Histochemical and immunohistochemical detection of neurons that produce nitric oxide: Effect of different fixative parameters and immunoreactivity against non-neuronal NOS antisera

Journal of Histochemistry and Cytochemistry

Volumn 44, Issue 12, 1996, Pages 1399-1413

  Gonzalez Hernandez, Tomas ^a   De La Cruz, Maria Angeles Perez ^b   Mantolan Sarmiento, Beatriz ^a  

^a UNIVERSIDAD DE LA LAGUNA   (Spain)

^b UNIVERSITY OF SALAMANCA   (Spain)

Author keywords

Double labeling; Fixation; macNOS nNOS co localization; NADPH diaphorase; Nitric oxide; NOS isoforms

Indexed keywords

FIXATIVE; NITRIC OXIDE; NITRIC OXIDE SYNTHASE; PARAFORMALDEHYDE; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE DEHYDROGENASE;

ANIMAL TISSUE; ANTIBODY LABELING; ARTICLE; BRAIN NERVE CELL; BRAIN PERFUSION; CONTROLLED STUDY; ENDOTHELIUM CELL; ENZYME ACTIVITY; HISTOCHEMISTRY; IMMUNOHISTOCHEMISTRY; MACROPHAGE; NONHUMAN; PRIORITY JOURNAL; RAT; TISSUE FIXATION;

EID: 3242855995     PISSN: 00221554     EISSN: None     Source Type: Journal    
DOI: 10.1177/44.12.8985132     Document Type: Article

References (54)

1. Bredt DS, Glatt CE, Hwang PM, Fotuhi M, Dawson TM, Snyder SH (1991) Nitric oxide synthase protein and mRNA are discretely localized in neuronal populations of the mammalian CNS together with NADPH diaphorase. Neuron 7:615-624
2. Bredt DS, Hwang PM, Snyder SH (1990) Localization of nitric oxide synthase indicating a neural role for nitric oxide. Nature 347:768-770
3. Bredt DS, Snyder SH (1992) Nitric oxide, a novel neuronal messenger. Neuron 8:3-11
4. Bredt DS, Snyder SH (1990) Isolation of nitric oxide synthetase, a calmodulin-requiring enzyme. Proc Natl Acad Sci USA 87:682-685
5. Buwalda B, Nyakas C, Gast J, Luiten PGM, Schmidt HHHW (1995) Aldehyde fixation differentially affects distribution of diaphorase activity but not of nitric oxide synthase immunoreactivity in rat brain. Brain Res Bull 38:467-473
6. Dawson TM, Bredt DS, Fotuhi M, Hwang PM, Snyder SH (1991) Nitric oxide synthase and neuronal NADPH diaphorase are identical in brain and peripherical tissues. Proc Natl Acad Sci USA 88:7797-7801
7. Dawson TM, Dawson VL, Snyder SH (1992) A novel neuronal messenger molecule in brain: the free radical, nitric oxide. Ann Neurol 32:297-311
8. Decker K, Reuss S (1994) Nitric oxide-synthesizing neurons in the hamster suprachiasmatic nucleus: a combined NOS- and NADPH-staining and retinohypothalamic tract tracing study. Brain Res 666:284-288
9. Dinermann YL, Dawson TM, Schell MJ, Snowman A, Snyder SH (1994) Endothelial nitric oxide synthase localized to hippocampal pyramidal cells: implications for synaptic plasticity. Proc Natl Acad Sci USA 91:4214-4218
10. Di Prospero NA, Geller HM (1995) The presence of inducible nitric oxide synthase after spinal cord injury. Soc Neurosci Abst 22:232
11. Dun NJ, Dun SL, Förstermann U, Tseng LF (1992) Nitric oxide synthase immunoreactivity in rat spinal cord. Neurosci Lett 147:217-220
12. Endoh M, Maiese K, Wagner JA (1994a) Expression of the neuronal form of nitric oxide synthase by CA1 hippocampal neurons and other central nervous system neurons. Neuroscience 63:679-689
13. Endoh M, Maiese K, Wagner J (1994b) Expression of the inducible form of nitric oxide synthase by reactive astrocytes after transient global ischemia. Brain Res 651:92-100
14. Förstermann U, Pollock JS, Schmidt HHHW, Heller M, Murad F (1991a) Calmodulin-dependent endothelium derived relaxing factor/nitric oxide synthase activity is present in the particulate and cytosolic fractions of bovine aortic endothelial cells. Proc Natl Acad Sci USA 88:1788-1792
15. Förstermann U, Schmidt HHHW, Pollock J, Sheng H, Mitchell JA, Warner TD, Nakane M, Murad F (1991b) Isoforms of nitric oxide synthase: characterization and purification from different cell types. Biochem Pharmacol 42:1849-1857
16. Galea E, Reis DJ, Xu H, Feinstein DL (1995) transient expression of calcium-independent nitric oxide synthase in blood vessels during brain development. FASEB J 9:1632-1637
17. Garthwaite J (1991) Glutamate, nitric oxide and cell-cell signalling in the nervous system. Trends Neurosci 14:60-67
18. Garthwaite J, Boulton CL (1995) Nitric oxide signaling in the central nervous system. Annu Rev Physiol 57:683-706
19. Hashikawa T, Leggio MG, Hattori R, Yui Y (1994) Nitric oxide synthase immunoreactivity colocalized with NADPH-diaphorase histochemistry in monkey cerebral cortex. Brain Res 641:341-349
20. Hope BT, Michael GJ, Knigge KM, Vincent SR (1991) Neuronal NADPH diaphorase is a nitric oxide synthase. Proc Natl Acad Sci USA 88:2811-2814
21. 450 reductase. Biochem Pharmacol 37:3063-3070
22. Kharazia VN, Schmidt HHHW, Weinberg RJ (1994) Type I nitric oxide synthase fully accounts for NADPH-diaphorase in rat striatum, but not cortex. Neuroscience 62:983-987
23. Koprowski H, Zeng YM, Heber-Katz E, Fraser N, Rorke L, Fu KF, Hanlon C, Dietzschold B (1993) In vivo expression of inducible nitric oxide synthase in experimentally induced neurologic diseases. Proc Natl Acad Sci USA 90:3024-3027
24. Lamas S, Marsdan PA, Li GK, Tempst P, Michel T (1992) Endothelial nitric oxide synthase: molecular cloning and characterization of a distinct constitutive enzyme isoform. Proc Natl Acad Sci USA 89:6348-6352
25. Levey AL, Bolam JP, Rye DB, Hallanger AE, Demuth RM, Mesulam MM, Wainer BH (1986) A light and electron microscopic procedure for sequential double antigen localization using diaminobenzidine and benzidine dihydrochloride. J Histochem Cytochem 34:1449-1457
26. Lowenstein CJ, Glatt CS, Bredt DS, Snyder SH (1992) Cloned and expressed macrophage nitric oxide synthase contrasts with the brain enzyme. Proc Natl Acad Sci USA 89:6711-6715
27. Matsumoto T, Nakane M, Pollock JS, KukJE, Förstermann U (1993) A correlation between soluble brain nitric oxide synthase and NADPH-diaphorase activity is only seen after exposure of the tissue to fixative. Neurosci Lett 155:61-64
28. McKee M, Scavone C, Nathanson JA (1994) Nitric oxide, cGMP, and hormone regulation of active sodium transport. Proc Natl Acad Sci USA 91:12056-12060
29. Moncada S, Higgs A (1993) The 1-arginine-nitric oxide pathway. N Engl J Med 329:2002-2012
30. Moncada S, Palmer RMJ, Higgs E (1991) Nitric oxide: physiology, pathophysiology, and pharmacology. Pharmacol Rev 43:109-142
31. Nakane M, Schmidt HHHW, Warner DT, Huang ZJ, Murad F (1991) Induction of type II guanylyl cyclase-activating factor (GAP) synthase in raw macrophages requires transcription and de novo protein biosynthesis. FASEB J 5;A509
32. Nathan C, Xie Q-W (1994) Regulation of biosynthesis of nitric oxide. J Biol Chem 269:13725-13728
33. Paxinos G, Watson C (1986) The Rat Brain in Stereotaxic Coordinates. Sydney, Academic Press
34. Pollock JS, Förstermann U, Tracey WR, Nakane M (1995) Nitric oxide synthase isozyme antibodies. Histochem J 27:738-744
35. Rodrigo J, Springall DR, Uttenthal O, Bentura ML, Abadia-Molina F, Riveros-Moreno V, Martínez-Murillo R, Polak JM, Moncada S (1994) Localization of nitric oxide synthase in the adult rat brain. Phil Trans R Soc Lond [B] 345:175-221
36. Roufail E, Stringer M, Rees S (1995) Nitric oxide synthase immunoreactivity and NADPH diaphorase staining are co-localized in neurons closely associated with the vasculature in rat and human retina. Brain Res 684:36-46
37. Sandell JH (1986) NADPH diaphorase histochemistry in the macaque striate cortex. J Comp Neurol 251:388-397
38. Schmidt HHHW, Gagne GD, Nakane M, Pollock JS, Miller MF, Murad F (1992) Mapping of neural nitric oxide synthase in the rat suggests frequent co-localization with NADPH diaphorase but not with soluble guanylyl cyclase, and novel paraneural functions of nitrinergic signal transduction. J Histochem Cytochem 40:1439-1456
39. Schmidt HHHW, Pollock JS, Nakane M, Gorsky DL, Förstermann U, Murad F (1991) Purification of a soluble isoform of guanylyl cyclase-activating-factor synthase. Proc Natl Acad Sci USA 88:365-369
40. Sims KL, Kauffman FC, Johnson EC, Pickel VM (1974) Cytochemical localization of brain nicotinamide adenine dinucleotide phosphate (oxidized)-dependent dehydrogenases: qualitative and quantitative distributions. J Histochem Cytochem 22:7-19
41. Spessert R, Layes E (1994) Fixation conditions affect the intensity but not the pattern of NADPH-diaphorase staining as a marker for neuronal nitric oxide synthase in rat olfactory bulb. J Histochem Cytochem 42:1309-1315
42. Spessert R, Wohlgemuth C, Reuss S, Layes E (1994) NADPH-diaphorase activity of nitric oxide synthase in the olfactory bulb: co-factor specificity and characterization regarding the interrelation to NO formation. J Histochem Cytochem 42:569-575
43. Tojo A, Gross SS, Zhang L, Tisher CC, Schmidt HHHW, Wilcox CS, Madsen KM (1994) Immunocytochemical localization of distinct isoforms of nitric oxide synthase in the juxtaglomerular apparatus of normal rat kidney. J Am Soc Nephrol 4:1438-1447
44. Traub RJ, Solodkin A, Meller ST, Gebhart GF (1994) Spinal cord NADPH-diaphorase histochemical staining but not nitric oxide synthase immunoreactivity increases following carrageenan-produced hindpaw inflammation in the rat. Brain Res 668:204-210
45. Valtschanoff JG, Weinberg RJ, Kharazia VN, Nakane M, Schmidt HHHW (1993) Neurons in rat hippocampus that synthesize nitric oxide. J Comp Neurol 331:111-121
46. Vincent SR (1995) Localization of nitric oxide neurons in the central nervous system. In Vincent SR, ed. Nitric Oxide in the Nervous System. London, Academic Press, 83-102
47. Vincent SR (1994) Nitric oxide: a radical neurotransmitter in the central nervous system. Prog Neurobiol 42:129-160
48. Vincent SR, Hope BT (1992) Neurons that say NO. Trends Neurosci 15:108-113
49. Vizzard MA, Erdman SL, Roppolo JR, Förstermann U, de Groat WC (1994) Differential localization of neuronal nitric oxide synthase immunoreactivity and NADPH-diaphorase activity in the cat spinal cord. Cell Tissue Res 278:299-309
50. Wallace NM, Fredens K (1992) Activated astrocytes of the mouse hippocampus contain high levels of NADPH-diaphorase. NeuroReport 3:953-956
51. Weinberg RJ, Valtschanoff JG, Schmidt HHHW (1996) The NADPH diaphorase histochemical stain. In Feelisch H, Stamler SS, eds. Methods in Nitric Oxide Research. New York, John Wiley & Sons, 237-248
52. Wendland B, Schweizer FE, Ryan TA, Nakane M, Murad F, Scheller RH, Tsien RW (1994) Existence of nitric oxide synthase in rat hippocampal pyramidal cells. Proc Natl Acad Sci USA 91:2151-2155
53. Xie Q-W, Cho HJ, Calaycay J, Mumford RA, Swiderek KM, Lee TD, Ding A, Troso T, Nathan C (1992) Cloning and characterization of inducible nitric oxide synthase from mouse macrophages. Science 256:225-228
54. Zilles K (1985) The Cortex of the Rat. A Stereotaxic Atlas. Berlin, Springer-Verlag