Construction and evaluation of a chromosomal expression platform (CEP) for ectopic, maltose-driven gene expression in Streptococcus pneumoniae

Microbiology

Volumn 152, Issue 2, 2006, Pages 343-349

  Guiral, Sébastien ^a   Hénard, Vincent ^a   Laaberki, Maria Halima ^a,b   Granadel, Chantal ^a   Prudhomme, Marc ^a   Martin, Bernard ^a   Claverys, Jean Pierre ^a  

^a UPS   (France)

^b UNIVERSITÉ DE TOULOUSE   (France)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL DNA; KANAMYCIN; MALTOSE;

ARTICLE; BACTERIAL CHROMOSOME; BACTERIUM TRANSFORMATION; CHROMOSOME ANALYSIS; CONTROLLED STUDY; DNA DETERMINATION; ESCHERICHIA COLI; GENE CASSETTE; GENE EXPRESSION REGULATION; MOLECULAR CLONING; NONHUMAN; PLASMID; PRIORITY JOURNAL; STREPTOCOCCUS PNEUMONIAE;

BACTERIOLOGICAL TECHNIQUES; CHROMOSOMES, BACTERIAL; CROSSES, GENETIC; GENE EXPRESSION REGULATION, BACTERIAL; MALTOSE; STREPTOCOCCUS PNEUMONIAE;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; STREPTOCOCCUS PNEUMONIAE;

EID: 32244446796     PISSN: 13500872     EISSN: None     Source Type: Journal    
DOI: 10.1099/mic.0.28433-0     Document Type: Article

References (36)

1. Alloing, G., Trombe, M. C. & Claverys, J. P. (1990). The ami locus of the Gram-positive bacterium Streptococcus pneumoniae is similar to binding protein-dependent transport operons of Gram-negative bacteria. Mol Microbiol 4, 633-644.
2. Balganesh, T. S. & Lacks, S. A. (1985). Heteroduplex DNA mismatch repair system of Streptococcus pneumoniae: cloning and expression of the hexA gene. J Bacteriol 162, 979-984.
3. Bergé, M., Moscoso, M., Prudhomme, M., Martin, B. & Claverys, J. P. (2002). Uptake of transforming DNA in Gram-positive bacteria: a view from Streptococcus pneumoniae. Mol Microbiol 45, 411-421.
4. Chan, P. F., O'Dwyer, K. M., Palmer, L. M. & 8 other authors (2003). Characterization of a novel fucose-regulated promoter (PfcsK) suitable for gene essentiality and antibacterial mode-of-action studies in Streptococcus pneumoniae. J Bacteriol 185, 2051-2058.
5. Dagkessamanskaia, A., Moscoso, M., Hénard, V., Guiral, S., Overweg, K., Reuter, M., Martin, B., Wells, J. & Claverys, J. P. (2004). Interconnection of competence, stress and CiaR regulons in Streptococcus pneumoniae: competence triggers stationary phase autolysis of ciaR mutant cells. Mol Microbiol 51, 1071-1086.
6. de Saizieu, A., Gardes, C., Flint, N., Wagner, C., Kamber, M., Mitchell, T. J., Keck, W., Amrein, K. E. & Lange, R. (2000). Microarray-based identification of a novel Streptococcus pneumoniae regulon controlled by an autoinduced peptide. J Bacteriol 182, 4696-4703.
7. Díaz, A., Lacks, S. A. & López, P. (1992). The 5′ to 3′ exonuclease activity of DNA polymerase I is essential for Streptococcus pneumoniae. Mol Microbiol 6, 3009-3019.
8. Guiral, S., Hénard, V., Granadel, C., Martin, B. & Claverys, J. P. (2006). Inhibition of competence development in Streptococcus pneumoniae by increased basal-level expression of the ComDE two-component regulatory system. Microbiology, 152, 323-331.
9. Hoskins, J., Alborn, W. E., Jr, Arnold, J. & 40 other authors (2001). Genome of the bacterium Streptococcus pneumoniae strain R6. J Bacteriol 183, 5709-5717.
10. Kausmally, L., Johnsborg, O., Lunde, M., Knutsen, E. & Håvarstein, L. S. (2005). Choline-binding protein D (CbpD) in Streptococcus pneumoniae is essential for competence-induced cell lysis. J Bacteriol 187, 4338-4345.
11. Kong, L., Siranosian, K. J., Grossman, A. D. & Dubnau, D. (1993). Sequence and properties of mecA, a negative regulator of genetic competence in Bacillus subtilis. Mol Microbiol 9, 365-373.
12. Lacks, S. A. (1968). Genetic regulation of maltosaccharide utilization in pneumococcus. Genetics 60, 685-706.
13. Luo, P., Li, H. & Morrison, D. A. (2003). ComX is a unique link between multiple quorum sensing outputs and competence in Streptococcus pneumoniae. Mol Microbiol 50, 623-633.
14. Mannarelli, B. M. & Lacks, S. A. (1984). Ectopic integration of chromosomal genes in Streptococcus pneumoniae. J Bacteriol 160, 867-873.
15. Martin, B., Prats, H. & Claverys, J. P. (1985). Cloning of the hexA mismatch repair of Streptococcus pneumoniae and identification of the product. Gene 34, 293-303.
16. Martin, B., Alloing, G., Boucraut, C. & Claverys, J. P. (1989). The difficulty of cloning Streptococcus pneumoniae mal and ami loci in Escherichia coli: toxicity of malX and amiA gene products. Gene 80, 227-237.
17. Martin, B., Prudhomme, M., Alloing, G., Granadel, C. & Claverys, J. P. (2000). Cross-regulation of competence pheromone production and export in the early control of transformation in Streptococcus pneumoniae. Mol Microbiol 38, 867-878.
18. Méjean, V., Claverys, J. P., Vasseghi, H. & Sicard, A. M. (1981). Rapid cloning of specific DNA fragments of Streptococcus pneumoniae by vector integration into the chromosome followed by endonucleolytic excision. Gene 15, 289-293.
19. Mortier-Barrière, I., de Saizieu, A., Claverys, J. P. & Martin, B. (1998). Competence-specific induction of recA is required for full recombination proficiency during transformation in Streptococcus pneumoniae. Mol Microbiol 27, 159-170.
20. Nieto, C., Espinosa, M. & Puyet, A. (1997). The maltose/maltodextrin regulon of Streptococcus pneumoniae. J Biol Chem 272, 30860-30865.
21. Nieto, C., Fernández de Palencia, P., López, P. & Espinosa, M. (2000). Construction of a tightly regulated plasmid vector for Streptococcus pneumoniae: controlled expression of the green fluorescent protein. Plasmid 43, 205-213.
22. Peterson, S., Sung, C. K., Cline, R. & 13 other authors (2004). Identification of competence pheromone responsive genes in Streptococcus pneumoniae. Mol Microbiol 51, 1051-1070.
23. Pichoff, S., Vollrath, B., Touriol, C. & Bouché, J. P. (1995). Deletion analysis of gene minE which encodes the topological specificity factor of cell division in Escherichia coli. Mol Microbiol 18, 321-329.
24. Pozzi, G. & Guild, W. R. (1985). Modes of integration of heterologous plasmid DNA into the chromosome of Streptococcus pneumoniae. J Bacteriol 161, 909-912.
25. Prats, H., Martin, B. & Claverys, J. P. (1985). The hexB mismatch repair gene of Streptococcus pneumoniae: characterization, cloning and identification of the product. Mol Gen Genet 200, 482-489.
26. Prudhomme, M. & Claverys, J. P. (2006). There will be a light: the use of luc transcriptional fusions in living pneumococcal cells. In The Molecular Biology of Streptococci. Edited by R. Hakenbeck & G. S. Chhatwal. Norwich, UK: Horizon Scientific Press.
27. Puyet, A., Ibáñez, A. M. & Espinosa, M. (1993). Characterization of the Streptococcus pneumoniae maltosaccharide regulator MalR, a member of the LacI-GalR family of repressors displaying distinctive genetic features. J Biol Chem 268, 25402-25408.
28. Robertson, G. T., Ng, W. L., Foley, J., Gilmour, R. & Winkler, M. E. (2002). Global transcriptional analysis of clpP mutations of type 2 Streptococcus pneumoniae and their effects on physiology and virulence. J Bacteriol 184, 3508-3520.
29. Robertson, G. T., Ng, W. L., Gilmour, R. & Winkler, M. E. (2003). Essentiality of clpX, but not clpP, clpL, clpC, or clpE, in Streptococcus pneumoniae R6. J Bacteriol 185, 2961-2966.
30. Sambrook, J., Fritsch, E. F. & Maniatis, T. (1989). Molecular Cloning: a Laboratory Manual, 2nd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory.
31. Stassi, D. L., Dunn, J. J. & Lacks, S. A. (1982). Nucleotide sequence of DNA controlling expression of genes for maltosaccharide utilization in Streptococcus pneumoniae. Gene 20, 359-366.
32. Stieger, M., Wohlgensinger, B., Kamber, M., Lutz, R. & Keck, W. (1999). Integrational plasmids for the tetracycline-regulated expression of genes in Streptococcus pneumoniae. Gene 226, 243-251.
33. Sung, C. K., Li, H., Claverys, J. P. & Morrison, D. A. (2001). An rpsL cassette, janus, for gene replacement through negative selection in Streptococcus pneumoniae. Appl Environ Microbiol 67, 5190-5196.
34. Vasseghi, H. & Claverys, J. P. (1983). Amplification of a chimeric plasmid carrying an erythromycin-resistance determinant introduced into the genome of Streptococcus pneumoniae. Gene 21, 285-292.
35. Vasseghi, H., Claverys, J. P. & Sicard, A. M. (1981). Mechanism of integrating foreign DNA during transformation of Streptococcus pneumoniae. In Transformation-1980, pp. 137-154. Edited by M. Polsinelli & G. Mazza. Oxford: Cotswold Press.
36. Zähner, D. & Hakenbeck, R. (2000). The Streptococcus pneumoniae beta-galactosidase is a surface protein. J Bacteriol 182, 5919-5921.