Vascular endothelial growth factor overexpression increases vascularization by murine but not human endothelial cells in cultured skin substitutes grafted to athymic mice

Journal of Burn Care and Rehabilitation

Volumn 25, Issue 4, 2004, Pages 337-345

  Supp, Dorothy M ^a,b   Karpinski, Andrea C ^a   Boyce, Steven T ^a,b  

^a SHRINERS HOSPITALS FOR CHILDREN   (United States)

^b UNIVERSITY OF CINCINNATI COLLEGE OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BIOPOLYMER; MITOGENIC AGENT; VASCULOTROPIN;

ADULT; ANIMAL CELL; ANIMAL TISSUE; ARTICLE; ARTIFICIAL SKIN; AUTOGRAFT; BURN; CELL CULTURE; CELL DENSITY; CONTROLLED STUDY; CULTURED SKIN SUBSTITUTE; DERMAL MICROVASCULAR ENDOTHELIUM CELL; DNA MODIFICATION; ENDOTHELIUM CELL; FEMALE; FIBROBLAST; GENE OVEREXPRESSION; HUMAN; HUMAN CELL; KERATINOCYTE; MICROVASCULATURE; MOUSE; NONHUMAN; NORMAL HUMAN; NUDE MOUSE; SKIN BLOOD VESSEL; SPLIT THICKNESS SKIN GRAFT; VASCULARIZATION;

ANIMALS; BURNS; ENDOTHELIUM, VASCULAR; FIBROBLASTS; HUMANS; KERATINOCYTES; MICE; MICE, NUDE; MODELS, ANIMAL; SKIN TRANSPLANTATION; SKIN, ARTIFICIAL; TISSUE CULTURE TECHNIQUES; VASCULAR ENDOTHELIAL GROWTH FACTORS;

EID: 3142733518     PISSN: 02738481     EISSN: None     Source Type: Journal    
DOI: 10.1097/01.BCR.0000132168.02947.A1     Document Type: Article

References (27)

1. Berthod F, Damour O. In vitro reconstructed skin models for wound coverage in deep burns. Br J Dermatol 1997;136:809-16.
2. Falanga V. Chronic wounds: pathophysical and experimental considerations. J Invest Dermatol 1993;100:721-5.
3. Phillips TJ, Gilchrest BA. Cultured epidermal grafts in the treatment of leg ulcers. Adv Dermatol 1990;5:33-48.
4. Balasubramani M, Kumar TR, Babu M. Skin substitutes: a review. Burns 2001;27:534-44.
5. Bellow YM, Falabella AF. Use of skin substitutes in dermatology. Dermatol Clin 2001;19:555-61.
6. Boyce ST, Supp DM. Skin substitutes: theoretical and developmental considerations. In: McCauley RL, editor. Functional and aesthetic reconstruction of burn patients. New York: Marcel Dekker. In press.
7. Boyce ST, Goretsky MJ, Greenhalgh DG, Kagan RJ, Rieman MT, Warden GD. Comparative assessment of cultured skin substitutes and native skin autograft for treatment of fullthickness burns. Ann Surg 1995;222:743-52.
8. Boyce ST, Kagan RJ, Yakuboff KP, et al. Cultured skin substitutes reduce donor skin harvesting for closure of excised, full-thickness burns. Ann Surg 2002;235:269-79.
9. Boyce ST, Glatter R, Kitzmiller WJ. Treatment of chronic wounds with cultured cells and biopolymers. Wounds 1995;7:24-9.
10. Harriger MD, Warden GD, Greenhalgh DG, Kagan RJ, Boyce ST. Pigmentation and microanatomy of skin regenerated from composite grafts of cultured cells and biopolymers applied to full-thickness burn wounds. Transplantation 1995;59:702-7.
11. Boyce ST, Supp AP, Harriger MD, Greenhalgh DG, Warden GD. Topical nutrients promote engraftment and inhibit wound contraction of cultured skin substitutes in athymic mice. J Invest Dermatol 1995;104:345-9.
12. Boyce ST, Harriger MD, Supp AP, Warden GD, Holder IA. Effective management of microbial contamination in cultured skin substitutes after grafting to athymic mice. Wound Rep Reg 1997;5:191-7.
13. Boyce ST, Kagan RJ, Meyer NA, Yakuboff KP, Warden GD. The 1999 Clinical Research Award. Cultured skin substitutes combined with Integra to replace native skin autograft and allograft for closure of full-thickness burns. J Burn Care Rehabil 1999;20:453-61.
14. Conn G, Soderman DD, Schaeffer M-T, Wile M, Hatcher VB, Thomas KA. Purification of a glycoprotein vascular endothelial cell mitogen from a rat glioma-derived cell line. Proc Natl Acad Sci USA 1990;87:1323-7.
15. Detmar M, Yeo KT, Nagy JA, et al. Keratinocyte-derived vascular permeability factor (vascular endothelial growth factor) is a potent mitogen for dermal microvascular endothelial cells J Invest Dermatol 1995;105:44-50.
16. Supp DM, Supp AP, Bell SM, Boyce ST. Enhanced vascularization of cultured skin substitutes generically modified to overexpress vascular endothelial growth factor. J Invest Dermatol 2000;114:5-13.
17. Supp DM, Boyce ST. Overexpression of vascular endothelial growth factor accelerates early vascularizafion and improves healing of genetically modified cultured skin subsfitutes. J Bum Care Rehabil 2002;23:10-20.
18. Supp DM, Wilson-Landy K, Boyce ST. Human dermal microvascular endothelial cells form vascular analogs in cultured skin substitutes after grafting to athymic mice. FASEB J 2002;16:797-804.
19. Boyce ST, Ham RG. Cultivation, frozen storage, and clonal growth of normal human epidermal kerafinocytes in serumfree media. J Tissue Cult Meth 1985;9:83-93.
20. Boyce ST. Methods for serum-free culture of keratinocytes and transplantation of collagen-GAG based composite grafts. In Morgan JR, Yarmush M, editors. Methods in tissue engineering. Totowa, NJ: Humana Press, Inc.; 1998. p. 365-89.
21. Swope VB, Supp AP, Greenhalgh DG, Warden GD, Boyce ST. Expression of insulin-like growth factor-I by cultured skin substitutes does not replace the physiologic requirement for insulin in vitro. J Invest Dermatol 2001;116:650-7.
22. Boyce ST, Foreman TJ, English KB, et al. Skin wound closure in athymic mice with cultured human cells, biopolymers, and growth factors. Surgery 1991;110:866-76.
23. Osborn M, Isenberg S. Immunocytochemistry of frozen and of paraffin tissue sections. In Celis JE, editor. Cell biology: a laboratory handbook. San Diego, CA: Academic Press; 1998. p. 486-92.
24. L'Heureux N, Germain L, Labbe R, Auger FA. In vitro construction of a human blood vessel from cultured vascular cells: a morphologic study. J Vasc Surg 1993;17:499-509.
25. Black AF, Berthod F, L'Heureux N, Germain L, Auger FA. In vitro of a reconstruction human capillary-like network in a tissue-engineered skin equivalent. FASEB J 1998;12:1331-40.
26. Hudon V, Berthod F, Black AF, Damour O, Germain L, Auger F. A tissue-engineered endothelialized dermis to study the modulation of angiogenic and angiostatic molecules on capillary-like tube formafion in vitro. Br J Dermatol 2003;148:1094-104.
27. Young DM, Greulich KM, Weier HG. Species-specific in situ hybridization with fluorochrome-labeled DNA probes to study vascularization of human skin grafts on athymic mice. J Burn Care Rehab 1996;17:305-10.