An improved, real-time PCR assay for the detection of GC-rich and low abundance templates of Mycobacterium tuberculosis

Journal of Microbiological Methods

Volumn 64, Issue 3, 2006, Pages 406-410

  Grassi, Manuela ^a   Volpe, Elisabetta ^a,b   Colizzi, Vittorio ^b   Mariani, Francesca ^a  

^a CNR   (Italy)

^b UNIVERSITY OF ROME TOR VERGATA   (Italy)

Author keywords

Co solvents; GC rich transcripts; Intracellular pathogen; Quantitation; Specificity

Indexed keywords

BACTERIAL DNA; BACTERIAL RNA; GUANINE NUCLEOTIDE; MESSENGER RNA; PYRIMIDINE NUCLEOTIDE; SOLVENT;

ARTICLE; DNA TEMPLATE; MYCOBACTERIUM TUBERCULOSIS; NONHUMAN; PRIORITY JOURNAL; PROCESS OPTIMIZATION; QUANTITATIVE ANALYSIS; REAL TIME POLYMERASE CHAIN REACTION; RNA ANALYSIS; SAMPLE; SCALE UP; SENSITIVITY AND SPECIFICITY; TEMPERATURE DEPENDENCE;

BASE COMPOSITION; FLUORESCENT DYES; FORMAMIDES; HUMANS; MYCOBACTERIUM TUBERCULOSIS; ORGANIC CHEMICALS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, MESSENGER; SENSITIVITY AND SPECIFICITY; TEMPLATES, GENETIC; TUBERCULOSIS;

BACTERIA (MICROORGANISMS); MYCOBACTERIUM TUBERCULOSIS;

EID: 32244432868     PISSN: 01677012     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.mimet.2005.05.002     Document Type: Article

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