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Volumn 45, Issue 2, 2006, Pages 493-495

High-resolution EXAFS of the active site of human sulfite oxidase: Comparison with density functional theory and X-ray crystallographic results

Author keywords

[No Author keywords available]

Indexed keywords

SULFITE OXIDASE;

EID: 31544439387     PISSN: 00201669     EISSN: None     Source Type: Journal    
DOI: 10.1021/ic0512274     Document Type: Article
Times cited : (40)

References (25)
  • 8
    • 31544433074 scopus 로고    scopus 로고
    • note
    • 6 The oxidized sample was prepared at a final concentration of approximately 1.7 mM in a mixed buffer system consisting of 20 mM Tris, bis-Tris, and bis-Tris propane (pH 9.0) with no added chloride and was frozen in a 10 mm x 10 mm x 3 mm leucite cell. The reduced sample was generated from the oxidized sample by the addition of excess dithionite and trace methylviologen.
  • 11
    • 31544481659 scopus 로고    scopus 로고
    • note
    • We note that these are arbitrarily chosen examples and that there are many possible solutions.
  • 13
    • 31544470312 scopus 로고    scopus 로고
    • note
    • The enzyme used in the XRD study was initially oxidized, but the structure has been assigned to a reduced form because photoreduction of the crystallographic sample is likely as a result of a number of factors.
  • 14
    • 31544450969 scopus 로고    scopus 로고
    • note
    • X-ray absorption near-edge spectra are highly sensitive to the electronic structure. We observed no changes in the near-edge spectrum throughout the course of our experiment, indicating that no significant photoreduction occurred.
  • 18
    • 31544478485 scopus 로고    scopus 로고
    • note
    • -5 Hartree were used, but no vibrational analyses were performed.
  • 22
    • 31544479028 scopus 로고    scopus 로고
    • note
    • VI and calculations were performed with the appropriate overall charge of 1- for water-bound structures and 2- for hydroxide-bound structures.
  • 24
    • 0000273676 scopus 로고    scopus 로고
    • Hille, R. Chem. Rev. 1996, 96, 2757-2816
    • (1996) Chem. Rev. , vol.96 , pp. 2757-2816
    • Hille, R.1


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.