Degradation of GFP-labelled POM121, a non-invasive sensor of nuclear apoptosis, precedes clustering of nuclear pores and externalisation of phosphatidylserine

Apoptosis

Volumn 9, Issue 3, 2004, Pages 363-368

  Beckman, M ^a   Kihlmark, M ^b   Iverfeldt, K ^a   Hallberg, E ^b  

^a STOCKHOLM UNIVERSITY   (Sweden)

^b SÖDERTÖRN UNIVERSITY   (Sweden)

Author keywords

Annexin V; Apoptosis; Green fluorescent protein; Nuclear envelope; Nuclear pore; Phosphatidylserine

Indexed keywords

CASPASE 3; GREEN FLUORESCENT PROTEIN; LIPOCORTIN 5; MEMBRANE PROTEIN; PHOSPHATIDYLSERINE; PROTEIN POM121; UNCLASSIFIED DRUG;

ANIMAL CELL; APOPTOSIS; ARTICLE; CELL MEMBRANE; CONTROLLED STUDY; FLUORESCENCE; NONHUMAN; NUCLEAR PORE; PHAGOCYTOSIS; PRIORITY JOURNAL; PROTEIN DEGRADATION; PROTEIN EXPRESSION; SENSOR;

ANIMALS; ANNEXIN A5; APOPTOSIS; CASPASES; CELL LINE; CELL NUCLEUS; CRICETINAE; CRICETULUS; ENZYME ACTIVATION; HYDROLYSIS; MEMBRANE GLYCOPROTEINS; MEMBRANE PROTEINS; MICROSCOPY, FLUORESCENCE; MUTATION; NUCLEAR PORE; PC12 CELLS; PHOSPHATIDYLSERINES; RATS; RECOMBINANT PROTEINS; TIME FACTORS;

ANIMALIA;

EID: 3242805164     PISSN: 13608185     EISSN: None     Source Type: Journal    
DOI: 10.1023/B:APPT.0000025813.75258.b5     Document Type: Article

References (18)

1. Ameisen JC. On the origin, evolution, and nature of programmed cell death: A timeline of four billion years. Cell Death Differ 2002; 9: 367-393.
2. Nicholson DW. Caspase structure, proteolytic substrates, and function during apoptotic cell death. Cell Death Differ 1999; 6: 1028-1042.
3. Savill J, Fadok V. Corpse clearance defines the meaning of cell death. Nature 2000; 407: 784-788.
4. Martin SJ, Reutelingsperger CP, McGahon AJ, et al. Early redistribution of plasma membrane phosphatidylserine is a general feature of apoptosis regardless of the initiating stimulus: Inhibition by overexpression of Bcl-2 and Abl. J Exp Med 1995; 182: 1545-1556.
5. Robertson JD, Orrenius S, Zhivotovsky B. Review: Nuclear events in apoptosis. J Struct Biol 2000; 129: 346-358.
6. Falcieri E, Gobbi P, Cataldi A, Zamai L, Faenza I, Vitale M. Nuclear pores in the apoptotic cell. Histochem J 1994; 26: 754-763.
7. Hallberg E, Wozniak RW, Blobel G. An integral membrane protein of the pore membrane domain of the nuclear envelope contains a nucleoporin-like region. J Cell Biol 1993; 122: 513-521.
8. Imreh G, Beckman M, Iverfeldt K, Hallberg E. Noninvasive monitoring of apoptosis versus necrosis in a neuroblastoma cell line expressing a nuclear pore protein tagged with the green fluorescent protein. Exp Cell Res 1998; 238: 371-376.
9. Kihlmark M, Imreh G, Hallberg E. Sequential degradation of proteins from the nuclear envelope during apoptosis. J Cell Sci 2001; 114: 3643-3653.
10. Kihlmark M, Rustum C, Eriksson C, Beckman M, Iverfeldt K, Hallberg E. Correlation between nucleocytoplasmic transport and caspase-3-dependent dismantling of nuclear pores during apoptosis. Exp Cell Res 2004; 293: 346-356.
11. Soderqvist H, Imreh G, Kihlmark M, Linnman C, Ringertz N, Hallberg E. Intracellular distribution of an integral nuclear pore membrane protein fused to green fluorescent protein-localization of a targeting domain. Eur J Biochem 1997; 250: 808-813.
12. Imreh G, Hallberg E. An integral membrane protein from the nuclear pore complex is also present in the annulate lamellae: Implications for annulate lamella formation. Exp Cell Res 2000; 259: 180-190.
13. Davis LI, Blobel G. Identification and characterization of a nuclear pore complex protein. Cell 1986; 45: 699-709.
14. Soderqvist H, Jiang WQ, Ringertz N, Hallberg E. Formation of nuclear bodies in cells overexpressing the nuclear pore protein POM121. Exp Cell Res 1996; 225: 75-84.
15. Martin SJ, Finucane DM, Amarante-Mendes GP, O'Brien GA, Green DR. Phosphatidylserine externalization during CD95-induced apoptosis of cells and cytoplasts requires ICE/CED-3 protease activity. J Biol Chem 1996; 271: 28753-28756.
16. Mandal D, Moitra PK, Saha S, Basu J. Caspase 3 regulates phosphatidylserine externalization and phagocytosis of oxidatively stressed erythrocytes. FEBS Lett 2002; 513: 184-188.
17. Imreh G, Maksel D, de Monvel JB, Branden L, Hallberg E. ER retention may play a role in sorting of the nuclear pore membrane protein POM121. Exp Cell Res 2003; 284: 173-184.
18. Daigle N, Beaudouin J, Hartnell L, et al. Nuclear pore complexes form immobile networks and have a very low turnover in live mammalian cells. J Cell Biol 2001; 154: 71-84.