Expression of a soluble functional form of the integrin α4β1 in mammalian cells

Cell Adhesion and Communication

Volumn 7, Issue 5, 2000, Pages 377-390

  Stephens, P E ^a   Ortlepp, S ^a   Perkins, V C ^a   Robinson, M K ^a   Kirby, H ^a  

^a UCB PHARMA   (Belgium)

Author keywords

Fc fusion protein; Immunoadhesin; Soluble integrins; VCAM 1; VLA 4; 4 1

Indexed keywords

COMPLEMENTARY DNA; DIMER; HYBRID PROTEIN; IMMUNOGLOBULIN FC FRAGMENT; INTEGRIN; MUTANT PROTEIN; PROTEIN SUBUNIT; VASCULAR CELL ADHESION MOLECULE 1;

ANIMAL CELL; ARTICLE; CELL LINE; CONTROLLED STUDY; DISSOCIATION CONSTANT; HUMAN; HUMAN CELL; NONHUMAN; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN DOMAIN; PROTEIN EXPRESSION;

ANIMALIA; MAMMALIA;

EID: 31844457147     PISSN: 10615385     EISSN: None     Source Type: Journal    
DOI: 10.3109/15419060009109020     Document Type: Article

References (43)

1. Argraves, W.S., Suzuki, S., Arai, H., Thompson, K., Pierschbacher, M.D. and Ruoslahti, E. (1987) Amino acid sequence of the human fibronectin receptor. Journal of Cell Biology, 105, 1183-1190.
2. Baneres, J.L., Roquet, F., Green, M., LeCalvez, H. and Parello, J. (1998) The cation-binding domain from the alpha subunit of integrin alpha5 beta1 is a minimal domain for fibronectin recognition. Journal of Biological Chemistry, 273, 24744-24753.
3. Bebbington, C. (1995) Use of vectors based on gene amplification for the expression of cloned genes in mammalian cells. In Glover, D.M. and Hames, B.D. (Eds.) DNA Cloning 4, IRL Press, pp. 85-110.
4. Bebbington, C.R., Renner, G., Thomson, S., King, D., Abrams, D. and Yarranton, G.T. (1992) High-level expression of a recombinant antibody from myeloma cells using a glutamine synthetase gene as an amplifiable selectable marker. Bio/Technology, 10, 169-175.
5. Bednarczyk, J.L., Szabo, M.C. and McIntyre, B.W. (1992) Post-translational processing of the leukocyte integrin alpha 4 beta 1. Journal of Biological Chemistry, 267, 25274-25281.
6. Blue, M.L., Davis, G., Conrad, P. and Kelley, K. (1993) Specific cleavage of the alpha 4 integrin associated with activation of peripheral T lymphocytes. Immunology, 78, 80-85.
7. Briesewitz, R., Kern, A. and Marcantonio, E.E. (1993) Ligand-dependent and -independent integrin focal contact localization: the role of the alpha chain cytoplasmic domain. Molecular Biology of the Cell, 4, 593-604.
8. Cierniewski, C.S., Byzova, T., Papierak, M., Haas, T.A., Niewiarowska, J., Zhang, L., Cieslak, M. and Plow, E.F. (1999) Peptide ligands can bind to distinct sites in integrin alpha11b/beta3 and elicit different functional responses. Journal of Biological Chemistry, 274, 16923-16932.
9. Cockett, M.I., Bebbington, C.R. and Yarranton, G.T. (1991) The use of engineered E1A genes to transactivate the hCMV-MIE promoter in permanent CHO cell lines. Nucleic Acids Research, 19, 319-325.
10. Cox, D., Aoki, T., Seki, J., Motoyama, Y. and Yoshida, K. (1994) The pharmacology of the integrins. [Review] [356 refs]. Medicinal Research Reviews, 14, 195-228.
11. Denda, S., Muller, U., Crossin, K.L., Erickson, H.P. and Reichardt, L.F. (1998) Utilization of a soluble integrin-alkaline phosphatase chimera to characterize integrin alpha 8 beta 1 receptor interactions with tenascin: murine alpha 8 beta 1 binds to the RGD site in tenascin-C fragments, but not to native tenascin-C. Biochemistry, 37, 5464-5474.
12. De Melker, A.A. and Sonnenberg, A. (1996) The role of the cytoplasmic domain of alpha 6 integrin in the assembly and function of alpha 6 beta 1 and alpha 6 beta 4. European Journal of Biochemistry, 241, 254-264.
13. Eble, J.A., Wucherpfennig, K.W., Gauthier, L., Dersch, P., Krukonis, E., Isberg, R.R. and Hemler, M.E. (1998) Recombinant soluble human alpha 3 beta 1 integrin: purification, processing, regulation, and specific binding to laminin-5 and invasin in a mutually exclusive manner. Biochemistry, 37, 10945-10955.
14. Gazdar, A.F., Carney, D.N., Bunn, P.A., Russell, E.K., Jaffe, E.S., Schechter, G.P. and Guccion, J.G. (1980) Mitogen requirements for the in vitro propagation of cutaneous T-cell lymphomas. Blood, 55, 409-417.
15. Gulino, D., Boudignon, C., Zhang, L.Y., Concord, E., Rabiet, M.J. and Marguerie, G. (1992) Ca(2+)-binding properties of the platelet glycoprotein IIb ligand-interacting domain. Journal of Biological Chemistry, 267, 1001-1007.
16. Gulino, D., Martinez, P., Delachanal, E., Concord, E., Duperray, A., Alemany, M. and Marguerie, G. (1995) Expression and purification of a soluble functional form of the platelet alpha IIb beta 3 integrin. European Journal of Biochemistry, 227, 108-115.
17. Huang, C., Lu, C. and Springer, T.A. (1997) Folding of the conserved domain but not of flanking regions in the integrin beta2 subunit requires association with the alpha subunit. Proceedings of the National Academy of Sciences of the United States of America, 94, 3156-3161.
18. Kabat, E.A., Wu, T.T., Reid-Miller, M., Perry, H.M. and Gottesman, K.S. (1987) Sequences of Proteins of Immunological Interest. United States Department of Health and Human Services. Washington D.C.
19. Kassner, P.D., Kawaguchi, S. and Hemler, M.E. (1994) Minimum alpha chain cytoplasmic tail sequence needed to support integrin-mediated adhesion. Journal of Biological Chemistry. 269, 19859-19867.
20. Laemmli, U.K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, 227, 680.
21. LaFlamme, S.E., Akiyama, S.K. and Yamada, K.M. (1992) Regulation of fibronectin receptor distribution [published erratum in J. Cell Biol., 1992 118(2), 491]. Journal of Cell Biology, 117, 437-447.
22. Lobb, R.R., Antognetti, G., Pepinsky, R.B., Burkly, L.C., Leone, D.R. and Whitty, A. (1995) A direct binding assay for the vascular cell adhesion molecule-1 (VCAM1) interaction with alpha 4 integrins. Cell Adhesion & Communication, 3, 385-397.
23. Lobb, R.R., Abraham, W.M., Burkly, L.C., Gill, A., Ma, W., Knight, J.A., Leone, D.R., Antognetti, G. and Pepinsky, R.B. (1996) Pathophysiologic role of alpha 4 integrins in the lung. [Review] [47 refs]. Annals of the New York Academy of Sciences, 796, 113-123.
24. Lu, C.F. and Springer, T.A. (1997) The alpha subunit cytoplasmic domain regulates the assembly and adhesiveness of integrin lymphocyte function-associated antigen-1. Journal of Immunology, 159, 268-278.
25. McKay, B.S., Annis, D.S., Honda, S., Christie, D. and Kunicki, T.J. (1996) Molecular requirements for assembly and function of a minimized human integrin alphaIIbbeta3. Journal of Biological Chemistry, 271, 30544-30547.
26. Mehta, R.J., Diefenbach, B., Brown, A., Cullen, E., Jonczyk, A., Gussow, D., Luckenbach, G.A. and Goodman, S.L. (1998) Transmembrane-truncated alphavbeta3 integrin retains high affinity for ligand binding: evidence for an 'inside-out' suppressor? Biochemical Journal, 330, 861-869.
27. Metzger, W.J. (1995) Therapeutic approaches to asthma based on VLA-4 integrin and its counter receptors. [Review] [65 refs]. Springer Seminars in Immunopathology, 16, 467-478.
28. 2+. Journal of Biological Chemistry, 270, 26270-26277.
29. Ortlepp, S., Stephens, P.E., Hogg, N., Figdor, C.G. and Robinson, M.K. (1995) Antibodies that activate beta 2 integrins can generate different ligand binding states. European Journal of Immunology, 25, 637-643.
30. Osborn, L., Hession, C., Tizard, R., Vassallo, C., Luhowskyj, S., Chi-Rosso, G. and Lobb, R. (1989) Direct expression cloning of vascular cell adhesion molecule 1, a cytokine-induced endothelial protein that bind to lymphocytes. Cell, 59, 1203-1211.
31. Pardi, R., Bossi, G., Inverardi, L., Rovida, E. and Bender, J.R. (1995) Conserved regions in the cytoplasmic domains of the leukocyte integrin alpha L beta 2 are involved in endoplasmic reticulum retention, dimerization, and cytoskeletal association. Journal of Immunology, 155, 1252-1263.
32. Peterson, J.A., Visentin, G.P., Newman, P.J. and Aster, R.H. (1998) A recombinant soluble form of the integrin alpha IIb beta 3 (GPIIb-IIIa) assumes an active, ligand-binding conformation and is recognized by GPIIb-IIIa-specific monoclonal, allo-, auto-, and drug-dependent platelet antibodies. Blood, 92, 2053-2063.
33. Ridgway, J.B., Presta, L.G. and Carter, P. (1996) 'Knobs-into-holes' engineering of antibody CH3 domains for heavy chain heterodimerization. Protein Engineering, 9, 617-621.
34. Sonnenberg, A. (1993) Integrins and their ligands. [Review] [241 refs]. Current Topics in Microbiology & Immunology, 184, 7-35.
35. Stephens, P.E. and Cockett, M.I. (1989) The construction of a highly efficient and versatile set of mammalian expression vectors. Nucleic Acids Research, 17, 7110.
36. Szabo, M.C. and McIntyre, B.W. (1995) Identification of two variants of the human Integrin alpha-4 subunit. Molecular Immunology, 32, 1453-1454.
37. Takada, O., Elices, M., Grouse, C. and Hemler, M. (1989) The primary structure of the a4 subunit of VLA-4: homology of other integrins and a possible cell-cell adhesion function. EMBO Journal, 8, 1361-1368.
38. Takahashi, N., Ueda, S., Obata, M., Nikaido, T., Nakai, S. and Honjo, T. (1982) Structure of human immunoglobulin gamma genes: implications for evolution of a gene family. Cell, 29, 671-679.
39. Teixido, J., Parker, C.M., Kassner, P.D. and Hemler, M.E. (1992) Functional and structural analysis of VLA-4 integrin alpha 4 subunit cleavage. Journal of Biological Chemistry, 267, 1786-1791.
40. Tominaga, Y., Kita, Y., Uchiyama, T., Sato, K., Takashi, T. and Horiuchi, T. (1998) Expression of a soluble form of LFA-1 and demonstration of its binding activity with ICAM-1. Journal of Immunological Methods, 212, 61-68.
41. Tomonaga, M., Gasson, J.C., Quan, S.G. and Golde, D.W. (1986) Establishment of eosinophillic sublines from human promyelocytic leukemia (HL60) cells: demonstration of mulitpotentiality and single-lineage commitment of HL-60 stem cells. Blood, 67, 1433-1441.
42. Whittle, N., Adair, J., Lloyd, C., Jenkins, E., Devine, J., Schlom, J., Raubitschek, A., Colcher, D. and Bodmer, M. (1987) Expression in COS cells of a mouse-human chimaeric B72.3 antibody. Protein Engineering, 1, 499-505.
43. Yauch, R.L., Felsenfeld, D.P., Kraeft, S.K., Chen, L.B., Sheetz, M.P. and Hemler, M.E. (1997) Mutational evidence for control of cell adhesion through integrin diffusion/ clustering, independent of ligand binding. Journal of Experimental Medicine, 186, 1347-1355.