Simultaneous in situ detection of mRNA and apoptotic cells by combined hybridization and TUNEL

Journal of Histochemistry and Cytochemistry

Volumn 44, Issue 12, 1996, Pages 1497-1499

  Sträter, Jörn ^a   Walczak, Henning ^b   Krammer, Peter H ^b   Möller, Peter ^a  

^a UNIVERSITY OF ULM   (Germany)

^b GERMAN CANCER RESEARCH CENTER DKFZ   (Germany)

Author keywords

Apoptosis; Gene expression; Human; In situ hybridization; mRNA; Paraffin sections; Programmed cell death; TUNEL

Indexed keywords

BIOTIN; DEOXYURIDINE TRIPHOSPHATE DERIVATIVE; DIGOXIGENIN; DNA FRAGMENT; DNA NUCLEOTIDYLEXOTRANSFERASE; FAS ANTIGEN; MESSENGER RNA;

APOPTOSIS; ARTICLE; CELL DEATH; CHILD; CONTROLLED STUDY; ETIOLOGY; GENE EXPRESSION; HUMAN; HUMAN TISSUE; IN SITU HYBRIDIZATION; INFANT; MICROSCOPY; NEWBORN; PRIORITY JOURNAL; THYMECTOMY;

EID: 3242872211     PISSN: 00221554     EISSN: None     Source Type: Journal    
DOI: 10.1177/44.12.8985142     Document Type: Article

References (10)

1. Cohen JJ, Duke RC, Fadok VA, Sellins KS (1992) Apoptosis and programmed cell death in immunity. Annu Rev Immunol 10:267-293
2. Dhein J, Daniel PT, Trauth BC, Oehm A, Möller P, Krammer PH (1992) Induction of apoptosis by monoclonal antibody anti-APO-1 class switch variants is dependent on cross-linking of APO-1 cell surface antigens. J Immunol 149:3166-3173
3. Gavrieli Y, Sherman Y, Ben-Sasson SA (1992) Identification of programmed cell death in situ via specific labeling of nuclear DNA fragmentation. J Cell Biol 119:493-501
4. Kerr JFR (1971) Shrinkage necrosis: a distinct mode of cellular death. J Pathol 105:13-20
5. Kerr JFR, Wyllie AH, Currie AR (1972) Apoptosis, a basic biological phenomenon with wider implications in tissue kinetics. Br J Cancer 26:239-257
6. Oltvai ZN, Korsmeyer SJ (1994) Checkpoints of dueling dimers foil death wishes. Cell 79:189-192
7. Sträter J, Günthert AR, Brüderlein S, Möller P (1995) Microwave irradiation of paraffin-embedded tissue sensitizes the TUNEL method for the in situ detection of apoptotic cells. Histochem Cell Biol 103:157-160
8. Suda T, Takahaski T, Golstein P, Nagata S (1993) Molecular cloning and expression of the Fas ligand, a novel member of the tumor necrosis factor family. Cell 75:1169-1178
9. Wijsman JH, Jonker RR, Keijzer R, van de Velde CJH, Cornelisse CJ, van Dierendonck JH (1993) A new method to detect apoptosis in paraffin sections: in situ end-labeling of fragmented DNA. J Histochem Cytochem 41:7-12
10. Wyllie AH (1980) Glucocorticoid-induced thymocyte apoptosis is associated with endogenous endonuclease activation. Nature 284:555-556