Dynamic profiling of the glucose metabolic network in fasted rat hepatocytes using [1,2-13C2]glucose

Biochemical Journal

Volumn 381, Issue 1, 2004, Pages 287-294

  Marin, Silvia ^a   Lee, W N Paul ^b   Bassilian, Sara ^b   Lim, Shu ^b   Boros, Laszlo G ^b   Centelles, Josep J ^a   Fernández Novell, Josep María ^a   Guinovart, Joan J ^a   Cascante, Marta ^a  

^a UNIVERSITY OF BARCELONA   (Spain)

^b UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

Futile cycles; GC MS; Glucose metabolism; Glucose stable isotope tracer; Metabolic network

Indexed keywords

ENZYMES; GLUCOSE; ISOMERS; METABOLISM; PHYSIOLOGY;

DYNAMIC PROFILING; INCUBATION;

BIOCHEMISTRY;

CARBON 13; GLUCOKINASE; GLUCOSE; GLUCOSE 6 PHOSPHATASE; TRACER;

ANIMAL CELL; ARTICLE; CELL ISOLATION; ENZYME ACTIVITY; GENOTYPE PHENOTYPE CORRELATION; GLUCONEOGENESIS; GLUCOSE METABOLISM; ISOTOPE LABELING; KINETICS; LIVER CELL; MALE; NONHUMAN; PHENOTYPE; PRIORITY JOURNAL; RAT;

ANIMALS; CARBON ISOTOPES; CITRIC ACID CYCLE; FASTING; GAS CHROMATOGRAPHY-MASS SPECTROMETRY; GENOTYPE; GLUCOKINASE; GLUCONEOGENESIS; GLUCOSE; GLUCOSE-6-PHOSPHATASE; GLYCOGEN; GLYCOLYSIS; HEPATOCYTES; LACTIC ACID; MALE; PHENOTYPE; RATS; RATS, WISTAR; SUBSTRATE CYCLING;

ANIMALIA; RATTUS NORVEGICUS;

EID: 3142755488     PISSN: 02646021     EISSN: None     Source Type: Journal    
DOI: 10.1042/BJ20031737     Document Type: Article

References (32)

1. Frazier, M. E., Johnson, G. M., Thomassen, D. G., Oliver, C. E. and Patrinos A. (2003) Realizing the potential of the genome revolution: the genomes to life program. Science (Washington, D.C.) 300, 290-293
2. Oltvai, Z. N. and Barabási, A.-L. (2002) Systems biology. Life's complexity pyramid. Science (Washington, D.C.) 298, 763-764
3. Hue, L. (1981) The role of futile cycles in the regulation of carbohydrate metabolism in the liver. Adv. Enzymol. Relat. Areas Mol. Biol. 52, 247-331
4. Katz, J. and Rognstad, R. (1976) Futile cycles in the metabolism of glucose. Curr. Top. Cell Regul. 10, 237-289
5. McGarry, J. D., Kuwajima, M., Newgard, C. B., Foster, D. W. and Katz, J. (1987) From dietary glucose to liver glycogen: the full circle round. Annu. Rev. Nutr. 7, 51-73
6. Price, N. D., Papin, J. A., Schilling, C. H. and Palsson, B. O. (2003) Genome-scale microbial in silico models: the constraints-based approach. Trends Biotechnol. 21, 162-169
7. Reed, J. L. and Palsson, B. O. (2003) Thirteen years of building constraint-based in silico models of Escherichia coli. J. Bacteriol. 185, 2692-2699
8. Boros, L. G., Steinkamp, M. P., Fleming, J. C., Lee, W. N., Cascante, M. and Neufeld, E. J. (2003) Defective RNA ribose synthesis in fibroblasts from patients with thiamine-responsive megaloblastic anemia (TRMA). Blood 102, 3556-3561
9. Comin-Anduix, B., Boros, L. G., Marin, S., Boren, J., Callol-Massot, C., Centelles, J. J., Torres, J. L., Agell, N., Bassilian, S. and Cascante, M. (2002) Fermented wheat germ extract inhibits glycolysis/pentose cycle enzymes and induces apoptosis through poly(ADP-ribose) polymerase activation in Jurkat T-cell leukemia tumor cells. J. Biol. Chem. 277, 46408-46414
10. Boros, L. G., Cascante, M. and Lee, W. N. (2002) Metabolic profiling of cell growth and death in cancer: applications in drug discovery. Drug Discov. Today 7, 364-372
11. Boren, J., Cascante, M., Marin, S., Comin-Anduix, B., Centelles, J. J., Lim, S., Bassilian, S., Ahmed, S., Lee, W. N. and Boros, L. G. (2001) Gleevec (STI571) influences metabolic enzyme activities and glucose carbon flow toward nucleic acid and fatty acid synthesis in myeloid tumor cells. J. Biol. Chem. 276, 37747-37753
12. Fernández-Novell, J. M., Ariño, J. and Guinovart, J. J. (1994) Effects of glucose on the activation and translocation of glycogen synthase in diabetic rat hepatocytes. Eur. J. Biochem. 226, 665-671
13. Chan, T. M., and Exton, J. H. (1976) A rapid method for the determination of glycogen content and radioactivity in small quantities of tissue or isolated hepatocytes. Anal. Biochem. 71, 96-105
14. Fernández-Novell, J. M., Ariño, J., Vilaró, S., Bellido, D. and Guinovart, J. J. (1992) Role of glucose 6-phosphate in the translocation of glycogen synthase in rat hepatocytes. Biochem. J. 288, 497-501
15. Kunst, A., Draeger, B. and Ziegenhorn, J. (1984) d-Glucose; UV-methods with hexolinase and glucose 6-phosphate dehydrogenase. In Methods of Enzymatic Analysis (Bergmeyer, H. U., ed.), vol. VI, pp. 163-172, Verlag Chemie, Weinheim
16. Gutmann, I. and Wahlefeld, A. W. (1974) L-(+)-Lactate. In Methods of Enzymatic Analysis (Bergmeyer, H. U., ed), vol. 3, pp. 1464-1468, Academic Press, NY
17. Tserng, K. Y., Gilfillan, C. A. and Kalhan, S. C. (1984) Determination of carbon-13 labeled lactate in blood by gas chromatography/mass spectrometry. Anal. Chem. 56, 517-523
18. 13C]glucose in rats. J. Biol. Chem. 264, 12994-13004
19. 13C]glycerol carbon via the pentose cycle. Implications for gluconeogenesis measurement by mass isotoper distribution analysis. J. Biol. Chem. 275, 36787-36793
20. Lee, W. P., Edmond, J., Bassilian, S. and Morrow, J. (1996) Mass isotopomer study of glutamine oxidation and synthesis in primary culture of astrocytes. Develop. Neurosci. 18, 469-477
21. Lee, W.-N. P., Byerley, L. O., Bergner, E. A. and Edmond, J. (1991) Mass isotopomer analysis: theoretical and practical considerations. Biol. Mass Spectrom. 20, 451-458
22. Katz, J., Wals, P. A. and Rognstad, R. (1978) Glucose phosphorylation, glucose 6-phosphatase, and recycling in rat hepatocytes. J. Biol. Chem. 253, 4530-4536
23. Gomis, R. R., Favre, C., Garcia-Rocha, M., Fernández-Novell, J. M., Ferrer, J. C. and Guinovart, J. J. (2003) Glucose 6-phosphate produced by gluconeogenesis and by glucokinase is equally effective in activating hepatic glycogen synthase. J. Biol. Chem. 278, 9740-9746
24. Seoane, J., Gomez-Foix, A. M., O'Doherty, R. M., Gomez-Ara, C., Newgard, C. B. and Guinovart, J. J. (1996) Glucose 6-phosphate produced by glucokinase, but not hexokinase I, promotes the activation of hepatic glycogen synthase. J. Biol. Chem. 271, 23756-23760
25. 13C2]glucose. Am. J. Physiol. 274, E843-E851
26. Shiota, M., Galassetti, P., Monohan, M., Neal, D. W. and Cherrington, A. D. (1998) Small amounts of fructose markedly augment net hepatic glucose uptake in the conscious dog. Diabetes 47, 867-873
27. Salhanick, A. L., Chang, C. L. and Amatruda, J. M. (1989) Hormone and substrate regulation of glycogen accumulation in primary cultures of rat hepatocytes. Biochem. J. 261, 985-992
28. Van Schaftingen, E. and Vandercammen, A. (1989) Stimulation of glucose phosphorylation by fructose in isolated rat hepatocytes. Eur. J. Biochem. 179, 173-177
29. Newsholme, E. A. and Stanley, J. C. (1987) Substrate cycles: their role in control of metabolism with specific references to the liver. Diabetes Metab. Rev. 3, 295-305
30. Newsholme, E. A., Arch, J. R., Brooks, B. and Surholt, B. (1983) The role of substrate cycles in metabolic regulation. Biochem. Soc. Trans. 11, 52-56
31. Huang, S. (2002) Rational drug discovery: what can we learn from regulatory networks? Drug Discov. Today 7, S163-S169
32. Stalmans, W., Laloux, M. and Hers, H. G. (1974) The interaction of liver phosphorylase A with glucose and AMP. Eur. J. Biochem. 49, 415-427