The immunogenicity of recombinant and dimeric gonadotrophin-releasing hormone vaccines incorporating a T-helper epitope and GnRH or repeated GnRH units

Journal of Immunological Methods

Volumn 289, Issue 1-2, 2004, Pages 111-122

  Jinshu, Xu ^a   Jingjing, Liu ^a   Duan, Peng ^a   Zheng, Zhu ^a   Ding, Ming ^a   Jie, Wu ^a   Rongyue, Cao ^a   Zhuoyi, Hu ^a  

^a CHINA PHARMACEUTICAL UNIVERSITY   (China)

Author keywords

3,3 diaminobenzidine; Anti GnRH antibody; B epitope; DAB; Dimerization; GnRH; gonadotrophin releasing hormone; Hinge; IPTG; measles virus protein (LSEIKGVIVRLEGVAK); MVP; T helper epitope; the fragment of human IgG1 (TCPPCPAP)

Indexed keywords

ALCOHOL; ANTIGEN; ASPARAGINASE; ASPARTIC ACID; DIMER; DNA; EPITOPE; GONADORELIN DERIVATIVE; HYDROCHLORIC ACID; IMMUNOGLOBULIN G; IMMUNOGLOBULIN G1; PROLINE DERIVATIVE; PROLYLGLUTAMYLHISTIDYLTRYPTOPHYLSERYLTYROSYLGLYCYLLEUCYLARGINYLPROLYLGLYCYL GLYCYLTHREONYLCYSTEINYLPROLYLPROLYLCYSTEINYLPROLYLALANYLPROLYLLEUCYLSERYLGLUTAMY LISOLEUCYLLYSYLGLYCYLVALYLISOLEUCYLVALYLARGINYLLEUCYLGLUTAMYLGLYCYLVALYLALANYLLY SINE; RECOMBINANT PROTEIN; UNCLASSIFIED DRUG; VACCINE; VIRUS FUSION PROTEIN;

ANIMAL EXPERIMENT; ARTICLE; CARBOXY TERMINAL SEQUENCE; CELL INCLUSION; CONTROLLED STUDY; DIMERIZATION; ESCHERICHIA COLI; FEMALE; GENE CONSTRUCT; GENE EXPRESSION; GENETIC CODE; HELPER CELL; IMMUNE RESPONSE; IMMUNOGENICITY; MALE; NONHUMAN; OXIDATION; PRECIPITATION; PRIORITY JOURNAL; PROTEIN DEGRADATION; PROTEIN PURIFICATION; RAT;

AMINO ACID SEQUENCE; ANIMALS; DIMERIZATION; EPITOPES, T-LYMPHOCYTE; ESCHERICHIA COLI; FEMALE; GONADOTROPIN-RELEASING HORMONE; HUMANS; IMMUNE SERA; MALE; MOLECULAR SEQUENCE DATA; PROTEIN ENGINEERING; RATS; RECOMBINANT FUSION PROTEINS; T-LYMPHOCYTES, HELPER-INDUCER; VACCINES, SUBUNIT; VIRAL PROTEINS;

ESCHERICHIA COLI; MEASLES VIRUS;

EID: 3142613001     PISSN: 00221759     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jim.2004.04.004     Document Type: Article

References (32)

1. Barrios C., Tougne C., Polla B.S., Lambett P.H., DelGiudice G. Specificity of antibodies induced after immunization of mice with the mycobacterial heat shock protein of 65 kDa. Clin. Exp. Immunol. 98:1994;224
2. Brons N.H., Blaich A., Wiesmuller K.H., Schneider F., Jung G., Muller C.P. Hierarchic T-cell help to non-linked B-cell epitopes. Scand. J. Immunol. 44:1996;478
3. Braden T.D., Hawes B.E., Conn P.M. Synthesis of GnRH receptors by gonadotrope cell cultures. Endocrinology. 125:1989;1623
4. Carelli C., Audibert F., Gaillard J., Chedid L. Immunological castration of male mice by a totally synthetic vaccine administered in saline. Proc. Natl. Acad. Sci. U. S. A. 79:1982;5392
5. Caro-Aguilar I., Rodriguez I.A., Calvo-Calle M., Guzman F., Delavega P., Patarroyo M.E., Galinski M.R., Moreno A. Plasmodium vivax promiscuous T-helper epitopes defined and evaluated as linear peptide chimera immunogens. Infect. Immun. 70:2002;3479
6. Conn P.M., Crowley W.F. Gonadotropin-releasing hormone and its analogs. Annu. Rev. Med. 45:1994;391
7. Davis D., Davies A., Gregoriadis G. Liposomes as response. Immunopurified Lett. 14:1987;341
8. Ferro V.A. Current advances in antifertility vaccines for fertility control and noncontraceptive applications. Expert Rev. Vaccines. 1:2002;443
9. Ferro V.A., Stimson W.H. Investigation into suitable carrier molecules for use in an anti-gonadotrophin releasing hormone vaccine. Vaccine. 16:1998;1095
10. Ferro V.A., Harvey M.J., Colston A., Stimson W.H. Part II: influence of dimerization of a modified GnRH-I peptide sequence on a male antifertility vaccines. Am. J. Reprod. Immunol. 48:2002;372
11. Ghosh S., Jackson D.C. Antigenic and immunogenic properties of totally synthetic peptide-based anti-fertility vaccines. Int. Immunol. 11:1999;1103
12. Hayman W.A., Brandt E.R., Relf W.A., Cooper J., Saul A., Good M.F. Mapping the minimal murine T cell and B cell epitopes with a peptide vaccine candidate from the conserved region of the M protein of group A streptococcus. Int. Immunol. 9:1997;1723
13. Herzenberg L.A., Tokuhisa T., Hayakawa K. Epitope-specific regulation. Annu. Rev. Immun. 1:1982;609
14. Hsu C-T., Ting C-Y., Ting C-J., Chen T-Y., Lin C-P., Jacqueling W-P., Hwang J. Vaccination against gonadotropin-releasing hormone (GnRH) using toxin receptor-binding domain-conjugated GnRH repeats. Cancer Res. 60:2000;3701
15. Jacobs E., Watson S.A., Michaeli D., Ellis I.O., Robertson J.F.R. Anti-gonadotrophin releasing hormone antibodies inhibit the growth of MCF7 human breast cancer xenografts. Br. J. Cancer. 80:1999;352
16. Kessler H., Mronga S., Müller G., Moroder L., Huber R. Conformational analysis of a IgG1 hinge peptide derivative in solution determined by NMR spectroscopy and refined by restrained molecular dynamics simulations. Biopolymers. 31:1991;1189
17. Laemmli U.K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature (London). 227:1970;680
18. Lee S.J., Lee J.H., Jin H.J., Lee J.H., Ryu H.Y., Kim Y., Kong I.S., Kim K.W. A novel technique for the effective production of short peptide analogs from concatameric short peptide multimers. Mol. Cells. 10:2000;236
19. Meloen R.H. Basic aspects of immunomodulation through active immunisation. Livest. Prod. Sci. 42:1995;135
20. Moroder L., Musiol H.-J., Kocher K., Bali J.P., Schneider C.H., Guba W., Muller G., Mierke D.F., Kessler H. Synthetic immunogens: the effect of the conformational space on biological and immunological responses to dimeric hormone constructs. Eur. J. Biochem. 2:1993;325
21. Muller C.P., Ammerlaan W., Fleckenstein B., Krauss S., Kalbacher H., Schneider F., Jung G., Wiesmuller K.H. Activation of T cells by the ragged tail of MHC class II-presented peptides of the measles virus fusion protein. Int. Immunol. 8:1996;445
22. Partidos C.D., Steward M.W. Prediction and identification of a T cell epitope in the fusion protein of measles virus immunodominant in mice and humans. J. Gen. Virol. 71:1990;2099
23. Partidos C.D., Obeid O.E., Steward M.W. Antibody responses to non-immunogenic synthetic peptides induced by co-immunization with immunogenic peptides. Immunology. 77:1992;262
24. Perraut R., Lussow A.R., Gavoille S., Garraud O., Matile H., Tougne C., van Embden J., van der Zee R., Lambert P.-H., Gysin J., del Giudice G. Successful primate immunization with peptides conjugated to purified protein derivative or mycobacterial heat shock proteins in the absence of adjuvants. Clin. Exp. Immunol. 93:1993;382
25. Sad S., Rao K., Arora R., Talwar G.P., Raghupathy R. Bypass of carrier-induced epitope-specific suppression using a T-hepler epitope. Immunology. 76:1992;599
26. Sad S., Chauhan V.S., Arunan K., Raghupathy R. Synthetic gonadotrophin-releasing hormane (GnRH) vaccines incorporating GnRH and synthetic T-helper epitopes. Vaccine. 11:1993;1145
27. Schagger H., von Jagow G. Tricince-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa. Anal. Biochem. 166:1987;368
28. Schally A.V., Arimura A., Kastin A.J. Hypothalamic regulatory hormones. Science. 179:1973;341
29. Schutze M.P., Leclerc C., Jolivet M., Audidert F., Chedid L. Carrier-induced epitopic suppression, a major issue for future synthetic vaccines. J. Immunol. 135:1985;2319
30. Sosa J.M., Zhang Y., de Avila D.M., Bertrand K.P., Reeves J.J. Recombinant LHRH fusion protein suppresses estrus in heifers. J. Anim. Sci. 78:2000;1310
31. Turkstra J.A., Oonk H.B., Schaaper W.H., Meloen R.H. The role of the individual amino acids of a GnRH-tandem-dimer peptide used as an antigen for immunocastration of male piglets determined with systematic alanine replacements. Vaccine. 20:2001;406
32. Zeng W., Ghosh S., Lau Y.F., Brown L.E., Jackson D.C. Highly immunogenic and totally synthetic lipopeptides as self-adjuvanting immunocontraceptive vaccines. J. Immunol. 169:2002;4905