Molecular investigation of genetic elements contributing to metronidazole resistance in Bacteroides strains

Journal of Antimicrobial Chemotherapy

Volumn 57, Issue 2, 2006, Pages 212-220

  Sóki, József ^a   Gal, Micaela ^b   Brazier, Jon S ^b   Rotimi, Vincent O ^c   Urbán, Edit ^a   Nagy, Elisabeth ^a   Duerden, Brian I ^b  

^a UNIVERSITY OF SZEGED   (Hungary)

^b UNIVERSITY HOSPITAL OF WALES   (United Kingdom)

^c KUWAIT UNIVERSITY   (Kuwait)

Author keywords

cfiA; Insertion sequence (IS) elements; nim genes; Plasmid functions

Indexed keywords

METRONIDAZOLE; TETRACYCLINE;

ANTIBIOTIC RESISTANCE; ARTICLE; BACTERIAL GENE; BACTERIAL GENETICS; BACTERIAL STRAIN; BACTEROIDES; BACTEROIDES FRAGILIS; BACTEROIDES OVATUS; BACTEROIDES THETAIOTAOMICRON; BACTEROIDES UNIFORMIS; CHROMOSOME; CONTROLLED STUDY; GENE ACTIVATION; GENE INSERTION SEQUENCE; GENE LOCATION; GENE SEQUENCE; NONHUMAN; NUCLEOTIDE SEQUENCE; PLASMID; POLYMERASE CHAIN REACTION; REPLICON; SOUTHERN BLOTTING;

ANTI-BACTERIAL AGENTS; BACTERIAL PROTEINS; BACTEROIDES; BETA-LACTAMASES; BLOTTING, SOUTHERN; CHROMOSOME MAPPING; CHROMOSOMES, BACTERIAL; DNA TRANSPOSABLE ELEMENTS; DNA, BACTERIAL; DRUG RESISTANCE, BACTERIAL; ELECTROPHORESIS, POLYACRYLAMIDE GEL; GENES, BACTERIAL; METRONIDAZOLE; MOLECULAR SEQUENCE DATA; NUCLEIC ACID HYBRIDIZATION; PLASMIDS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION;

EID: 31544435513     PISSN: 03057453     EISSN: 14602091     Source Type: Journal    
DOI: 10.1093/jac/dki443     Document Type: Article

References (41)

1. Rasmussen BA, Bush K, Tally FP. Antimicrobial resistance in Bacteroides. Clin Inf Dis 1993; 16 Suppl. 4: S390-400
2. Smith CJ, Tribble GD, Bayley DP. Genetic elements of Bacteroides species: A moving story. Plasmid 1998; 40: 12-29.
3. Haggoud A, Reysset G, Azeddoug H et al. Nucleotide sequence analysis of two 5-nitroimidazole resistance determinants from Bacteroides strains and of a new insertion sequence upstream of the two genes. Antimicrob Agents Chemother 1994; 38: 1047-51.
4. Trinh S, Haggoud A, Reysset G et al: Plasmids pIP419 and pIP421 from Bacteroides: 5-nitroimidazole resistance genes and their upstream insertion sequence elements. Microbiology 1995; 141: 927-35.
5. Stubbs SL, Brazier JS, Talbot PR et al. PCR-restriction fragment length polymorphism analysis for identification of Bacteroides spp. and characterization of nitroimidazole resistance genes. J Clin Microbiol 2000; 38: 3209-13.
6. Gal M, Brazier JS. Metronidazole resistance in Bacteroides spp. carrying nim genes and the selection of slow-growing metronidazole-resistant mutants. J Antimicrob Chemother 2004; 54: 109-16.
7. Löfmark S, Fang H, Hedberg M et al. Inducible metronidazole resistance and nim genes in clinical Bacteroides fragilis group isolates. Antimicrob Agents Chemother 2005; 49: 1253-56.
8. Sebald M, Reysset G, Breuil J. What's new in 5-nitro-imidazole resistance in the Bacteroides fragilis group? In: Borrielo SP, ed. Clinical and molecular aspect of anaerobes. Petersfield: Wrightson Biomedical Publishing Ltd., 1990; 217-25.
9. Trinh S, Haggoud A, Reysset G. Conjugal transfer of the 5-nitroimidazole resistance plasmid pIP417 from Bacteroides vulgatus BV-17: characterization and nucleotide sequence analysis of the mobilization region. J Bacteriol 1996; 178: 6671-76.
10. Trinh S, Reysset G. Identification and DNA sequence of the mobilization region of the 5-nitroimidazole resistance plasmid pIP421 from Bacteroides fragilis. J Bacteriol 1997; 179: 4071-74.
11. Reysset G. Genetics of 5-nitroimidazole resistance in Bacteroides species. Anaerobe 1996; 2: 59-69.
12. Sóki J, Fodor E, Hecht DW et al. Molecular characterization of imipenem-resistant, cfiA-positive Bacteroides fragilis isolates from the USA, Hungary and Kuwait. J Med Microbiol 2004; 53: 413-9.
13. Podglajen I, Breuil J, Bordon F et al. A silent carbapenemase gene in strains of Bacteroides fragilis can be expressed after a one-step mutation. FEMS Microbiol Lett 1992; 91: 21-9.
14. Podglajen I, Breuil J, Casin I et al. Genotypic identification of two groups within the species Bacteroides fragilis by ribotyping and by analysis of PCR-generated fragment patterns and insertion sequence content. J Bacteriol 1995; 177: 5270-75.
15. Ruimy R, Podglajen I, Breuil J et al. A recent fixation of cfiA genes in a monophyletic cluster of Bacteroides fragilis is correlated with the presence of multiple insertion elements. J Bacteriol 1996; 178: 1914-18.
16. Gutacker M, Valsangiacomo C, Piffaretti J-C. Identification of two genetic groups in Bacteroides fragilis by multilocus enzyme electrophoresis: Distribution of antibiotic resistance (cfiA, cepA) and enterotoxin (bft) encoding genes. Microbiology 2000; 146: 1241-54.
17. Gutacker M, Valsangiacomo C, Bernasconi MV et al. RecA and glnA sequences separate the Bacteroides fragilis population into two genetic divisions associated with the antibiotic resistance genotypes cepA and cfiA. J Med Microbiol 2002; 51: 123-30.
18. Haggoud A, M'Hand RA, Reysset G et al. Prevalence and characteristics of nim genes encoding 5-nitroimidazole resistance among Bacteroides strains isolated in Morocco. Microb Drug Resist 2001; 7: 177-81.
19. Lerios H-K, Kozieiski-Stuhrmann S, Kapp U et al. Structural basis of 5-nitroimidazole antibiotic resistance: The crystal structure of NimA from Deinococcus radiodurans. J Biol Chem 2004; 279: 55840-49.
20. Jamal WY, Rotimi VO, Brazier JS et al. Molecular characterization of nitroimidazole resistance in metronidazole-resistant Bacteroides species isolated from hospital patients in Kuwait. Med Princ Pract 2004; 13: 147-52.
21. National Committee of Clinical Laboratory Standards. Methods for Antimicrobial Susceptibility Testing of Anaerobic Bacteria - Fifth Edition: Approved Standard M11-A6. NCCLS, Wayne, PA, USA, 2001.
22. Mays TD, Smith CJ, Welch RA et al. Novel antibiotic resistance transfer of Bacteroides. Antimicrob Agents Chemother 1982; 21: 110-8.
23. Urbán E, Sóki J, Brazier JS et al. Prevalence and characterization of nim genes of Bacteroides spp. isolated in Hungary. Anaerobe 2002; 8: 175-9.
24. Wilson K. Miniprep of bacterial genomic DNA. In: Ausubel FM, Brent R, Kingston RE et al., ed. Current protocols in molecular biology. NY: John Wiley and Sons, Inc. 1994; 2.4.1-4.2.
25. Sambrook J, Fritsch EF, Maniatis T, ed. Molecular Cloning. A Laboratory Manual. NY: Cold Spring Harbour Laboratory Press 1989.
26. Trinh S, Reysset G. Detection by PCR of the nim genes encoding 5-nitroimidazole resistance in Bacteroides spp. J Clin Microbiol 1996; 34: 2078-84.
27. Boybek A, Ray TD, Evans MC et al. Novel site-specific DNA modification in Streptomyces: Analysis of preferred intragenic modification sites present in a 5.7 kb amplified DNA sequence. Nucleic Acids Res 1998; 26: 3364-71.
28. Corkhill JE, Graham R, Hart CA et al. Pulsed-field gel electrophoresis of degradation sensitive DNAs from Clostridium difficile PCR ribotype 1 strains. J Clin Microbiol 2000; 38: 2791-92.
29. Diniz CG, Farias LM, Carvalho MA et al. Differential gene expression in a Bacteroides fragilis metronidazole-resistant mutant. J Antimicrob Chemother 2004; 54: 100-8.
30. Salyers AA, Shoemaker NB, Li L-Y. In the driver's seat: The Bacteroides conjugative transposons and the elements they mobilize. J Bacteriol 1995; 177: 5727-31.
31. Marchandin H, Jean-Pierre H, Campos J et al. nimE gene in a metronidazole-susceptible Veillonella sp. strain. Antimicrob Agents Chemother 2004; 48: 3207-8.
32. Toussaint A, Merlin C. Mobile elements as a combination of functional modules. Plasmid 2002; 47: 26-35.
33. Galgan JE, Nusbaum C, Roy A et al. The genome of M. acetivorans reveals extensive metabolic and physiological diversity. Genome Res 2002; 12: 532-42.
34. Hedberg M, Nord CE, ESCMID Study Group on Antimicrobial Resistance in Anaerobic Bacteria. Antimicrobial susceptibility of Bacteroides fragilis group isolates in Europe. Clin Microbiol Infect 2003; 9: 475-88.
35. Snydman DR, Jacobus NV, McDermott LA et al. National survey on the susceptibility of Bacteroides fragilis Group: Report and analysis of trends for 1997-2000. Clin Infect Dis 2002; 35 Suppl 1: S126-34.
36. Johnson JL, Ault DA. Taxonomy of Bacteroides. II. Correlation of phenotypic characteristics with DNA homology grouping for Bacteroides fragilis and other saccharolytic Bacteroides species. Int J Syst Bacteriol 1978; 28: 257-68.
37. Lubbe MM, Stanley K, Chalkley W. Prevalence of nim genes in anaerobic/ facultative anaerobic bacteria isolated in South Africa. FEMS Microbiol Lett 1999; 172: 79-83.
38. Theron MM, Janse van Rensburg MN, Chalkley LJ. Nitroimidazole resistance genes (nimB) in anaerobic Gram-positive cocci (previously Peptostreptococcus spp.) J Antimicrob Chemother 2004; 54: 240-2.
39. Podglajen I, Breuil J, Rohaut A et al. Multiple mobile promoter regions for the rare carbapenem resistance gene of Bacteroides fragilis. J Bacteriol 2004; 183: 3531-5.
40. Kato N, Yamazoe K, Han C-G. et al. New insertion sequence elements in the upstream region of cfiA in imipenem-resistant Bacteroides fragilis strains. Antimicrob Agents Chemother 2003; 47: 979-85.
41. Haggoud A, Trinh S, Moumni M et al. Genetic analysis of the minimal replicon of plasmid pIP417 and comparison with the other encoding 5-nitroimidazole resistance plasmids from Bacteroides spp. Plasmid 1995; 34: 132-43.