An alkaline solution simplifies nucleic acid preparation for RT-PCR and infectivity assays of viroids from crude sap and spotted membrane

Journal of Virological Methods

Volumn 132, Issue 1-2, 2006, Pages 204-211

  Singh, Rudra P ^a   Dilworth, Andrea D ^a   Singh, Mathuresh ^b   Babcock, Krista M ^b  

^a FREDERICTON RESEARCH AND DEVELOPMENT CENTRE   (Canada)

^b Agricultural Certification Services   (Canada)

Author keywords

Long distance transportation; Nucleic acid extraction; Ornamental plants; Phenolics; Potato tubers; Tomato leaves

Indexed keywords

ALKALI; EDETIC ACID; NUCLEIC ACID; PYROXYLIN; SODIUM HYDROXIDE; WATER;

ARTICLE; BIOASSAY; CENTRIFUGATION; NONHUMAN; PLANT; POTATO; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; ROOM TEMPERATURE; SUPERNATANT; TOMATO; VACUUM; VIROID; VIRUS DETECTION;

ADSORPTION; COLLODION; HYDROGEN-ION CONCENTRATION; LYCOPERSICON ESCULENTUM; MICROPORE FILTERS; PLANT DISEASES; PLANT LEAVES; PLANT TUBERS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, VIRAL; SOLANUM TUBEROSUM; VIROIDS;

LYCOPERSICON ESCULENTUM; SOLANUM TUBEROSUM; VIROIDS;

EID: 32344444071     PISSN: 01660934     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jviromet.2005.09.007     Document Type: Article

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