Method for rapid conjugation of recombinant photoprotein aequorin with streptavidin and application as a universal detection reagent for binding assays

Analytica Chimica Acta

Volumn 558, Issue 1-2, 2006, Pages 267-273

  Zerefos, Panayotis G ^a   Ioannou, Penelope C ^a   Christopoulos, Theodore K ^b,c  

^a UNIVERSITY OF ATHENS   (Greece)

^b UNIVERSITY OF PATRAS   (Greece)

^c INSTITUTE OF CHEMICAL ENGINEERING SCIENCES   (Greece)

Author keywords

Aequorin; Affinity separation; Bioluminescence; Conjugation; Hybridization; Streptavidin

Indexed keywords

ANTIBODIES; BIOASSAY; BIOLUMINESCENCE; DNA; MACROMOLECULES; MOLECULAR BIOLOGY; PHOTOCHEMICAL REACTIONS; RNA;

AEQUORIN; AFFINITY SEPARATION; CONJUGATION; HYBRIDIZATION; STREPTAVIDIN;

PROTEINS;

AEQUORIN; HEXAHISTIDINE; HISTIDINE DERIVATIVE; MOLECULAR MARKER; STREPTAVIDIN; UNCLASSIFIED DRUG;

AFFINITY CHROMATOGRAPHY; ARTICLE; BINDING ASSAY; CONJUGATION; DNA HYBRIDIZATION; MOLECULAR RECOGNITION; PRIORITY JOURNAL; PROTEIN PURIFICATION; SIGNAL DETECTION;

EID: 31044453183     PISSN: 00032670     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.aca.2005.10.064     Document Type: Article

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