Identification of ciprofloxacin-resistant Campylobacter jejuni and analysis of the gyrA gene by the LightCycler mutation assay

Molecular and Cellular Probes

Volumn 18, Issue 4, 2004, Pages 255-261

  Dionisi, Anna Maria ^a   Luzzi, Ida ^a   Carattoli, Alessandra ^a  

^a ISTITUTO SUPERIORE DI SANITÀ   (Italy)

Author keywords

Campylobacter; Ciprofloxacin resistance; gyrA mutation; Real time PCR

Indexed keywords

CIPROFLOXACIN; FLUORESCENT DYE;

AMPLICON; ANALYTIC METHOD; ANTIBIOTIC RESISTANCE; ARTICLE; BACTERIAL GENE; BACTERIAL GENETICS; BACTERIAL STRAIN; BACTERIUM IDENTIFICATION; BACTERIUM ISOLATION; BIOASSAY; CAMPYLOBACTER JEJUNI; CODON; CONTROLLED STUDY; FLUORESCENCE RESONANCE ENERGY TRANSFER; GENE MUTATION; GENETIC ANALYSIS; GYRA GENE; MUTATIONAL ANALYSIS; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; REAL TIME POLYMERASE CHAIN REACTION; REPRODUCIBILITY;

BASE SEQUENCE; CAMPYLOBACTER JEJUNI; CIPROFLOXACIN; DNA GYRASE; DRUG RESISTANCE, MICROBIAL; FLUORESCENCE RESONANCE ENERGY TRANSFER; FLUORESCENT DYES; GENE EXPRESSION REGULATION, BACTERIAL; MOLECULAR SEQUENCE DATA; POINT MUTATION;

CAMPYLOBACTER; CAMPYLOBACTER JEJUNI;

EID: 3242683434     PISSN: 08908508     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.mcp.2004.02.001     Document Type: Article

References (25)

1. Tauxe R.V. Epidemiology of Campylobacter jejuni infections in the United States and other industrialized nations. Nachamkin I., Blaser M.J., Tompkins L.S. Campylobacter jejuni: current status and future trends. 1992;9 American Society for Microbiology, Washington, DC
2. Jacobs-Reitsma W.F. Aspects of epidemiology of Campylobacter in poultry. Vet Q. 19:1997;113-117
3. Pezzotti G., Serafin A., Luzzi I., Mioni R., Milan M., Perin R. Occurrence and antimicrobial resistance of Campylobacter jejuni and Campylobacter coli in animals and meat in north-eastern Italy. Int J Food Microbiol. 82:2002;281-287
4. Balfour J.A., Faulds D. Oral ciprofloxacin: a pharmacoeconomic evaluation of its use in the treatment of serious infections. Pharmacoeconomics. 3:1993;398-421
5. Engberg J., Aarestrup F.M., Taylor D.E., Gerner-Smidt P., Nachamkin I. Quinolone and macrolide resistance in Campylobacter jejuni and C. coli: resistance mechanisms and trends in human isolates. Emerg Infect Dis. 7:2001;24-34
6. Piddock L.J.V. Quinolone resistance and Campylobacter. Clin Microbiol Infect. 5:1999;239-243
7. Charvalos E., Peteinaki E., Spyridaki I., Manetas S., Tselentis Y. Detection of ciprofloxacin resistance mutations in Campylobacter jejuni gyrA by non-radioisotopic single-strand conformation polymorphism and direct DNA sequencing. J Clin Lab Anal. 10:1996;129-133
8. Gibreel A., Sjogren E., Kaijser B., Wretlind B., Skold O. Rapid emergence of high-level resistance to quinolones in Campylobacter jejuni associated with mutational changes in gyrA and parC. Antimicrob Agents Chemother. 42:1998;3276-3278
9. Husmann M., Feddersen A., Steits A., Freytag C., Bhakdi S. Simultaneous identification of Campylobacters and prediction of quinolone resistance by comparative sequence analysis. J Clin Microbiol. 35:1997;2398-2400
10. Ruiz J., Goni P., Marco F., Gallardo F., Mirelis B., De Anta D.J., Vila J. Increased resistance to quinolones in Campylobacter jejuni: a genetic analysis of gyrA gene mutations in quinolone-resistant clinical isolates. Microbiol Immunol. 42:1998;223-226
11. Yoshida H., Bogaki M., Nakamura M., Nakamura S. Quinolone resistance-determining region in the DNA gyrase gyrA gene of Escherichia coli. Antimicrob Agents Chemother. 34:1990;1271-1272
12. Wang Y., Huang W.M., Taylor D.E. Cloning and nucleotide sequence of the Campylobacter jejuni gyrA and characterization of quinolone resistance mutations. Antimicrob Agents Chemother. 37:1993;457-463
13. Walker R.A., Saunders N., Lawson A.J., Lindsay E.A., Dassama M., Ward L.R., Woodward M.J., Davies R.H., Liebana E., Threlfall E.J. Use of a LightCycler gyrA mutation assay for rapid identification of mutations conferring decreased susceptibility to ciprofloxacin in multiresistant Salmonella enterica serotype Typhimurium DT104 isolates. J Clin Microbiol. 39:2001;1443-1448
14. Carattoli A., Dionisi A., Luzzi I. Use of a LightCycler gyrA mutation assay for identification of ciprofloxacin-resistant Campylobacter coli. FEMS Microbiol Lett. 214:(1):2002;87-93
15. Caplin B.E., Rasmussen R.P., Bernard P.S., Wittwer C.T. LightCycler hybridization probes: the most direct way to monitor PCR amplification for quantitation and mutation detection. Biochemica. 1:1999;5-9
16. Lennette EH, Balows A, Hausler Jr WJ, Shadomy HJ. Manual of clinical microbiology, 4th ed. 1885, American society for microbiology, Washington, DC.
17. Baker C.N. The E-Test and Campylobacter jejuni. Diagn Microbiol Infect Dis. 15:(5):1992;469-472
18. Zirnstein G., Li Y., Swaminathan B., Angulo F. Ciprofloxacin resistance in Campylobacter jejuni isolates: detection of gyrA resistance mutations by mismatch amplification mutation assay PCR and DNA sequence analysis. J Clin Microbiol. 37:1999;3276-3280
19. Sanger F., Nicklen S., Coulson A.R. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci USA. 74:1977;5463-5467
20. Piddock L.J., Ricci V., Pumbwe L., Everett M.J., Griggs D.J. Fluoroquinolone resistance in Campylobacter species from man and animals: detection of mutations in topoisomerase genes. J Antimicrob Chemother. 51:(1):2003;19-26
21. Charvalos E., Tselentis Y., Hamzehpour M.M., Kohler T., Pecher J.C. Evidence for efflux pump in multidrug resistant C. jejuni. Antimicrob Agents Chemother. 39:1995;2019-2022
22. Pumbwe L., Piddock L.J. Identification and molecular characterisation of CmeB, a Campylobacter jejuni multidrug efflux pump. FEMS Microbiol Lett. 206:(2):2002;185-189
23. Hakanen A., Jalava J., Kotilainen P., Jousimies-Somer H., Siitonen A., Huovinen P. gyrA polymorphism in Campylobacter jejuni: detection of gyrA mutations in 162 C. jejuni isolates by single-strand conformation polymorphism and DNA sequencing. Antimicrob Agents Chemother. 46:(8):2002;2644-2647
24. Ge B., Bodeis S., Walker R.D., White D.G., Zhao S., McDermott P.F., Meng J. Comparison of the E-test and agar dilution for in vitro antimicrobial susceptibility testing of Campylobacter. J Antimicrob Chemother. 50:(4):2002;487-494
25. Wilson D.L., Abner S.R., Newman T.C., Mansfield L.S., Linz J.E. Identification of ciprofloxacin-resistant Campylobacter jejuni by use of a fluorogenic PCR assay. J Clin Microbiol. 38:2000;3971-3978