HSP-72 and ubiquitin expression and caspase-3 activity after a single boutof eccentric exercise

Journal of Exercise Physiology Online

Volumn 6, Issue 2, 2003, Pages 96-104

  Willoughby, Darryn S ^a   Rosene, John ^b   Myers, Jay ^c  

^a TEXAS CHRISTIAN UNIVERSITY   (United States)

^b UNIVERSITY OF HAWAII   (United States)

^c UNIVERSITY OF SOUTHERN MAINE   (United States)

Author keywords

Gene expression; Muscle injury; Proteolysis

Indexed keywords

CASPASE 3; HEAT SHOCK PROTEIN 72; MESSENGER RNA; MUSCLE PROTEIN; UBIQUITIN;

ADULT; APOPTOSIS; ARTICLE; BLOOD SAMPLING; CONTROLLED STUDY; ENZYME ACTIVITY; EXERCISE; EXTENSOR MUSCLE; HUMAN; HUMAN TISSUE; KNEE; MALE; MUSCLE BIOPSY; MUSCLE FATIGUE; MUSCLE STRENGTH; NORMAL HUMAN; PHYSICAL ACTIVITY; PROTEIN DEGRADATION; PROTEIN EXPRESSION; UPREGULATION;

EID: 3242723150     PISSN: 10979751     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (27)

1. Mair, J., Koller, A., Artner-Dworzak, E., Haid, C., Wicke, K., Judmaier, W., and Puschendorf, B. Effects of exercise on plasma myosin heavy chain fragments and MRI of skeletal muscle. J Appl Physiol 1992;72:656-663.
2. Sorichter, S., Mair, J., Koller, A., Gebert, W., Rama, D., Calzolari, C., Artner-Dworzak, E., and Puschendorf, B. Skeletal troponin I as a marker of exercise-induced muscle damage. J Appl Physiol 1997;83:1076-1082.
3. Biral, D., Jakubiec-Puka, A., Ciechomska, I., Sandri, M., Rossini, K., Carraro, U., and Betto, R. Loss of dystrophin and some dystrophin-associated proteins with concomitant signs of apoptosis in rat leg muscle overworked in extension. Acta Neuropatho. 2000;100:618-626.
4. Bercovich, B., Stancovski, I., Mayer, A., Blumenfeld, N., Laszlo, A., Schwartz, A., and Ciechanover, A. Ubiquitin-dependent degradation of certain protein substrates in vitro requires the molecular chaperone Hsc70. J Biol Chem 1997;272:9002-9010.
5. Thompson, H., and Scordilis, S. Ubiquitin changes in human biceps muscle following exercise-induced damage. Bioch Biophy R. Com 1994;204:1193-1198.
6. Morimoto, R. Cells in stress: transcriptional activation heat shock genes. Science 1993;259:1409-1410.
7. Thompson, H., Scordilis, S., Clarkson, P., and Lohrer, W. A single bout of eccentric exercise increases HSP27 and HSC/HSP70 in human skeletal muscle. Acta Physiol Scand 2001;171:187-193.
8. Willoughby, D., and Nelson, M. Myosin heavy chain isoform and muscle transcription factor expression after a single bout of heavy resistance exercise. Med Sci Sports Exerc (In Press).
9. Willoughby, D., and Rosene, J. Effects of oral creatine and heavy resistance training on myosin heavy chain expression. Med. Sci. Sports Exerc 2001;33:1674-1681.
10. Chomczynski, P., and Sacchi, N. Single-step method of RNA isolation by acid guanidium thiocynate-phenol-chloroform extraction. Ana. Biochem 1987;162:156-59.
11. Willoughby, D., Priest, J., and Nelson, M. Expression of the stress proteins, ubiquitin, HSP-72, and myofibrillar protein content after 12 weeks of leg cycling in persons with spinal cord injury. Arch Phys. Med Rehab 2002;83:649-654.
12. Current Protocols in Molecular Biology. Short Protocols in Molecular Biology. pp. A3: 10-11. New York: Wiley and Sons, 1999.
13. Willoughby, D., Priest, J., and Jennings, R. Myosin heavy chain isoform and ubiquitin protease mRNA expression after passive leg cycling in individuals with spinal cord injury. Arch Phys Med Rehab 2000;81:157-63.
14. Puntschart, A., Claassen, H., Jostarndt, K., Hoppeler, H., Billeter, R. mRNAs of enzymes involved in energy metabolism and mtDNA are increased in endurance-trained athletes. Am J Physiol 1995;269:C619-25.
15. Wright, C., Haddad, F., Qin, A., and Baldwin, K. Analysis of myosin heavy chain mRNA expression by RT-PCR. J Appl Physiol 1997;83:1389-1396.
16. Welle, S., Bhatt, K. and Thornton, C. Stimulation of myofibrillar synthesis by exercie is mediated by more efficient translation of mRNA. J Appl Physiol 1999;86:1220-1225.
17. Bradford, M. A rapid and sensitive method for the quantification of microgram quantitites of protein using the principle of protein dye binding. Ann Biochem 1976;72:248-254.
18. Phaneuf, S., and Leeuwenburgh, C. Apoptosis and exercise. Med Sci Sports Exerc 2001;33:393-396.
19. Febbraio, M., and Koukoulas, I. HSP72 gene expression progressively increases in human skeletal muscle during prolonged, exhaustive exercise. J Appl Physiol 2000;89:1055-1060.
20. Hobler, S., Tiao, G., Fischer, J., Monaco, J., Hasselgren, P. Sepsis-induced increase in muscle proteolysis is blocked by specific proteasome inhibitors. Am J Physiol 1998;274:R30-R37.
21. Sandri, M., Carraro, U., Podhorska-Okolov, M., Rizzi, C., Arslan, P., Monti, D., and Franceschi, C. Apoptosis, DNA damage and ubiquitin expression in normal and mdx muscle fibers after exercise. FEBS Lett 1995;373:291-295.
22. Stupka, N., Tarnopolsky, M., Yardley, N., and Phillips, S. Cellular adaptation to repeated eccentric exercise-induced muscle damage. J Appl Physiol 2001;91:1669-1678.
23. Mosser, D., Caron, A., Bourget, L., Denis-Larose, C., and Massie, B. Role of the human heat shock protein hsp70 in protection against stress-induced apoptosis. Mol Cell Biol 1997;17:5317-5327.
24. Gething, M., and Sambrook, J. Protein folding in the cell. Nature 1992;355:33-45.
25. Puntschart, A., Voght, M., Widmer, H., Hoppeler, H., and Billeter, R. HSP70 expression in human skeletal muscle after exercise. Acta Physiol Scand 1996;157:411-417.
26. Liu, Y., Lormes, W., Baur, C., Opitz-Gress, D., Altenburg, D., Lehmann, M., Steinacker, J. Human skeletal muscle HSP70 response to physical training depends of exercise intensity. Int J Sports Med 2000;21:351-355.
27. Newham, D., McPhail, G, Mills, K., and Edwards, R. Ultrastructural changes after concentric and eccentric contractions of human muscle. J Neurol Sci 1983;61:109-122.