Characterization of the pCS20 region of different Ehrlichia ruminantium isolates

Veterinary Microbiology

Volumn 101, Issue 4, 2004, Pages 279-291

  Van Heerden, H ^a   Steyn, H C ^a   Allsopp, M T E P ^a   Zweygarth, E ^a   Josemans, A I ^a   Allsopp, B A ^a  

^a ONDERSTEPOORT VETERINARY INSTITUTE   (South Africa)

Author keywords

Ehrlichia (Cowdria) ruminantium; Heartwater; pCS20

Indexed keywords

DNA; PRIMER DNA;

ARTICLE; BACTERIUM ISOLATE; CHIMERA; CLONE; CONTROLLED STUDY; EHRLICHIA RUMINANTIUM; GENE AMPLIFICATION; GENE LOCATION; GENE SEQUENCE; GENETIC ANALYSIS; GENOME; NONHUMAN; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; SEQUENCE ANALYSIS;

ANIMALS; ARACHNID VECTORS; BASE SEQUENCE; DNA PRIMERS; DNA PROBES; DNA, BACTERIAL; EHRLICHIA RUMINANTIUM; FEMALE; GENOME, BACTERIAL; HEARTWATER DISEASE; MALE; MOLECULAR SEQUENCE DATA; PHYLOGENY; POLYMERASE CHAIN REACTION; RUMINANTS; SENSITIVITY AND SPECIFICITY; SEQUENCE ALIGNMENT; SEQUENCE HOMOLOGY, NUCLEIC ACID; TICKS;

ACARI; ANIMALIA; EHRLICHIA; EHRLICHIA RUMINANTIUM;

EID: 3142681194     PISSN: 03781135     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.vetmic.2004.02.015     Document Type: Article

References (29)

1. Bell-Sakyi, L., Koney, E.B.M., Dogbey, O., Abbam, J.A., Aning, K.G., 1997. Isolation and in vitro cultivation in Ghana of Cowdria ruminantium, the causative agent of heartwater. In: Koney, E.B.M., Aning, K.G. (Eds.), Proceedings of the 22nd Annual Congress of the Ghana Veterinary Medical Association. The Information Support Unit, Ministry of Food and Agriculture, Acca, Ghana, pp. 46-51.
2. Church G.M., Gilbert W. Genomic sequencing. Proc. Natl. Acad. Sci. U.S.A. 81:1984;1991-1995
3. Du Plessis J.L. A method for determining the Cowdria ruminantium infection rate of Amblyomma hebraeum: effects of mice injection with tick homogenates. Onderstepoort J. Vet. Res. 52:1985;55-61
4. Du Plessis J.L. The relationship between Cowdria and Ehrlichia: change in the behaviour of ehrlichial agents passed through Amblyomma hebraeum. Rev. Élev. Méd. Vét. Paystrop. 46:1993;131-143
5. Du Plessis J.L., Kümm N.A.L. The passage of Cowdria ruminantium in mice. J. S. Afr. Vet. Med. Assoc. 42:1971;217-221
6. Du Plessis J.L., Malan L. The application of the indirect fluorescent antibody test in research on heartwater. Onderstepoort J. Vet. Res. 54:1987;319-325
7. Du Plessis J.L., van Gas L., Olivier J.A., Bezuidenhout J.D. The heterogenicity of Cowdria ruminantium isolates: cross immunity and serology in sheep and pathogenicity to mice. Onderstepoort J. Vet. Res. 56:1989;195-201
8. Felsenstein J. PHYLIP - phylogeny inference package (version 3.2). Cladistics. 5:1989;164-166
9. Haig D.A. Tick-borne rickettsiosis in South Africa. Adv. Vet. Sci. 2:1952;307-325
10. Jongejan F., Uilenberg G., Franssen F.F.J., Gueye A., Nieuwenhuijs J. Antigenic differences between stocks of Cowdria ruminantium. Res. Vet. Sci. 44:1988;186-189
11. Jongejan F., Thielemans M.J.C., de Groot M., van Kooten P.J.S., van der Zeijst B.A.M. Competitive enzyme-linked immunosorbent assay for heartwater using monoclonal antibodies to a Cowdria ruminantium-specific 32 kilodalton protein. Vet. Microbiol. 28:1991;199-211
12. Logan, L.L., Endis, R.G., Birnie, E.F., Mebus, C.A., 1985. Research objectives to improve U.S. diagnostic capabilities for heartwater disease. In: Proceedings of the 89th Annual Meeting. United States Animal Health Association, Milwaukee, WI, USA.
13. Logan L.L., Holland C.A., Mebus C.A., Ristic M. Serological relationship between Cowdria ruminantium and certain Ehrlichia spp. Vet. Rec. 119:1986;458-489
14. MacKenzie, P.K.I., van Rooyen, R.E., 1981. The isolation and culture of Cowdria ruminantium in albino mice. In: Proceedings of the International Congress on Tick Biology and Control, Grahamstown, South Africa, 27-29 January, pp. 33-39.
15. Mahan S.M., Waghela S.D., McGuire T.C., Rurangirwa F.R., Wassink L.A., Barbet A.F. A cloned DNA probe for Cowdria ruminantium hybridized with eight heartwater strains and detects infected sheep. J. Clin. Microbiol. 30:1992;981-986
16. Mahan S.M., Andrew H.R., Tebele N. Immunisation of sheep against heartwater with inactivated Cowdria ruminantium. Res. Vet. Sci. 58:1995;46-49
17. Neitz A.W.H., Viljoen G.J., Bezuidenhout J.D., Oberem P.T., van Wyngaardt W., Vermeulen N.M.J. The detection of antibody to Cowdria ruminantium in serum and C. ruminantium antigen in Amblyomma hebraeum by means of an enzyme-linked immunosorbent assay. Onderstepoort J. Vet. Res. 53:1986;39-41
18. Peter T.F., Deem S.L., Barbet A.F., Norval R.A.I., Simbi B.H., Kelly P.J., Mahan S.M. Development and evaluation of PCR assay for detection of low levels of Cowdria ruminantium infection in Amblyomma ticks not detected by DNA probe. J. Clin. Microbiol. 33:1995;166-172
19. Peter T.F., Barbet A.F., Alleman A.R., Simbi B.H., Burridge M.J., Mahan S.M. Detection of the agent of heartwater, Cowdria ruminantium, in Amblyomma ticks by PCR: validation and application of the assay to field ticks. J. Clin. Microbiol. 38:2000;1539-1544
20. Saiki R.K., Bugawan T.L., Horn G.T., Mullis K.B., Ehrlich H.A. Analysis of enzymatically amplified β-globin and HLA-DQa DNA with allele-specific oligonucleotide probes. Nature. 324:1986;163-166
21. Sambrook, J., Fritsch, E.F., Maniatis, T., 1989. Molecular Cloning - A Laboratory Manual, 2nd ed. Cold Spring Harbor Laboratory Press, New York, USA.
22. Siebert P.D., Chenchik A., Kellogg D.E., Lukyanov K.A., Lukyanov S.A. An improved method for walking in uncloned genomic DNA. Nucl. Acid Res. 23:1995;1087-1088
23. Thompson J.D., Higgins D.G., Gibson T.J. CLUSTALW: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucl. Acid Res. 22:1994;4673-4680
24. Uilenberg G., Camus E., Barré N. Quelques observations sur une souche de Cowdria ruminantium isolée eb Gaudeloupe (Antilles francaises). Rev. Élev. Méd. Vét. Paystrop. 38:1985;34-42
25. Waghela S.D., Rurangirwa F.R., Mahan S.M., Yunker C.E., Crawford T.B., Barbet A.F., Burridge M.J., McGuire T.C. A cloned DNA probe identifies Cowdria ruminantium in Amblyomma variegatum ticks. J. Clin. Microbiol. 29:1991;2571-2577
26. Yunker C.E., Byrom B., Semu S. Cultivation of Cowdria ruminantium in bovine vascular endothelial cells. Kenya Vet. 12:1988;12-16
27. Yunker C.E., Mahan S.M., Waghela S.D., McGuire T.C., Rurangirwa F.R., Barbet A.F., Wassink L.A. Detection of Cowdria ruminantium by means of a DNA probe, pCS20, in infected bont ticks, Amblyomma hebraeum, the major vector of heartwater in southern Africa. Epidemiol. Infect. 110:1993;95-104
28. Zweygarth E., Josemans A.I., Horn E. Serum-free media for the in vitro cultivation of Cowdria ruminantium. Ann. NY Acad. Sci. 849:1998;307-312
29. Zweygarth E., Josemans A.I., van Strijp M.F., van Heerden H., Allsopp M.T.E.P. Isolation, propagation, and characterisation of the Kümm isolate of Cowdria ruminantium. Onderstepoort J. Vet. Res. 69:2002;147-153