski, Mariusz ^a,b  

^a ADAM MICKIEWICZ UNIVERSITY   (Poland)

^b INSTITUTE OF BIOORGANIC CHEMISTRY   (Poland)

^c UNIVERSITY OF LEEDS   (United Kingdom)

^d UNIVERSITY OF WROC AW   (Poland)

Author keywords

Asparaginase; Aspartylglucosaminidase; Glutathione; Isoaspartyl peptidase; Ntn hydrolase

Indexed keywords

ASPARAGINASE; DIPEPTIDASE; GLUCOSAMINIDASE; ISOASPARTYL DIPEPTIDASE; N4 (BETA N ACETYLGLUCOSAMINYL)ASPARAGINASE; THREONINE; UNCLASSIFIED DRUG;

AMINO ACID SEQUENCE; AMINO TERMINAL SEQUENCE; ARTICLE; CATALYSIS; CONTROLLED STUDY; ENZYME ACTIVE SITE; ENZYME ASSAY; ENZYME KINETICS; ENZYME PURIFICATION; ESCHERICHIA COLI; LUPIN; MASS SPECTROMETRY; NONHUMAN; PHYLOGENY; PRIORITY JOURNAL; PROTEIN EXPRESSION; SEQUENCE ANALYSIS; SEQUENCE HOMOLOGY; THERMOSTABILITY;

AMINO ACID SEQUENCE; ASPARTYLGLUCOSYLAMINASE; CATALYSIS; ENZYME STABILITY; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; KINETICS; LUPINUS; MOLECULAR SEQUENCE DATA; MOLECULAR STRUCTURE; PHYLOGENY; PROTEIN DENATURATION; SEQUENCE ALIGNMENT; SPECTROMETRY, MASS, ELECTROSPRAY IONIZATION; SUBSTRATE SPECIFICITY; TEMPERATURE;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; LUPINUS; LUPINUS LUTEUS; NEGIBACTERIA;

EID: 3242893114     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1432-1033.2004.04254.x     Document Type: Article

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