A reliable RT-PCR-ELISA method for the detection of infectious pancreatic necrosis virus (IPNV) in farmed rainbow trout

Journal of Virological Methods

Volumn 132, Issue 1-2, 2006, Pages 92-96

  Milne, S A ^a   Gallacher, S ^b   Cash, P ^a   Porter, A J R ^a  

^a UNIVERSITY OF ABERDEEN   (United Kingdom)

^b MARINE LABORATORY   (United Kingdom)

Author keywords

Detection; ELISA; IPNV; RT PCR

Indexed keywords

ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; CONTAMINATION; CONTROLLED STUDY; ENZYME LINKED IMMUNOSORBENT ASSAY; INFECTIOUS PANCREATIC NECROSIS VIRUS; KIDNEY PARENCHYMA; NONHUMAN; PISCICULTURE; PRIORITY JOURNAL; RAINBOW TROUT; RELIABILITY; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SAFETY; SAMPLING; SCREENING; STANDARDIZATION; VIRUS DETECTION; VIRUS INFECTION;

ANIMALS; BIRNAVIRIDAE INFECTIONS; ENZYME-LINKED IMMUNOSORBENT ASSAY; FISH DISEASES; INFECTIOUS PANCREATIC NECROSIS VIRUS; KIDNEY; ONCORHYNCHUS MYKISS; REPRODUCIBILITY OF RESULTS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY;

ANIMALIA; INFECTIOUS PANCREATIC NECROSIS VIRUS; ONCORHYNCHUS MYKISS; SALMONIDAE;

EID: 32344439097     PISSN: 01660934     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jviromet.2005.09.003     Document Type: Article

References (18)

1. M. Alonso, I. Cano, D. Castro, S.I. Perez-Prieto, and J.J. Borrego Development of an in situ hybridisation procedure for the detection of sole aquabirnavirus in infected fish cell cultures J. Virol. Methods 116 2004 133 138
2. M. Alonso, S. Rodriguez, and S.I. Perez-Prieto Nested PCR improves detection of infectious hematopoietic necrosis virus in cells co-infected with infectious pancreatic necrosis virus J. Virol. Methods 81 1999 1 9
3. K.H. Amos, K.A. Hopper, and L. LeVander Absence of infectious hematopoietic necrosis virus in adult sockeye salmon J. Aquat. Anim. Health 1 1989 281 283
4. S.L. Blake, W.B. Schill, P.E. McAllister, M.K. Lee, and J.T. Singer Detection and identification of aquatic birnaviruses by PCR assay J. Clin. Micro. 33 1995 835 839
5. M. Deng, S.P. Day, and D.O. Cliver Detection of hepatitis A virus in environmental samples by antigen-capture PCR Appl. Environ. Microbiol. 60 1994 1927 1933
6. P. Dobos, B.J. Hill, R. Hallett, D.T.C. Kells, H. Becht, and D. Teninges Biophysical and biochemical characterization of five animal viruses with bisegmented double-stranded RNA genome J. Virol. 32 1979 593 605
7. J.C. Espinoza, and J. Kuznar Rapid simultaneous detection and quantitation of infectious pancreatic necrosis virus (IPNV) J. Virol. Methods 105 2002 81 85
8. D. Häfliger, M. Gilgen, J. Lüthy, and Ph. Hübner Seminested RT-PCR systems for small round structured viruses and detection of enteric viruses in seafood Int. J. Food Microbiol. 37 1997 27 36
9. B.J. Hill Infectious pancreatic necrosis virus and its virulence R.J. Roberts Microbial Diseases of Fish 1982 Academic Press New York 91 114
10. D.P. McGregor, P.E. Molloy, C. Cunningham, and W.J. Harris Spontaneous assembly of a bivalent single chain antibody fragment in Eschericia coli Mol. Immunol. 31 1994 215 226
11. Milne, S.A., Gallacher, S., Cash, P., Henshilwood, K., Lees, D., Porter, A.J.R. Development of a sensitive and reliable molecular method for the detection of human pathogenic viruses in bivalve molluscs, submitted for publication.
12. A. Pryde, W.T. Melvin, and A.L.S. Munro Nucleotide sequence analysis of the serotype-specific epitope of infectious pancreatic necrosis virus Arch. Virol. 129 1993 287 293
13. S. Rodriguez, J.J. Borrego, and S.I. Perez-Prieto Comparitive evaluation of five serological methods and RT-PCR assay for the detection of IPNV in fish J. Virol. Methods 97 2001 23 31
14. K.J. Schwab, R. Leon, and M.D. Sobsey Immunoaffinity concentration and purification of waterborne enteric viruses for detection by reverse transcriptase PCR Appl. Environ. Microbiol. 62 1996 2086 2094
15. S. Shen, U. Desselberger, and T.A. McKee The development of an antigen capture polymerase chain reaction assay to detect and type human enteroviruses J. Virol. Methods 65 1997 139 144
16. K. Soo Jin, O. Hae Keun, and C. Tae-Jin Detection of fish virus by using immunomagnetic separation and polymerase chain reaction (IMS-PCR) J. Korean Fish. Soc. 30 1997 948 955
17. E. Suñén, and M.D. Sobsey Recovery and detection of enterovirus, hepatitis A virus and Norwalk virus in hardshell clams (Mercenaria mercenaria) by RT-PCR methods J. Virol. Methods 77 1999 179 187
18. K. Williams, S. Blake, A. Sweeney, J.T. Singer, and B.L. Nicholson Mutliplex reverse transcriptase PCR assay for simultaneous detection of three fish viruses J. Clin. Microbiol. 37 1999 4139 4141