Analysis of essential carboxylic amino acid residues for catalytic activity of fungal chitosanases by site-directed mutagenesis

Journal of Bioscience and Bioengineering

Volumn 100, Issue 5, 2005, Pages 545-550

  Shimosaka, Makoto ^a   Sato, Kazuaki ^a   Nishiwaki, Naohide ^a   Miyazawa, Takashi ^a   Okazaki, Mitsuo ^a  

^a SHINSHU UNIVERSITY   (Japan)

Author keywords

Chitosanase; Family 75 of glycosyl hydrolases; Fusarium solani; Inverting enzyme; Site directed mutagenesis

Indexed keywords

CATALYSIS; CATALYST ACTIVITY; ENZYMES; FUNGI; HYDROLYSIS; NUCLEAR MAGNETIC RESONANCE;

KINETIC ANALYSIS; MUTATED GENES; REACTION PRODUCTS;

AMINO ACIDS;

AMINO ACID; CARBOXYLIC ACID; CHITOSANASE; GLUCOSAMINE; GLYCOSIDASE;

AMINO ACID ANALYSIS; ARTICLE; CATALYSIS; CIRCULAR DICHROISM; ESCHERICHIA COLI; FUNGUS; FUSARIUM SOLANI; GENE MUTATION; HYDROLYSIS; KINETICS; NONHUMAN; PROTON NUCLEAR MAGNETIC RESONANCE; RELATIVE DENSITY; SACCHAROMYCES CEREVISIAE; SITE DIRECTED MUTAGENESIS; SPECTROSCOPY; WILD TYPE;

AMINO ACID SUBSTITUTION; FUNGAL PROTEINS; FUSARIUM; GLYCOSIDE HYDROLASES; POINT MUTATION; PROTEIN STRUCTURE, SECONDARY; SACCHAROMYCES CEREVISIAE; SUBSTRATE SPECIFICITY;

ESCHERICHIA COLI; FUNGI; FUSARIUM SOLANI; SACCHAROMYCES CEREVISIAE;

EID: 31844452565     PISSN: 13891723     EISSN: None     Source Type: Journal    
DOI: 10.1263/jbb.100.545     Document Type: Article

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