Optimization and application of a multiplex RT-PCR system for simultaneous detection of five potato viruses using 18S rRNA as an internal control

Plant Disease

Volumn 90, Issue 2, 2006, Pages 185-189

  Du, ZhiYou ^a   Chen, JiShuang ^a,b   Hiruki, Chuji ^c  

^a ZHEJIANG UNIVERSITY   (China)

^b ZHEJIANG SCI TECH UNIVERSITY   (China)

^c UNIVERSITY OF ALBERTA   (Canada)

Author keywords

NADH hydrogenase subunit II; Tissue degradation

Indexed keywords

DEGRADATION; OPTIMIZATION; POLYMERS; RNA;

IMMUNOSORBENT ASSAY (DAS-ELISA); NADH HYDROGENASE SUBUNIT II; RNA MOLECULE; TISSUE DEGRADATION;

AGRICULTURAL PRODUCTS;

DEGRADATION; OPTIMIZATION; POLYMERS;

POTATO LEAFROLL VIRUS; POTATO VIRUS A; POTATO VIRUS S; POTATO VIRUS X; POTATO VIRUS Y; POTYVIRUS; SOLANUM TUBEROSUM;

EID: 32044463593     PISSN: 01912917     EISSN: None     Source Type: Journal    
DOI: 10.1094/PD-90-0185     Document Type: Article

References (23)

1. Bariana, H. S., Shannon, A. L., Chu, P. W. G., and Waterhouse, P. M. 1994. Detection of five seedborne legume viruses in one sensitive multiplex polymerase chain reaction test. Phytopathology 84:1201-1205.
2. Bertolini, E., Olmos, A., Martinez, M. C., Gorris, M. T., and Cambra, M. 2001. Single-step multiplex RT-PCR for simultaneous and colourimetric detection of six RNA viruses in olive trees. J. Virol. Methods 96:33-41.
3. Birch, D. E., Kolmondin, L., Laird, W. J., McKinney, N., Wong, J., Young, K. K. Y., Zangenberg, G. A., and Zoccoli, M. A. 1996. Amplified hot start PCR. Nature 381:445-446.
4. Boonham, N., Walsh, K., Smith, P., Madagan, K., Graham, I., and Barker, I. 2003. Detection of potato viruses using microarray technology: Towards a generic method for plant viral disease diagnosis. J. Virol. Methods 108:181-187.
5. Chamberlain, J. S., Gibbs, R. A., Ranier, J. E., Nguyen, P. N., and Caskey, C. T. 1988. Deletion screening of the Duchenne muscular dystrophy locus via multiplex DNA amplification. Nucleic Acid Res. 16:11141-11156.
6. Corne, J. M., Green, S., Sanderson, G., Caul, E. O., and Johnston, S. L. 1999. A multiplex RT-PCR for the detection of parainfluenza viruses 1-3 in clinical samples. J. Virol. Methods 82:9-18.
7. Elnifro, E. M., Ashshi, A. M., Cooper, R. J., and Klapper, P. E. 2002. Multiplex PCR: Optimization and Application in Diagnostic Virology. Clin. Microbiol. Rev. 13(4):559-570.
8. Hauser, S., Weber, C., Vetter, G., Stevens, M., Bwuve, M., and Lemaire, O. 2000. Improved detection and differentiation of poleroviruses infecting beet or rape by multiplex RT-PCR. J. Virol. Methods 89:11-21.
9. Jacobi, V., Bachand, G. D., Hamelin, R. C., and Castello, J. D. 1998. Development of a multiplex immunocapture RT-PCR assay for detection and differentiation of tomato and tobacco mosaic tobamoviruses. J. Virol. Methods 74:167-178.
10. Menzel, W., Jelkmann, W., and Maiss, E. 2002. Detection of four apple viruses by multiplex RT-PCR assays with coamplification of plant mRNA as internal control. J. Virol. Methods 99:81-92.
11. Nie, X., and Singh, R. P. 2000. Detection of multiple potato viruses using an oligo(dT) as a common cDNA primer in multiplex RT-PCR. J. Virol. Methods 86:179-185.
12. Nie, X., and Singh, R. P. 2001. A novel usage of random primers for multiplex RT-PCR detection of virus and viroid in aphids, leaves, and tubers. J. Virol. Methods 91:37-49.
13. Nie, X., and Singh, R. P. 2002. A new approach for the simultaneous differentiation of biological and geographical strains of potato virus Y by uniplex and multiplex RT-PCR. J. Virol. Methods 104:41-54.
14. Pang, W. F., and Wang, X. Y. 1997. Primary study on identification of potato virus in Hebei province, China. J. Hebei Agric. Sci. 1:19-23. (In Chinese)
15. Salazar, L. F., ed. 1996. Potato viruses and their control. Centro International de la Papa, Peru.
16. Singh, M., and Singh, R. P. 1997. Potato virus Y detection: Sensitivity of RT-PCR depends on the size of fragment amplified. Can. J. Plant Pathol. 19:149-155.
17. Singh, R. P. 1999. Development of the molecular methods for potato virus and viroid detection and prevention. Genome 42:592-604.
18. Smith, J., McElhinney, L. M., Heaton, P. R., Black, E. M., and Lowings, J. P. 2000. Assessment of template quality by the incorporation of an internal control into a RT-PCR for the detection of rabies and rabies-related viruses. J. Virol. Methods 84:107-115.
19. Thompson, J. R., Wetzel, S., Klerks, M. M., VǎSková, D., Schoen, C. D., Špak, J., and Jelkmann, W. 2003. Multiplex RT-PCR detection of four aphid-borne strawberry viruses in Fragaria spp. in combination with a plant mRNA specific internal control. J. Virol. Methods 11:85-93.
20. Wang, X. F., Liu, S. B., Wang, Y. F., Liu, W., and Jiang, L. 2002. Primary report on species and distribution of potato viruses in Dalian province, China. Liaoning Agric. Sci. 5:41-41. (In Chinese)
21. Zangenberg, G., Saiki, R. K., and Reynolds, R. 1999. Multiplex PCR: Optimization Guidelines. In: PCR Application: Protocols for Functional Genomics. M. A. Innis, D. H. Gelfand, and J. J. Sninsky, eds. Academic Press, San Diego.
22. Zhao, Z. J., He, Y. K., and Shui, Q. J. 2001. Progress of study on potato viruses in Yunnan province, China. J. Sinopotato 15:93-95. (In Chinese)
23. Zhou, X. P., Chen, J. S., Li, D. B., and Li, W. M. 1994. A method for high yield purification of Potyviruses. Microbiology 21(3):184-186. (In Chinese)