Ca-Mg phosphate nano-crystals for high efficiency gene delivery and expression in mammalian cells

2005 NSTI Nanotechnology Conference and Trade Show - NSTI Nanotech 2005 Technical Proceedings

Volumn , Issue , 2005, Pages 284-287

  Chowdhury, E H ^a   Akaike, Toshihiro ^a  

^a TOKYO INSTITUTE OF TECHNOLOGY   (Japan)

Author keywords

Calcium phosphate; Gene delivery; Hydroxyapatite; Magnesium; Nano apatite; Transfection

Indexed keywords

CELLS; DNA; GROWTH KINETICS; HYDROXYAPATITE; MAGNESIUM PRINTING PLATES; PRECIPITATION (CHEMICAL);

GENE DELIVERY; MAMMALIAN CELLS; NANO-APATITE; TRANSFECTION;

CALCIUM COMPOUNDS;

EID: 32044468472     PISSN: None     EISSN: None     Source Type: Conference Proceeding    
DOI: None     Document Type: Conference Paper

References (7)

1. Luo, D. & Saltzman, W. M. Synthetic DNA delivery systems. Nature Biotechnology 18, 33-37, 2000.
2. Fasbender, A., Lee, J.H., Walters, R.W., Moninger, T.O., Zabner, J. & Welsh, M.J. Incorporation of adenovirus in calcium phosphate precipitates enhances gene transfer to airway Epithelia in vitro and in vivo. J. Clin. Invest 102, 184-193, 1998.
3. Toyoda, K., Andresen, J., Zabner J., Faraci, F. & Heistad, D. Calcium phosphate precipitates augment adenovirus-mediated gene transfer to blood vessels in vitro and in vivo. Gene Therapy 7, 1284-1291, 2000.
4. Jordan, M., Schallhorn, A. & Wurm FM. Transfecting mammalian cells: optimization of critical parameters affecting calcium-phosphate precipitate formation. Nucleic acids research 24, 596-601, 1996.
5. Seelos, C. A critical parameter determining the aging of DNA-calcium-phosphate precipitates. Analytical Biochemistry 245, 109-111, 1996.
6. Chowdhury, et al. High-efficiency gene delivery for expression in mammalian cells by nanoprecipitates of Ca-Mg phosphate. Gene 341, 77-82, 2004.
7. Kibalczyc, W., Christoffersen, J., Christoffersen, M. R., Zielenkiewicz, A. and Zielenkiewicz, W. The effect of magnesium-ions on the precipitation of calcium phosphates. J. Cryst. Growth 106, 355-366, 1990.