Specific and sensitive detection of Nosema bombi (Microsporidia: Nosematidae) in bumble bees (Bombus spp.; Hymenoptera: Apidae) by PCR of partial rRNA gene sequences

Journal of Invertebrate Pathology

Volumn 91, Issue 2, 2006, Pages 98-104

  Klee, Julia ^a   Tek Tay, Wee ^a,b   Paxton, Robert J ^a  

^a QUEEN'S UNIVERSITY BELFAST   (United Kingdom)

^b UNIVERSITY OF MELBOURNE   (Australia)

Author keywords

Apis mellifera; Bombus spp.; Diagnostic test; Nosema apis; Nosema bombi; Nosema ceranae; PCR; Ribosomal RNA gene

Indexed keywords

FUNGAL DNA;

ANIMAL; ARTICLE; BEE; DNA SEQUENCE; FUNGAL GENE; GENETICS; ISOLATION AND PURIFICATION; METHODOLOGY; MICROBIOLOGY; NOSEMA; POLYMERASE CHAIN REACTION; RNA GENE; SPECIES DIFFERENCE;

ANIMALS; BEES; DNA, FUNGAL; GENES, FUNGAL; GENES, RRNA; NOSEMA; POLYMERASE CHAIN REACTION; SEQUENCE ANALYSIS, DNA; SPECIES SPECIFICITY;

APIDAE; APINAE; APIS MELLIFERA; APOIDEA; BACTERIA (MICROORGANISMS); BOMBUS (GENUS); BOMBUS PENNSYLVANICUS; HYMENOPTERA; MICROSPORIDIA; NOSEMA; NOSEMA APIS; NOSEMA BOMBI; NOSEMA CERANAE; NOSEMATIDAE;

EID: 31944432355     PISSN: 00222011     EISSN: 10960805     Source Type: Journal    
DOI: 10.1016/j.jip.2005.10.012     Document Type: Article

References (29)

1. H.J. Banda, and R.J. Paxton Pollination of greenhouse tomatoes by bees Acta Hortic. 288 1991 194 199
2. S.A. Corbet, M. Fussell, R. Ake, A. Fraser, C. Gunson, A. Savage, and K. Smith Temperature and the pollinating activity of social bees Ecol. Entomol. 18 1993 17 30
3. H.B. Fantham, and A. Porter The morphology, biology and economic importance of Nosema bombi, parasitic in various humble bees (Bombus spp.) Ann. Trop. Med. Parasitol. 8 1914 623 638
4. Fries, I., Feng, F., 1995. Cross-infectivity of Nosema apis in Apis mellifera and Apis cerana. In: Proc. Apimondia 34th Int. Apic. Congress, Bucharest, Romania, pp. 151-155.
5. I. Fries, F. Feng, A.J. da Silva, S.B. Slemenda, and N.J. Pieniazek Nosema ceranae n. sp. (Microspora, Nosematidae), morphological and molecular characterization of a microsporidian parasite of the Asian honey bee Apis cerana (Hymenoptera, Apidae) Eur. J. Protistol. 32 1996 356 365
6. I. Fries, A. de Ruijter, R.J. Paxton, A.J. da Silva, S.B. Slemenda, and N.J. Pieniazek Molecular characterization of Nosema bombi (Microsporidia: Nosematidae) and a note on its sites of infection in Bombus terrestris (Hymenoptera: Apoidea) J. Apicult. Res. 40 2001 91 96
7. H.S. Gatehouse, and L.A. Malone The ribosomal RNA gene region of Nosema apis (microspora): DNA sequence for small and large subunit rRNA genes and evidence of a large tandem repeat unit size J. Invertebr. Pathol. 71 1998 97 105
8. K. Goka, K. Okabe, M. Yoneda, and S. Niwa Bumblebee commercialization will cause worldwide migration of parasitic mites Mol. Ecol. 10 2001 2095 2099
9. B. Imhoof, and P. Schmid-Hempel Colony success of the bumble bee, Bombus terrestris, in relation to infections by two protozoan parasites, Crithidia bombi and Nosema bombi Insect Soc. 46 1999 233 238
10. R. Larsson Ultrastructure, function and classification of Microsporidia Protistologica 1 1986 325 390
11. J. Leiro, R. Iglesias, A. Parama, W. Aragort, and M.L. Sanmartin PCR detection of Tetramicra brevifilum (Microspora) infection in turbot (Scophthalmus maximus L.) musculature Parasitology 124 2002 145 151
12. R.P. MacFarlane, J.J. Lipa, and H.J. Liu Bumble bee pathogens and internal enemies Bee World 76 1995 130 148
13. C.A. McIvor, and L.A. Malone Nosema bombi, a microsporidian pathogen of the bumble bee Bombus terrestris (L.) New Zeal. J. Zool. 22 1995 25 31
14. S. Niwa, H. Iwano, S. Asada, M. Matsuura, and K. Goka A microsporidian pathogen isolated from a colony of the European bumblebee, Bombus terrestris, and infectivity on Japanese bumblebee Jpn. J. Appl. Entomol. Zool. 48 2004 60 64
15. H. Rinder, K. Janitschke, H. Aspock, A.J. da Silva, P. Deplazes, D.P. Fedorko, C. Franzen, U. Futh, F. Hunger, A. Lehmacher, C.G. Meyer, J.M. Molina, J. Sandfort, R. Weber, and T. Loscher Blinded, externally controlled multicenter evaluation of light microscopy and PCR for detection of microsporidia in stool specimens J. Clin. Microbiol. 36 1998 1814 1818
16. P. Schmid-Hempel, and R. Loosli A contribution to the knowledge of Nosema infections in bumble bees, Bombus spp Apidologie 29 1998 525 535
17. J.A. Shykoff, and P. Schmid-Hempel Incidence and effects of four parasites in natural populations of bumble bees in Switzerland Apidologie 22 1991 117 125
18. W.T. Tay, E.M. O'Mahony, and R.J. Paxton Complete rRNA gene sequence reveal that the microsporidium Nosema bombi infects diverse bumble bee (Bombus spp.) hosts yet contains multiple polymorphic sites J. Eucaryot. Microbiol. 52 2005 505 513
19. J.D. Thompson, D.G. Higgins, and T.J. Gibson Clustal-W-Improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice Nucleic Acids Res. 22 1994 4673 4680
20. S.M. Valles, D.H. Oi, O.P. Perera, and D.F. Williams Detection of Thelohania solenopsae (Microsporidia: Thelohaniidae) in Solenopsis invicta (Hymenoptera: Formicidae) by multiplex PCR J. Invertebr. Pathol. 81 2002 196 201
21. J. Van den Eijnde, and N. Vette Nosema infection in honey bees (Apis mellifera L.) and bumble bees (Bombus terrestris L.) Proc. Sect. Exp. Appl. Entomol. Neth. Entomol. Soc. 4 1993 205 208
22. J. Van den Eijnde, A. de Ruijter, and J. Van der Steen Method for rearing Bombus terrestris continuously and the production of bumble bee colonies for pollination purposes Acta Hortic. 288 1991 154 158
23. van Doorn, A., Velthuis, H., 2004. Developments in breeding, commercialization and economic value of bumblebees. In: Bernardinelli, I., Milani, N. (Ed.), Proceedings of the First European Conference of Apidology, Udine, Italy, September 2004. p. 71.
24. C. Van Heemert, A. De Ruijter, J. Van den Eijnde, and J. Van der Steen Year-round production of bumble bee colonies for crop pollination Bee World 71 1990 54 56
25. P.S. Walsh, D.A. Metzger, and R. Higuschi Chelex 100 as a medium for simple extraction of DNA for PCR-based typing from forensic material Biotechniques 10 1991 506 513
26. R. Weber, D.A. Schwartz, and P. Deplazes Laboratory diagnosis of microsporidiosis M. Wittner L.M. Weiss The Microsporidia and Microsporidiosis 1999 American Society for Microbiology Washington, DC 315 362
27. T.C. Webster, K.W. Pomper, G. Hunt, E.M. Thacker, and S.C. Jones Nosema apis infection in worker and queen Apis mellifera Apidologie 35 2004 49 54
28. L.M. Weiss, and C.R. Vossbrinck Molecular biology, molecular phylogeny, and molecular diagnostic approaches to the microsporidia M. Wittner L.M. Weiss The Microsporidia and Microsporidiosis 1999 American Society for Microbiology Washington, DC 129 171
29. R. Whittington, and M.L. Winston Effects of Nosema bombi and its treatment fumagillin on bumble bee (Bombus occidentalis) colonies J. Invertebr. Pathol. 84 2003 54 58