Microarrays of lentiviruses for gene function screens in immortalized and primary cells

Nature Methods

Volumn 3, Issue 2, 2006, Pages 117-122

  Bailey, Steve N ^a,b   Ali, Siraj M ^a,b   Carpenter, Anne E ^a,b   Higgins, Caitlin O ^a,b   Sabatini, David M ^a,b  

^a WHITEHEAD INSTITUTE FOR BIOMEDICAL RESEARCH   (United States)

^b BROAD INSTITUTE OF MIT AND HARVARD   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

COMPLEMENTARY DNA; GLASS; SHORT HAIRPIN RNA; VIRUS GLYCOPROTEIN;

ANIMAL CELL; ARTICLE; CELL CULTURE; CELL GROWTH; CELL IMMORTALIZATION; CELL TYPE; CONTROLLED STUDY; GENE CLUSTER; GENE FUNCTION; GENETIC CODE; GENETIC SCREENING; GENETIC TRANSDUCTION; HIGH THROUGHPUT SCREENING; HUMAN; HUMAN CELL; LENTIVIRINAE; MAMMAL CELL; MICROARRAY ANALYSIS; MONOLAYER CULTURE; MOUSE; NONHUMAN; PRIORITY JOURNAL; RETROVIRUS INFECTION; VESICULAR STOMATITIS VIRUS; VIRAL GENE DELIVERY SYSTEM;

ANIMALS; ANTIGENS, THY-1; CELL LINE, TRANSFORMED; CELL LINE, TUMOR; CELLS, CULTURED; DENDRITIC CELLS; FIBROBLASTS; GENE EXPRESSION REGULATION; GREEN FLUORESCENT PROTEINS; HELA CELLS; HUMANS; IMAGE PROCESSING, COMPUTER-ASSISTED; LAMIN TYPE A; LENTIVIRUS; LUMINESCENT PROTEINS; MEMBRANE GLYCOPROTEINS; MICE; MICROARRAY ANALYSIS; MICROSCOPY, FLUORESCENCE; PHOSPHORYLATION; PROMOTER REGIONS (GENETICS); PROTEIN KINASES; RECOMBINANT FUSION PROTEINS; RIBOSOMAL PROTEIN S6 KINASES; RNA INTERFERENCE; RNA, SMALL INTERFERING; TRANSFECTION; VIRAL ENVELOPE PROTEINS;

ANIMALIA; LENTIVIRUS; MAMMALIA; VESICULAR STOMATITIS VIRUS;

EID: 31744445957     PISSN: 15487091     EISSN: 15491676     Source Type: Journal    
DOI: 10.1038/nmeth848     Document Type: Article

References (14)

1. Brummelkamp, T.R. & Bernards, R. New tools for functional mammalian cancer genetics. Nat. Rev. Cancer 3, 781-789 (2003).
2. Ziauddin, J. & Sabatini, D.M. Microarrays of cells expressing defined cDNAs. Nature 411, 107-110 (2001).
3. Mousses, S. et al. RNAi microarray analysis in cultured mammalian cells. Genome Res. 13, 2341-2347 (2003).
4. Silva, J.M., Mizuno, H., Brady, A., Lucito, R. & Hannon, G.J. RNA interference microarrays: High-throughput loss-of-function genetics in mammalian cells. Proc. Natl. Acad. Sci. USA 101, 6548-6552 (2004).
5. Yoshikawa, T. et al. Transfection microarray of human mesenchymal stem cells and on-chip siRNA gene knockdown. J. Control. Release 96, 227-232 (2004).
6. Vanhecke, D. & Janitz, M. High-throughput gene silencing using cell arrays. Oncogene 23, 8353-8358 (2004).
7. Federico, M. (ed.). Lentiviral Gene Engineering Protocols (Humana Press, Totowa, New Jersey, 2003).
8. Stewart, S.A. et al. Lentivirus-delivered stable gene silencing by RNAi in primary cells. RNA 9, 493-501 (2003).
9. Rubinson, D.A. et al. A lentivirus-based system to functionally silence genes in primary mammalian cells, stem cells and transgenic mice by RNA interference. Nat. Genet. 33, 401-406 (2003).
10. Guertin, D.A. & Sabatini, D.M. An expanding role for mTOR in cancer. Trends Mol. Med. 11, 353-361 (2005).
11. How, S.E. et al. Polyplexes and lipoplexes for mammalian gene delivery: from traditional to microarray screening. Comb. Chem. High Throughput Screen. 7, 423-430 (2004).
12. Sarbassov, D.D. et al. Rictor, a novel binding partner of mTOR, defines a rapamycin-insesitive and raptor-independent pathway that regulates the cytoskeleton. Curr. Biol. 14, 1296-1302 (2004).
13. Naldini, L. et al. In vivo gene delivery and stable transduction of nondividing cells by a lentiviral vector. Science 272, 263-267 (1996).
14. Inaba, K. et al. Generation of large numbers of dendritic cells from mouse bone marrow cultures supplemented with granulocyte/macrophage colony-stimulating factor. J. Exp. Med. 176, 1693-1702 (1992).