Integrated nanoreactor systems: Triggering the release and mixing of compounds inside single vesicles

Journal of the American Chemical Society

Volumn 126, Issue 28, 2004, Pages 8594-8595

  Bolinger, Pierre Yves ^a   Stamou, Dimitrios ^a   Vogel, Horst ^a  

^a EPFL   (Switzerland)

Author keywords

[No Author keywords available]

Indexed keywords

HYDROCARBON; LIPID;

ARTICLE; CHEMICAL REACTION; CONCENTRATION (PARAMETERS); LIPID COMPOSITION; PHASE TRANSITION; PROTEIN EXPRESSION; REACTION ANALYSIS; REACTOR; TEMPERATURE DEPENDENCE;

LIPID BILAYERS; LIPOSOMES; MODELS, MOLECULAR; NANOTECHNOLOGY; PARTICLE SIZE; PERMEABILITY; TEMPERATURE;

EID: 3242669525     PISSN: 00027863     EISSN: None     Source Type: Journal    
DOI: 10.1021/ja049023u     Document Type: Article

References (22)

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13. 2000]). SUVs are made of DPPC/DPPG at a molar ratio of 9:1. LUVs are made of DOPG/rhodamine-PE/biotin-PEG-lipid, molar ratio 96:1:3.
14. SUVs are hydrated with 15 mM CF (Sigma-Aldrich) and 200 mM sorbitol (Fluka). After 5 freeze-thaw cycles, the vesicle solution is extruded (Lipex Biomembrane, Inc.) eight times through filters of 400 nm pore diameter at 70 °C. The untrapped dye is removed by gel exclusion chromatography (Sephadex G-75) at room temperature, eluting with a solution of 10 mM KCl and 200 mM sorbitol. LUVs are prepared by hydrating with the solution of SUVs at room temperature for 2 days.
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16. 4 and 200 mM sorbitol, pH 7.4. After being rinsed with buffer, the neutravidin surface was incubated for 5 min with a solution of LUVs and then washed again. The immobilized LUVs were imaged by laser scanning confocal fluorescence microscopy. Excitation at 488 nm, emission at LP 505 nm. The temperature of the solution was regulated from 20 to 50 °C with two Peltier elements; control and recording is implemented in LabVIEW (National Instruments).
17. If desired, dialysis can be used to create an approximate lower limit to the size distribution.18 upper limit can be defined by gentle filtration through 5-10 μm pores, data not shown.
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