Fluorescence in situ hybridization with specific oligonucleotide rRNA probes distinguishes the sibling species Stylonychia lemnae and Stylonychia mytilus (Ciliophora, Spirotrichea)

Protist

Volumn 157, Issue 1, 2006, Pages 21-30

  Schmidt, Stephanie L ^a   Bernhard, Detlef ^a   Schlegel, Martin ^a   Fried, Johannes ^b  

^a UNIVERSITY OF LEIPZIG   (Germany)

^b TECHNICAL UNIVERSITY OF MUNICH   (Germany)

Author keywords

Ciliophora; Gene probes; Identification; In situ hybridization; Small subunit rRNA; Stylonychia mytilus complex

Indexed keywords

CILIOPHORA; PROTOZOA; SPIROTRICHEA; STYLONYCHIA LEMNAE; STYLONYCHIA MYTILUS;

EID: 32344435348     PISSN: 14344610     EISSN: 16180941     Source Type: Journal    
DOI: 10.1016/j.protis.2005.09.004     Document Type: Article

References (37)

1. Amann RI (1995) In Situ Identification of Micro-Organisms by Whole Cell Hybridization with rRNA-targeted Nucleic Acid Probes. In Akkeman ADC, van Elsas JD, de Bruigin FJ (eds) Molecular Microbial Ecology Manual, 3.3.6, Kluwer Academic Publishers, Dordrecht, pp 1-15
2. R.I. Amann, W. Ludwig, and K.H. Schleifer Phylogenetic identification and in situ detection of individual microbial cells without cultivation Microbiol Rev 59 1995 143 169
3. D. Ammermann Cytologische und genetische Untersuchungen an dem Ciliaten Stylonychia mytilus Ehrenberg Arch Protistenkd 108 1965 109 152
4. D. Ammermann, and M. Schlegel Characterization of two sibling species of the genus Stylonychia (Ciliata, Hypotricha): S. mytilus Ehrenberg, 1838 and S. lemnae n. sp. I. Morphology and reproductive behavior J Protozool 30 1983 290 294
5. T. Behr, C. Koob, M. Schedl, A. Mehlen, H. Meier, D. Knopp, E. Frahm, U. Obst, K. Schleifer, R. Niessner, and W. Ludwig A nested array of rRNA targeted probes for the detection and identification of enterococci by reverse hybridization Syst Appl Microbiol 23 2000 563 572
6. H. Berger Monograph of the Oxytrichidae (Ciliophora, Hypotrichia) 1999 Kluwer Academic Publishers Dordrecht
7. H. Berger, and W. Foissner Cladistic relationships and generic characterization of oxytrichid hypotrichs (Protozoa, Ciliophora) Arch Protistenkd 148 1997 125 155
8. D. Bernhard, A. Stechmann, W. Foissner, D. Ammermann, M. Hehn, and M. Schlegel Phylogenetic relationships within the class Spirotrichea (Ciliophora) inferred from small subunit rRNA gene sequences Mol Phylogenet Evol 21 2001 86 92
9. D. Borden, E.T. Miller, G.S. Whitt, and D.L. Nanney Electrophoretic analysis of evolutionary relationships in Tetrahymena Evolution 31 1977 91 102
10. A.W. Coleman Paramecium aurelia revisited J Eukaryot Microbiol 52 2005 68 77
11. H.J. Elwood, G.J. Olsen, and M.L. Sogin The small-subunit ribosomal RNA gene sequences from the hypotrichous ciliates Oxytricha nova and Stylonychia pustulata Mol Biol Evol 2 1985 399 410
12. W. Foissner, H. Berger, and F. Kohmann Taxonomische und ökologische Revision der Ciliaten des Saprobiensystems - Band II: Peritrichia, Heterotrichida, Odontostomatida Informationsberichte des Bayer. Landesamtes für Wasserwirtschaft 1992 Bartels und Wernitz Druck Munich, Germany
13. W. Foissner, S.Y. Moon-van der Staay, G.W.M. van der Staay, J.H.P. Hackstein, W-.D. Krautgartner, and H. Berger Reconciling classical and molecular phylogenies in the stichotrichines (Ciliophora, Spirotrichea), including new sequences from some rare species Eur J Protistol 40 2004 265 281
14. J. Fried, W. Ludwig, R. Psenner, and K.H. Schleifer Improvement of ciliate identification and quantification: a new protocol for fluorescence in situ hybridization (FISH) in combination with silver stain techniques Syst Appl Microbiol 25 2002 555 571
15. S.J. Giovannoni, E.F. DeLong, G.J. Olson, and N.R. Pace Phylogenetic group-specific oligodeoxynucleotide probes for identification of single microbial cells J Bacteriol 170 1988 720 726
16. D. Grimm, H. Merkert, W. Ludwig, K.H. Schleifer, J. Hacker, and B.C. Brand Specific detection of Legionella pneumophila: construction of a new 16S rRNA-targeted oligonucleotide probe Appl Environ Microbiol 64 1998 2686 2690
17. D.F. Gruchy The breeding system and distribution of Tetrahymena pyriformis J Protozool 2 1955 178 185
18. R. Gupta, K. Kamra, S. Arora, and G.R. Sapra Stylonychia ammermanni sp. n., a new oxytrichid (Ciliophora: Hypotrichida) ciliate from the river Yamuna, Delhi, India Acta Protozool 40 2001 75 82
19. Häntzsch M, Schmidt SL, Bernhard D, Ammermann D, Schlegel M (2004) Entwicklung von spezifischen DNA-Sonden bei Ciliaten. 3rd Leipzig Research Festival for Life Sciences, vol. 254. p. 271
20. E.A. Hewitt, K.M. Müller, J. Cannone, D.J. Hogan, R. Gutell, and D.M. Prescott Phylogenetic relationships among 28 spirotrichous ciliates documented by rDNA Mol Phylogenet Evol 29 2003 258 267
21. W. Ludwig, O. Strunk, R. Westram, L. Richter, H. Meier, Yadhukumar, A. Buchner, T. Lai, S. Steppi, G. Jobb, W. Förster, I. Brettske, S. Gerber, A.W. Ginhart, O. Gross, S. Grumann, S. Hermann, R. Jost, A. König, T. Liss, R. Lüßmann, M. May, B. Nonhoff, B. Reichel, R. Strehlow, A. Stamatakis, N. Stuckmann, A. Vilbig, M. Lenke, T. Ludwig, A. Bode, and K.H. Schleifer ARB: a software environment for sequence data Nucleic Acids Res 32 2004 1363 1371
22. D.H. Lynn Morphology or molecules: How do we identify the major lineages of ciliates (Phylum Ciliophora)? Eur J Protistol 39 2003 356 364
23. W. Manz, U. Szewzyk, P. Ericsson, R. Amann, K.H. Schleifer, and T.A. Stenstrom In situ identification of bacteria in drinking water and adjoining biofilms by hybridization with 16S and 23S rRNA-directed fluorescent oligonucleotide probes Appl Environ Microbiol 59 1993 2293 2298
24. D.L. Nanney, L.E. Cooper, E.M. Simon, and G.S. Whitt Isozymic characterization of three mating groups of the Tetrahymena pyriformis complex J Protozool 27 1980 451 458
25. G. Petroni, F. Dini, F. Verni, and G. Rosati A molecular approach to the tangled intrageneric relationships underlying phylogeny in Euplotes (Ciliophora, Spirotrichea) Mol Phylogenet Evol 22 2002 118 130
26. G. Petroni, G. Rosati, C. Vannini, L. Modeo, F. Dini, and F. Verni In situ identification by fluorescently labeled oligonucleotide probes of morphologically similar, closely related ciliate species Microb Ecol 45 2003 156 162
27. M. Regensbogenova, S. Kisidayova, T. Michalowski, P. Javorsky, S.Y. Moon-van der Staay, G.W.M. Moon-van der Staay, J.H.P. Hackstein, N.R. McEwan, J.-P. Jouany, J.C. Newbold, and P. Pristas Rapid identification of rumen protozoa by restriction analysis of amplified 18S rRNA gene Acta Protozool 43 2004 219 224
28. J. Rice, C.D. O'Connor, M.A. Sleigh, P.H. Burkhill, I.G. Giles, and M.V. Zubkov Fluorescent oligonucleotide rDNA probes that specifically bind to a common nanoflagellate, Paraphysomonas vestita Microbiology 143 1997 1717 1727
29. M. Schlegel, and R. Meisterfeld The species problem in protozoa revisited Eur J Protistol 39 2003 349 355
30. M. Schlegel, H.J. Elwood, and M.L. Sogin Molecular evolution in hypotrichous ciliates: sequence of the small subunit ribosomal RNA genes from Onychodromus quadricornutus and Oxytricha granulifera (Oxytrichidae, Hypotrichida, Ciliophora) J Mol Evol 32 1991 64 69
31. X.-B. Shi, and D. Ammermann Stylonychia harbinensis sp. n., a new oxytrichid ciliate (Ciliophora, Hypotrichia) from the Heilongjiang Province, China Protistology 3 2004 219 222
32. Sonneborn TM (1957) Breeding Systems, Reproductive Methods and Species Problems in Protozoa. In Mayr E (ed) The Species Problem. AAA Symposium. American Association of Advanced Sciences, Washington, DC, pp 155-324
33. G. Steinbrück, and M. Schlegel Characterization of two sibling species of the genus Stylonychia (Ciliata, Hypotrichia): S. mytilus Ehrenberg, 1838 and S. Lemnae n. sp. II. Biochemical characterization J Protozool 30 1983 294 300
34. T. Stoeck, W.H. Fowle, and S.S. Epstein Methodology of protistian discovery: from rRNA detection to quality scanning electron microscope images Appl Environ Microbiol 69 2003 6856 6863
35. T. Stoeck, H. Welter, D. Seitz-Bender, J. Kusch, and H.J. Schmidt ARDRA and RAPD-fingerprinting reject the sibling species concept for the ciliate Paramecium caudatum (Ciliophora, Protoctista) Zool Scri 29 2000 75 82
36. T. Weisse, H. Müller, R.M. Pinto-Coelho, A. Schweizer, D. Springmann, and G. Baldringer Response of the microbial loop to the phytoplankton spring bloom in a large prealpine lake Limnol Oceanogr 35 1990 781 794
37. E. Wirnsberger, W. Foissner, and H. Adam Biometric and morphogenetic comparison of the sibling species Stylonychia mytilus and S. lemnae, including a phylogenetic system for the oxytrichids (Ciliophora, Hypotrichida) Arch Protistenkd 132 1986 167 185