Methylation analysis of the promoter F of estrogen receptor α gene: Effects on the level of transcription on human osteoblastic cells

Journal of Steroid Biochemistry and Molecular Biology

Volumn 91, Issue 1-2, 2004, Pages 1-9

  Penolazzi, Letizia ^a   Lambertini, Elisabetta ^a   Giordano, Silvia ^a   Sollazzo, Vincenzo ^a   Traina, Giancarlo ^a   Del Senno, Laura ^a   Piva, Roberta ^a  

^a UNIVERSITY OF FERRARA   (Italy)

Author keywords

DNA methylation; Estrogen receptor alfa; Osteoblasts

Indexed keywords

BISULFITE; ESTROGEN RECEPTOR ALPHA; GENOMIC DNA; MESSENGER RNA;

ARTICLE; BONE CELL; BONE TISSUE; CELL STRAIN MCF 7; CORRELATION ANALYSIS; DNA METHYLATION; EXON; GENE EXPRESSION; GENE LOCATION; GENE SEQUENCE; GENETIC TRANSCRIPTION; HUMAN; HUMAN CELL; HUMAN TISSUE; OSTEOBLAST; PROMOTER REGION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SEQUENCE ANALYSIS;

ADULT; AGED; ALKALINE PHOSPHATASE; BINDING SITES; BONE AND BONES; CELL LINE, TUMOR; CPG ISLANDS; DNA METHYLATION; DNA, COMPLEMENTARY; ESTROGEN RECEPTOR ALPHA; EXONS; FEMALE; GENE EXPRESSION REGULATION; HUMANS; IMMUNOHISTOCHEMISTRY; MALE; MIDDLE AGED; MODELS, GENETIC; OSTEOBLASTS; PROMOTER REGIONS (GENETICS); PROTEIN ISOFORMS; RECEPTORS, ESTROGEN; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, MESSENGER; SULFITES; TRANSCRIPTION, GENETIC;

EID: 3142517962     PISSN: 09600760     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jsbmb.2004.02.005     Document Type: Article

References (40)

1. Nilsson S., Makela S., Treuter E., Tujague M., Thomsen J., Andersson G., Enmark E., Pettersson K., Warner M., Gustafsson J.-A. Mechanism of estrogen action. Physiol. Rev. 4:2001;1535-1565
2. Moggs J.G., Orphanides G. Estrogen receptors: orchestrators of pleiotropic cellular responses. EMBO Rep. 2:2001;775-781
3. Flouriot G., Griffin C., Kenealy M., Sonntag-Buck V., Gannon F. Differentially expressed mRNA isoforms of the human estrogen receptor-α gene are generated by alternative splicing and promoter usage. Mol. Endocrinol. 12:1998;1938-1954
4. Ordway J.M., Curran T. Methylation matters: modeling a manageable genome. Cell Growth Differ. 13:2002;149-162
5. Jones P.A., Takai D. The role of DNA methylation in mammalian epigenetics. Science. 293:2001;1068-1070
6. Wade P.A. Methyl CpG-binding proteins and transcriptional repression. Bioessays. 23:2001;1131-1137
7. Berger J., Daxenbichler G. DNA methylation of nuclear receptor genes - possible role in malignancy. J. Steroid Biochem. Mol. Biol. 80:2002;1-11
8. Post W.S., Goldschmidt-Clermont P.J., Wilhide C.C., Heldman A.W., Sussman M.S., Ouyang P., Milliken E.E., Issa J.P. Methylation of the estrogen receptor gene is associated with aging and atherosclerosis in the cardiovascular system. Cardiovasc. Res. 43:1999;985-991
9. Compston J.E. Sex steroids and bone. Physiol. Rev. 81:2001;419-447
10. Arts J., Kuiper G.G.J.M., Janssen J.M.M.F., Gustafsson J.-A., Lowik C.W.G.M., Pols H.A.P., Van Leeuwen J.P.T.M. Differential expression of estrogen receptor α and β mRNA during differentiation of human osteoblast SV-HFO cells. Endocrinology. 138:1997;5067-5070
11. Denger S., Reid G., Kos M., Flouriot G., Parsch D., Brand H., Korach K.S., Sonntag-Buck V., Gannon F. ERα gene expression in human primary osteoblasts: evidence for the expression of two receptor proteins. Mol. Endocrinol. 15:2001;2064-2077
12. Lambertini E., Penolazzi L., Giordano S., Del Senno L., Piva R. Expression of the human oestrogen receptor-alpha gene is regulated by promoter F in MG-63 osteoblastic cells. Biochem. J. 372:2003;831-839
13. Siegmund K.D., Laird P.W. Analysis of complex methylation data. Methods. 27:2002;170-178
14. Frommer M., McDonald L.E., Millar D.S., Collis C.M., Watt F., Grigg G.W., Molloy P.L., Paul C.L. A genomic sequencing protocol that yields a positive display of 5-methylcytosine residues in individual DNA strands. Proc. Natl. Acad. Sci. 89:1992;1827-1831
15. Lambertini E., Penolazzi L., Sollazzo V., Pezzetti F., de Mattei M., del Senno L., Traina G.C., Piva R. Modulation of gene expression in human osteoblasts by targeting a distal promoter region of human estrogen receptor-α gene. J. Endocrinol. 172:2002;683-693
16. Paulin R., Grigg G.W., Davey M.W., Piper A.A. Urea improves efficiency of bisulphite-mediated sequencing of 5′-methylcytosine in genomic DNA. Nucleic Acid Res. 21:1998;5009-5010
17. Ibbotson K.J., Harrod J., Gowen M., D'Souza S., Smith D.D., Winkler M.E., Derynck R., Mundy G.R. Human recombinant transforming growth factor alpha stimulates bone resorption and inhibits formation in vitro. Proc. Natl. Acad. Sci. U.S.A. 83:1986;2228-2232
18. Lambertini E., Penolazzi L., Aguiari G., del Senno L., Pezzetti F., Sollazzo V., Piva R. Osteoblastic differentiation induced by transcription factor decoy against human estrogen receptor α gene. Biochem. Biophys. Res. Commun. 292:2002;761-770
19. Denhardt D.T., Guo X. Osteopontin: a protein with diverse functions. FASEB J. 7:1993;1475-1482
20. Sasaki M., Tanaka Y., Perinchery G., Dharia A., Kotcherguina I., Fujimoto S., Dahiya R. Methylation and inactivation of estrogen, progesterone, and androgen receptors in prostate cancer. J. Natl. Cancer Inst. 94:2002;384-390
21. Sasaki M., Dharia A., Oh B.R., Tanaka Y., Fujimoto S., Dahiya R. Progesterone receptor B gene inactivation and CpG hypermethylation in human uterine endometrial cancer. Cancer Res. 61:2001;97-102
22. Sasaki M., Kaneuchi M., Fujimoto S., Tanaka Y., Dahiya R. Hypermethylation can selectively silence multiple promoters of steroid receptors in cancers. Mol. Cell. Endocrinol. 202:2003;201-207
23. Liu Z.J., Maekawa M. Gene promoter methylation - a potential cancer marker. Adv. Clin. Pathol. 6:2002;45-49
24. Jaenisch R., Bird A. Epigenetic regulation of gene expression: how the genome integrates intrinsic and environmental signals. Nat. Genet. 33:2003;245-254
25. Yuasa Y. DNA methylation in cancer and ageing. Mech. Ageing Dev. 123:2002;1649-1654
26. Ahuja N., Li Q., Mohan A.L., Baylin S.B., Issa J.P. Aging and DNA methylation in colorectal mucosa and cancer. Cancer Res. 58:1998;5489-5494
27. Yoshid T., Eguchi H., Nakachi K., Tanimoto K., Higashi Y., Suemasu K., Iino Y., Morishita Y., Hayashi S.I. Distinct mechanisms of loss of estrogen receptor α gene expression in human breast cancer: methylation of the gene and alteration of trans-acting factors. Carcinogenesis. 21:2000;2193-2201
28. Ottaviano Y.L., Issa J.P., Parl F.F., Smith H.S., Baylin S.B., Davidson N.E. Methylation of the estrogen receptor gene CpG island marks loss of estrogen receptor expression in human breast cancer cells. Cancer Res. 54:1994;2552-2555
29. Sasaki M., Kotcherguina L., Dharia A., Fujimoto S., Dahiya R. Cytosine-phosphoguanine methylation of estrogen receptors in endometrial cancer. Cancer Res. 61:2001;3262-3266
30. Issa J.P., Ottaviano Y.L., Celano P., Hamilton S.R., Davidson N.E., Baylin S.B. Methylation of the oestrogen receptor CpG island links ageing and neoplasia in human colon. Nat. Genet. 7:1994;536-540
31. Ying A.K., Hassanain H.H., Roos C.M., Smiraglia D.J., Issa J.P.J., Michler R.E., Caligiuri M., Plass C., Goldschmidt-Clermont P.J. Methylation of the estrogen receptor-α gene promoter is selectively increased in proliferating human aortic smooth muscle cells. Cardiovasc. Res. 46:2000;172-179
32. Kos M., Reid G., Denger S., Gannon F. Minireview: genomic organization of the human ERα gene promoter region. Mol. Endocrinol. 15:2001;2057-2063
33. Villagra A., Gutierrez J., Paredes R., Sierra J., Puchi M., Imschenetzky M., Wijnen Av A., Lian J., Stein G., Stein J., Montecino M. Reduced CpG methylation is associated with transcriptional activation of the bone-specific rat osteocalcin gene in osteoblasts. J. Cell. Biochem. 85:2002;112-122
34. al-Azzawi F., Wahab M. Estrogen and colon cancer: current issues. Climacteric. 5:2002;3-14
35. Li Q., Kopecky K.J., Mohan A., Willman C.L., Appelbaum F.R., Weick J.K., Issa J.P. Estrogen receptor methylation is associated with improved survival in adult acute myeloid leukemia. Clin. Cancer Res. 5:1999;1077-1084
36. Liu Z.J., Maekawa M., Horii T., Morita M. The multiple promoter methylation profile of PR gene and ER alpha gene in tumor cell lines. Life Sci. 73:2003;1963-1972
37. Esteller M., Corn P.G., Baylin S.B., Herman J.G. A gene hypermethylation profile of human cancer. Cancer Res. 61:2001;3225-3229
38. Baylin S.B., Herman J.G. DNA hypermethylation in tumorigenesis: epigenetics joins genetics. Trends Genet. 16:2000;168-174
39. Maekawa M., Sugano K., Ushiama M., Fukayama N., Nomoto K., Kashiwabara H., Fujita S., Kakizoe T. Heterogeneity of DNA methylation status analyzed by bisulfite-PCR-SSCP and correlation with clinico-pathological characteristics in colorectal cancer. Clin. Chem. Lab. Med. 39:2001;121-128
40. Macaluso M., Cinti C., Russo G., Russo A., Giordano A. pRb2/p130-E2F4/5-HDAC1-SUV39H1-p300 and pRb2/p130-E2F4/5-HDAC1-SUV39H1-DNMT1 multimolecular complexes mediate the transcription of estrogen receptor-alpha in breast cancer. Oncogene. 22:2003;3511-3517