Use of the riboflavin synthase gene (ribC) as a model for development of an essential gene disruption and complementation system for Haemophilus influenzae

Applied and Environmental Microbiology

Volumn 70, Issue 7, 2004, Pages 4136-4143

  Saeed Kothe, Amna ^a,c   Yang, Wei ^b   Mills, Scott D ^b  

^a NORTHEASTERN UNIVERSITY   (United States)

^b ASTRAZENECA   (United Kingdom)

^c BIOGEN   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CHROMOSOMES; GENES; MATHEMATICAL MODELS; RELIABILITY; VECTORS;

BIOSYNTHETIC PATHWAY; GENETIC ELEMENTS; PHENOTYPE;

MICROBIOLOGY;

CHLORAMPHENICOL; DNA FRAGMENT; GENE PRODUCT; RIBOFLAVIN SYNTHASE; TETRACYCLINE;

MICROBIOLOGY;

ANTIBIOTIC RESISTANCE; ARTICLE; BACTERIAL GENE; BACTERIUM TRANSFORMATION; BINDING SITE; DNA FLANKING REGION; DOWNSTREAM PROCESSING; ESSENTIAL GENE; GENE; GENE CONSTRUCT; GENE DELETION; GENE DISRUPTION; GENE LOCUS; GENETIC COMPLEMENTATION; GENETIC MARKER; GENETIC MODEL; HAEMOPHILUS INFLUENZAE; HAEMOPHILUS INFLUENZAE RD STRAIN KW20; MOLECULAR CLONING; NONHUMAN; OMPP1 GENE; PHENOTYPE; PROMOTER REGION; RELIABILITY; RIBC GENE; STRAIN IDENTIFICATION; SUICIDE GENE;

BACTERIAL PROTEINS; BASE SEQUENCE; CHROMOSOMES, BACTERIAL; GENES, ESSENTIAL; GENETIC COMPLEMENTATION TEST; HAEMOPHILUS INFLUENZAE; MOLECULAR SEQUENCE DATA; NUCLEOTIDYLTRANSFERASES; PHOSPHOTRANSFERASES (ALCOHOL GROUP ACCEPTOR);

BACTERIA (MICROORGANISMS); HAEMOPHILUS; HAEMOPHILUS INFLUENZAE; HAEMOPHILUS INFLUENZAE RD KW20; NEGIBACTERIA;

EID: 3242748400     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/AEM.70.7.4136-4143.2004     Document Type: Article

References (21)

1. Akerley, B. J., E. J. Rubin, V. L. Novick, K. Amaya, N. Judson, and J. J. Mekalanos. 2002. A genome-scale analysis for identification of genes required for growth or survival of Haemophilus influenzae. Proc. Natl. Acad. Sci. USA 99:966-971.
2. Bacher, A., S. Eberhardt, and G. Richter. 1996. Biosynthesis of riboflavin, p. 657-664. In F. C. Neidhardt, R. Curtiss III, J. L. Ingraham, E. C. C. Lin, K. B. Low, B. Magasanik, W. S. Reznikoff, M. Riley, M. Schaechter, and H. E. Umbarger (ed.), Escherichia coil and Salmonella: cellular and molecular biology, 2nd ed. ASM Press, Washington, D.C.
3. Bandrin, S. V., M. Y. Beburov, P. M. Rabinovich, and A. I. Stepanov. 1979. Riboflavin auxotrophs of Escherichia coli. Genetika 15:2063-2065.
4. Barcak, G. J., M. S. Chandler, R. J. Redfield, and J. F. Tomb. 1991. Genetic systems in Haemophilus influenzae. Methods Enzymol. 204:321-342.
5. Fleischmann, R. D., M. D. Adams, O. White, R. A. Clayton, E. F. Kirkness, A. R. Kerlavage, et al. 1995. Whole-genome random sequencing and assembly of Haemophilus influenzae Rd. Science 269:496-512.
6. Georgiou, M., R. Munoz, F. Roman, R. Canton, R. Gomez-Lus, J. Campos, and A. G. De La Campa. 1996. Ciprofloxacin-resistant Haemophilus influenzae strains possess mutations in analogous positions of GyrA and ParC. Antimicrob. Agents Chemother. 40:1741-1744.
7. Jacobs, M. R., D. Felmingham, P. C. Appelbaum, and R. N. Gruneberg. 2003. The Alexander Project 1998-2000: susceptibility of pathogens isolated from community-acquired respiratory tract infection to commonly used antimicrobial agents. J. Antimicrob. Chemother. 52:229-246.
8. Menard, R., P. J. Sansonetti, and C. Parsot. 1993. Nonpolar mutagenesis of the ipa genes defines IpaB, IpaC, and IpaD as effectors of Shigella flexneri entry into epithelial cells. J. Bacteriol. 175:5899-5906.
9. Mills, S. D. 2003. The role of genomics in antimicrobial discovery. J. Antimicrob. Chemother. 51:749-752.
10. Moxon, E. R. 1995. Haemophilus influenzae, p. 2039-2045. In G. L. Mandell, J. E. Bennet, and R. Dolin. (ed.), Principles and practice of infectious diseases, vol. 2. Churchill Livingstone, New York, N.Y.
11. Munson, R., Jr., and A. Hunt. 1989. Isolation and characterization of a mutant of Haemophilus influenzae type b deficient in outer membrane protein P1. Infect. Immun. 57:1002-1004.
12. Munson, R., Jr., and S. Grass. 1988. Purification, cloning, and sequence of outer membrane protein P1 of Haemophilus influenzae type b. Infect. Immun. 56:2235-2242.
13. Reich, K. A., L. Chovan, and P. Hessler. 1999. Genome scanning in Haemophilus influenzae for identification of essential genes. J. Bacteriol. 181:4961-4968.
14. Ren, Z., H. Jin, D. J. Morton, and T. L. Stull. 1998. hgpB, a gene encoding a second Haemophilus influenzae hemoglobin- and hemoglobin-haptoglobin- binding protein. Infect. Immun. 66:4733-4741.
15. Smith, H. O., J. F. Tomb, B. A. Dougherty, R. D. Fleischmann, and J. C. Venter. 1995. Frequency and distribution of DNA uptake signal sequences in the Haemophilus influenzae Rd genome. Science 269:538-540.
16. Spencer, H. T., and R. M. Herriott. 1965. Development of competence of Haemophilus influenzae. J. Bacteriol. 90:911-920.
17. Vitreschak, A. G., D. A. Rodionov, A. A. Mironov, and M. S. Gelfand. 2002. Regulation of riboflavin biosynthesis and transport genes in bacteria by transcriptional and translational attenuation. Nucleic Acids Res. 30:3141-3151.
18. Whitby, P. W., D. J. Morton, and T. L. Stull. 1998. Construction of antibiotic resistance cassettes with multiple paired restriction sites for insertional mutagenesis of Haemophilus influenzae. FEMS Microbiol. Lett. 158:57-60.
19. Wilcox, K. W., and H. O. Smith. 1975. Isolation and characterization of mutants of Haemophilus influenzae deficient in an adenosine 5′- triphosphate-dependent deoxyribonuclease activity. J. Bacteriol. 122:443-453.
20. Yanisch-Perron, C., J. Vieira, and J. Messing. 1985. Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene 33:103-119.
21. Zhanel, G. G., J. A. Karlowsky, D. E. Low, and D. J. Hoban. 2000. Antibiotic resistance in respiratory tract isolates of Haemophilus influenzae and Moraxella catarrhalis collected from across Canada in 1997-1998. J. Antimicrob. Chemother. 45:655-662.