Hg(II) exposure exacerbates UV-induced DNA damage in MRC5 fibroblasts: A comet assay study

Journal of Environmental Science and Health - Part A Toxic/Hazardous Substances and Environmental Engineering

Volumn 41, Issue 2, 2006, Pages 143-148

  Bradfield, Wendy ^a   Pye, Andrew ^a   Clifford, Tim ^a   Salter, Leo ^a   Gould, David ^a   Campbell, Sandra ^a   Curnow, Alison ^a  

^a ROYAL CORNWALL HOSPITAL   (United Kingdom)

Author keywords

Comet assay; DNA damage; Mercury; UV

Indexed keywords

ASSAYS; CARCINOGENS; CHLORINE COMPOUNDS; MERCURY (METAL); ULTRAVIOLET RADIATION;

COMET ASSAY; DNA-DAMAGE; MERCURIC CHLORIDE; MRC5 FIBROBLASTS: A COMET ASSAY STUDY;

DNA;

MERCURIC CHLORIDE;

ARTICLE; CARCINOGENESIS; COMET ASSAY; CONTROLLED STUDY; DNA DAMAGE; ENVIRONMENT; ENVIRONMENTAL EXPOSURE; FIBROBLAST; HUMAN; HUMAN CELL; INCUBATION TIME; SENSITIVITY ANALYSIS; SUNLIGHT; ULTRAVIOLET IRRADIATION;

COMET ASSAY; DNA DAMAGE; DOSE-RESPONSE RELATIONSHIP, DRUG; FIBROBLASTS; HUMANS; LUNG NEOPLASMS; MERCURIC CHLORIDE; TUMOR CELLS, CULTURED; ULTRAVIOLET RAYS;

EID: 31544433139     PISSN: 10934529     EISSN: 15324117     Source Type: Journal    
DOI: 10.1080/10934520500349243     Document Type: Article

References (19)

1. Ozuah, P. Mercury poisoning. Curr. Probl. Pediatr. 2000, 30, 91-99.
2. Clarkson, T.W. The three modern faces of mercury. Environ. Health Perspect. 2002, 110(Supplement 1), 11-23.
3. Magos, L.; Brown, A.W.; Sparrow, S.; Bailey, E.; Snowden, R.E.; Skipp, W.R. The comparative toxicology of ethyl- and methyl mercury. Arch. Toxicol. 1985, 57, 260-267.
4. Stacey, N.H.; Kappus, H. Cellular toxicity and lipid peroxidation in response to mercury. Toxicol. Appl. Pharmacol. 1982, 63, 29-35.
5. 2 formation and oxidative stress in vivo and in vitro in rat kidney mitochondria. Biochem. Pharmacol. 1993, 45, 2017-2024.
6. Hussain, S.; Rodgers, D.A.; Duhart, H.M.; Ali, S.F. Mercuric chloride-induced reactive oxygen species and its effect on antioxidant enzymes in different regions of rat brain. J. Environ. Sci. Health B 1997, 32, 395-409.
7. Hughes, W.L. A physicochemical rationale for the biological activity of mercury and its compounds. Ann. N. Y. Acad. Sci. 1957, 65, 454-460.
8. Simpson, R.B. Association constants of methylmercury with sulfhydryl and other bases. J. Am. Chem. Soc. 1961, 83, 4711-4717.
9. Cookson, M.R.; Pentreath, V.W. Protective Roles of Glutathione in the Toxicity of Mercury and Cadmium Compounds to C6 Glioma Cells. Toxicol. In Vitro. 1996, 10, 257-264.
10. Ballatori, N.; Clarkson, T.W. Dependence of biliary secretion of inorganic mercury on the kidney transport of glutathione. Biochem. Pharmacol. 1984, 33, 1093-1098.
11. Lee, Y.W.; Ha, M.S.; Kim, Y.K. Role of reactive oxygen species and glutathione in inorganic mercury-induced injury in human glioma cells. Neurochem. Res. 2001, 26, 1187-1193.
12. Woods, J.S.; Calas, C.A.; Aicher, L.D.; Robinson, B.H.; Mailer, C. Stimulation of porphyrinogen oxidation by mercuric ion. I. Evidence of free radical formation in the presence of thiols and hydrogen peroxide. Mol. Pharmacol. 1990, 38, 253-260.
13. Sanders, B.M.; Goering, P.L.; Jenkins, K. The role of general and metal-specific cellular responses in protection and repair of metal-induced damage: Stress proteins and metallothioneins. In: Toxicology of Metals 1996. Ed.: Chang, L.W. CRC Press, Boca Raton, Florida, USA, pp. 165-187.
14. Shenker, B.J.; Guo, T.L.; Shapiro, I.M. Mercury-induced apoptosis in human lymphoid cells: evidence that apoptotic pathway is mercurial species dependent. Environ. Res. 2000, 84(2), 89-99.
15. Singh, N.; McCoy, M.; Tice, R.; Scneider, E. A simple technique for quantification of low levels of DNA damage in individual cells. Exp. Cell Res. 1988, 175, 184-191.
16. Ramalingam, V.; Vimaladevi, V. Effect of mercuric chloride on membrane-bound enzymes in rat testis. Asian J. Androl. 2002, 4, 309-311.
17. Morley, N.; Curnow, A.; Salter, L.; Campbell, S.; Gould, D. N-acetyl-L-cysteine prevents DNA damage induced by UVA, UVB and visible radiation in human fibroblasts. J. Photochem. Photobiol. B. 2003, 72, 55-60.
18. Ben-Ozer, E.; Rosenspire, A.; McCabe, M.; Worth, R.; Kindzelskii, A.; Warra, N.; Petty, H. Mercuric chloride damages cellular DNA by a non-apoptotic mechanism. J. Mut. Res. 2000, 470, 19-27.
19. Kosan, C.; Topaloglu, A.; Ozkan, B. Chronic mercury intoxication simulating pheochromocytoma: effect of captopril on urinary mercury excretion. Pediatr. Int. 2001, 43, 429-430.