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Volumn 349, Issue 1, 2006, Pages 62-71

Protein/RNA coextraction and small two-dimensional polyacrylamide gel electrophoresis for proteomic/gene expression analysis of renal cancer biopsies

Author keywords

2 DE; Biopsy; Kidney; MALDI TOF; Q TOF; Real time PCR

Indexed keywords

BIOPSY; DESORPTION; DISEASES; ELECTROPHORESIS; GELS; GENES; MASS SPECTROMETRY; POLYMERASE CHAIN REACTION; RNA; TISSUE;

EID: 31144465975     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2005.10.020     Document Type: Article
Times cited : (17)

References (29)
  • 1
    • 0037347236 scopus 로고    scopus 로고
    • Evaluation of nonionic and zwitterionic detergents as membrane protein solubilizers in two-dimensional electrophoresis
    • S. Luche, V. Santoni, and T. Rabilloud Evaluation of nonionic and zwitterionic detergents as membrane protein solubilizers in two-dimensional electrophoresis Proteomics 3 2003 249 253
    • (2003) Proteomics , vol.3 , pp. 249-253
    • Luche, S.1    Santoni, V.2    Rabilloud, T.3
  • 2
    • 0037709388 scopus 로고    scopus 로고
    • Optimizing protein solubility for two-dimensional gel electrophoresis analysis of human myocardium
    • B.A. Stanley, I. Neverova, H.A. Brown, and J.E. Van Eyk Optimizing protein solubility for two-dimensional gel electrophoresis analysis of human myocardium Proteomics 3 2003 815 820
    • (2003) Proteomics , vol.3 , pp. 815-820
    • Stanley, B.A.1    Neverova, I.2    Brown, H.A.3    Van Eyk, J.E.4
  • 4
    • 2442597883 scopus 로고    scopus 로고
    • Convenient and versatile subcellular extraction procedure that facilitates classical protein expression profiling and functional protein analysis
    • A. Abdolzade-Bavil, S. Hayes, L. Goretzki, M. Kroger, J. Anders, and R. Hendriks Convenient and versatile subcellular extraction procedure that facilitates classical protein expression profiling and functional protein analysis Proteomics 4 2004 1397 1405
    • (2004) Proteomics , vol.4 , pp. 1397-1405
    • Abdolzade-Bavil, A.1    Hayes, S.2    Goretzki, L.3    Kroger, M.4    Anders, J.5    Hendriks, R.6
  • 5
    • 0037265724 scopus 로고    scopus 로고
    • Extraction of proteins from plant tissues for two-dimensional electrophoresis analysis
    • P. Giavalisco, E. Nordhoff, H. Lehrach, J. Gobom, and J. Klose Extraction of proteins from plant tissues for two-dimensional electrophoresis analysis Electrophoresis 24 2003 207 216
    • (2003) Electrophoresis , vol.24 , pp. 207-216
    • Giavalisco, P.1    Nordhoff, E.2    Lehrach, H.3    Gobom, J.4    Klose, J.5
  • 6
    • 0345873477 scopus 로고    scopus 로고
    • An optimized method for the isolation and identification of membrane proteins
    • I. Lehner, M. Niehof, and J. Borlak An optimized method for the isolation and identification of membrane proteins Electrophoresis 24 2003 1795 1808
    • (2003) Electrophoresis , vol.24 , pp. 1795-1808
    • Lehner, I.1    Niehof, M.2    Borlak, J.3
  • 7
    • 0042861598 scopus 로고    scopus 로고
    • Proteomic changes in renal cancer and co-ordinate demonstration of both the glycolytic and mitochondrial aspects of the Warburg effect
    • R.D. Unwin, R.A. Craven, P. Harnden, S. Hanrahan, N. Totty, M. Knowles, I. Eardley, P.J. Selby, and R.E. Banks Proteomic changes in renal cancer and co-ordinate demonstration of both the glycolytic and mitochondrial aspects of the Warburg effect Proteomics 3 2003 1620 1632
    • (2003) Proteomics , vol.3 , pp. 1620-1632
    • Unwin, R.D.1    Craven, R.A.2    Harnden, P.3    Hanrahan, S.4    Totty, N.5    Knowles, M.6    Eardley, I.7    Selby, P.J.8    Banks, R.E.9
  • 10
    • 0036120616 scopus 로고    scopus 로고
    • Laser capture microdissection and two-dimensional polyacrylamide gel electrophoresis: Evaluation of tissue preparation and sample limitations
    • R.A. Craven, N. Totty, P. Harnden, P.J. Selby, and R.E. Banks Laser capture microdissection and two-dimensional polyacrylamide gel electrophoresis: Evaluation of tissue preparation and sample limitations Am. J. Pathol. 160 2002 815 822
    • (2002) Am. J. Pathol. , vol.160 , pp. 815-822
    • Craven, R.A.1    Totty, N.2    Harnden, P.3    Selby, P.J.4    Banks, R.E.5
  • 11
    • 0036857346 scopus 로고    scopus 로고
    • Renal cell carcinoma: Handling and treatment
    • C. Sarto, C. Valsecchi, and P. Mocarelli Renal cell carcinoma: handling and treatment Proteomics 2 2002 1627 1629
    • (2002) Proteomics , vol.2 , pp. 1627-1629
    • Sarto, C.1    Valsecchi, C.2    Mocarelli, P.3
  • 12
    • 0037416788 scopus 로고    scopus 로고
    • Technique for obtaining highly enriched, quiescent immature Langerhans cells suitable for ex vivo assays
    • I. Tchou, O. Sabido, C. Lambert, L. Misery, O. Garraud, and C. Genin Technique for obtaining highly enriched, quiescent immature Langerhans cells suitable for ex vivo assays Immunol. Lett. 86 2003 7 14
    • (2003) Immunol. Lett. , vol.86 , pp. 7-14
    • Tchou, I.1    Sabido, O.2    Lambert, C.3    Misery, L.4    Garraud, O.5    Genin, C.6
  • 13
    • 0034460725 scopus 로고    scopus 로고
    • Characterization of endothelial cells isolated by human meningiomas
    • F. Giordano, and S. Mitola Characterization of endothelial cells isolated by human meningiomas J. Neurosurg. Sci. 44 2000 177 185
    • (2000) J. Neurosurg. Sci. , vol.44 , pp. 177-185
    • Giordano, F.1    Mitola, S.2
  • 14
    • 0031888036 scopus 로고    scopus 로고
    • High throughput protein characterization by automated reverse-phase chromatography/electrospray tandem mass spectrometry
    • A. Ducret, I. Van Oostveen, J.K. Eng, J.R. Yates III, and R. Aebersold High throughput protein characterization by automated reverse-phase chromatography/electrospray tandem mass spectrometry Protein Sci. 7 1998 706 719
    • (1998) Protein Sci. , vol.7 , pp. 706-719
    • Ducret, A.1    Van Oostveen, I.2    Eng, J.K.3    Yates III, J.R.4    Aebersold, R.5
  • 15
    • 0036674269 scopus 로고    scopus 로고
    • Large-scale proteomic analysis of the human spliceosome
    • J. Rappsilber, U. Ryder, A.I. Lamond, and M. Mann Large-scale proteomic analysis of the human spliceosome Genome Res. 12 2002 1231 1245
    • (2002) Genome Res. , vol.12 , pp. 1231-1245
    • Rappsilber, J.1    Ryder, U.2    Lamond, A.I.3    Mann, M.4
  • 16
    • 0035106351 scopus 로고    scopus 로고
    • Large-scale analysis of the yeast proteome by multidimensional protein identification technology
    • M.P. Washburn, D. Wolters, and J.R. Yates III Large-scale analysis of the yeast proteome by multidimensional protein identification technology Nat. Biotechnol. 19 2001 242 247
    • (2001) Nat. Biotechnol. , vol.19 , pp. 242-247
    • Washburn, M.P.1    Wolters, D.2    Yates III, J.R.3
  • 17
    • 85099524713 scopus 로고    scopus 로고
    • Proteomic analysis of proteins expressed by Helicobacter pylori under oxidative stress
    • (in press).
    • M.H. Chuang, M.S. Wu, J.T. Lin, S.H. Chiou, Proteomic analysis of proteins expressed by Helicobacter pylori under oxidative stress, Proteomics (in press).
    • Proteomics
    • Chuang, M.H.1    Wu, M.S.2    Lin, J.T.3    Chiou, S.H.4
  • 18
    • 19344362719 scopus 로고    scopus 로고
    • Two-dimensional gel electrophoresis characterization of the mouse leukocyte proteome using a tri-reagent for protein extraction
    • N.I. Lenchik, D.M. Desiderio, and I.C. Gerling Two-dimensional gel electrophoresis characterization of the mouse leukocyte proteome using a tri-reagent for protein extraction Proteomics 5 2005 2202 2209
    • (2005) Proteomics , vol.5 , pp. 2202-2209
    • Lenchik, N.I.1    Desiderio, D.M.2    Gerling, I.C.3
  • 19
    • 1842430894 scopus 로고    scopus 로고
    • A combination of proteomics, principal component analysis, and transcriptomics is a powerful tool for the identification of biomarkers for macrophage maturation in the U937 cell line
    • K.C. Verhoeckx, S. Bijlsma, E.M. de Groene, R.F. Witkamp, J. van der Greef, and R.J. Rodenburg A combination of proteomics, principal component analysis, and transcriptomics is a powerful tool for the identification of biomarkers for macrophage maturation in the U937 cell line Proteomics 4 2004 1014 1028
    • (2004) Proteomics , vol.4 , pp. 1014-1028
    • Verhoeckx, K.C.1    Bijlsma, S.2    De Groene, E.M.3    Witkamp, R.F.4    Van Der Greef, J.5    Rodenburg, R.J.6
  • 20
    • 0023277545 scopus 로고
    • Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction
    • P. Chomczynski, and N. Sacchi Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction Anal. Biochem. 162 1987 156 159
    • (1987) Anal. Biochem. , vol.162 , pp. 156-159
    • Chomczynski, P.1    Sacchi, N.2
  • 22
    • 0028603364 scopus 로고
    • Sample application by in-gel rehydration improves the resolution of two-dimensional electrophoresis with immobilized pH gradients in the first dimension
    • T. Rabilloud, C. Valette, and J.J. Lawrence Sample application by in-gel rehydration improves the resolution of two-dimensional electrophoresis with immobilized pH gradients in the first dimension Electrophoresis 15 1994 1552 1558
    • (1994) Electrophoresis , vol.15 , pp. 1552-1558
    • Rabilloud, T.1    Valette, C.2    Lawrence, J.J.3
  • 25
    • 0031214574 scopus 로고    scopus 로고
    • Identification of proteins by matrix-assisted laser desorption/ ionisation-mass spectrometry following in-gel digestion in low-salt, nonvolatile buffer and simplified peptide recovery
    • M. Fountoulakis, and H. Langen Identification of proteins by matrix-assisted laser desorption/ionisation-mass spectrometry following in-gel digestion in low-salt, nonvolatile buffer and simplified peptide recovery Anal. Biochem. 250 1997 153 156
    • (1997) Anal. Biochem. , vol.250 , pp. 153-156
    • Fountoulakis, M.1    Langen, H.2
  • 26
    • 0034213595 scopus 로고    scopus 로고
    • ProFound: An expert system for protein identification using mass spectrometric peptide mapping information
    • W. Zhang, and B.T. Chait ProFound: an expert system for protein identification using mass spectrometric peptide mapping information Anal. Chem. 72 2000 2482 2489
    • (2000) Anal. Chem. , vol.72 , pp. 2482-2489
    • Zhang, W.1    Chait, B.T.2
  • 27
    • 17344392308 scopus 로고    scopus 로고
    • A new mathematical model for relative quantification in real-time RT-PCR
    • M.W. Pfaffl A new mathematical model for relative quantification in real-time RT-PCR Nucleic Acids Res. 29 2001 2002 2007
    • (2001) Nucleic Acids Res. , vol.29 , pp. 2002-2007
    • Pfaffl, M.W.1


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