Simultaneous cell lysis and bead trapping in a continuous flow microfluidic device

Sensors and Actuators, B: Chemical

Volumn 113, Issue 2, 2006, Pages 944-955

  Ramadan, Qasem ^a,b   Samper, Victor ^a,d,e   Poenar, Daniel ^a,b   Liang, Zhu ^c   Yu, Chen ^a,f   Lim, T M ^c,g  

^a INSTITUTE OF MICROELECTRONICS   (Singapore)

^b NANYANG TECHNOLOGICAL UNIVERSITY   (Singapore)

^c NATIONAL UNIVERSITY OF SINGAPORE   (Singapore)

^d INSTITUTE OF BIOENGINEERING AND NANOTECHNOLOGY   (Singapore)

^e INSTITUTE OF BIOENGINEERING   (Russian Federation)

^f MEMS Research Group   (China)

^g Multidisciplinary Biosensor Focused Interest Group

Author keywords

Cell lysis; Continuous flow; Dielectrophoresis; DNA extraction

Indexed keywords

CELL LYSIS; CONTINUOUS FLOW; DIELECTROPHORESIS (DEP); DNA EXTRACTION;

BIOASSAY; BLOOD; CHIP SCALE PACKAGES; DNA; ELECTROPHORESIS; SILICA;

FLUIDICS;

EID: 31844432658     PISSN: 09254005     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.snb.2005.04.018     Document Type: Article

References (19)

1. K.D. Cole, M.C. Tellez, and R.W. Blaksley Separation of different physical forms of plasmid DNA using a combination of low electric field strength and flow in porous media: effect of different field gradient and porosity of the media Electrophoresis 21 2000 1010 1017
2. D. Figeys, and D. Pinto Protomics on a chip: promising developments Electrophoresis 22 2001 208 216
3. C. Heller Principle of DNA separation with capillary electrophoresis Electrophoresis 22 2001 629 643
4. U. Zimmermann, P. Scheurich, G. Pilwat, and R. Benz Cells with manipulated functions: new perspectives for cell biology, medicine and technology Angew. Chem. Ed. Eng. 20 1981 325 345
5. T. Kotnik, G. Pucihar, M. Rebersek, D. Miklavcic, and L.M. Mir Role of pulse shape in cell membrane electropermeabilization Biochim. Biophys. Acta 1614 2003 193 200
6. S.W. Lee, H. Yowanto, and Y.C. Tai A micro cell lysis device Sens. Actuators A, Phys. 73 1999 74 79
7. Y.C. Lin, M. Li, C.S. Fan, and L.W. Wu A microchip for electroporation of primary endothelial cells Sens. Actuators A, Phys. 108 2003 12 19
8. T.Y. Tsong Electroporation of cell membranes Biophys. J. 60 1991 297 306
9. U. Zimmermann Electrical breakdown, electropermeabilization and electrofusion Rev. Physiol. Biochem. Pharmacol. 105 1986 175 256
10. M. Puc, S. Corovic, K. Flisar, M. Petkovsek, J. Nastran, and D. Miklavcic Techniques of signal generation required for electropermeabilization. Survey of electropermeabilization devices Bioelectrochemistry 64 2004 113 124
11. T. Ohshima, M. Sato, and M. Saito Selective release of intracellular protein using pulsed electric field J. Electrostatics 35 1995 103 112
12. H.A. Pohl Dielectrophoresis: The Behavior of Neutral Matter in Non-uniform Electric Fields 1978 Cambridge University Press
13. R. Pethig Dielectrophoresis: using inhomogeneous AC electrical fields to separate and manipulate cells Crit. Rev. Biotechnol. 16 1996 331 348
14. http://www.bangslabs.com/technotes/101
15. M.L. Smit, B.A. Giesendorfã, S.G. Heil, J.A. Vet, F.J.M. Trijbels, and H.J. Blom Automated extraction and amplification of DNA from whole blood using a robotic workstation and an integrated thermocycler Biotechnol. Appl. Biochem. 32 2000 121 125
16. V. Sukhorkov, G. Meedt, M. Kurschner, and U. Zimmermann A single-shell model for biological cells extended to account for the dielectric anisotropy of the plasma membrane J. Electrostatics 50 2001 191 204
17. A. Ramos, H. Morgan, N. Green, and A. Castellanos AC electrokinetics: a review of forces in microelectrodes structures J. Phys. D: Appl. Phys. 31 1998 2338 2353
18. http://www.matweb.com
19. M. Washizu, S. Suzuki, O. Kurosawa, T. Nishizaka, and T. Shinohara Molecular dielectrophoresis of biopolymers IEEE Trans. Ind. Appl. ED-30 1994 835 843