Native structure and degradation pattern of silk sericin studied by 13C NMR spectroscopy

Macromolecules

Volumn 39, Issue 1, 2006, Pages 6-8

  Teramoto, Hidetoshi ^a,b   Kakazu, Aya ^b   Asakura, Tetsuo ^b  

^a NATIONAL INSTITUTE OF AGROBIOLOGICAL SCIENCES   (Japan)

^b TOKYO UNIVERSITY OF AGRICULTURE AND TECHNOLOGY   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

ELECTROPHORESIS; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY; PROTEINS; PYROLYSIS; SPINNING (FIBERS); STRUCTURAL ANALYSIS;

SERICIN; SERICIN HOPE; SILKWORM;

SILK;

PYROLYSIS; SILK;

EID: 30944438539     PISSN: 00249297     EISSN: None     Source Type: Journal    
DOI: 10.1021/ma0521147     Document Type: Article

References (29)

1. Fedič, R.; Zurovec, M.; Sehnal, F. J. Insect Biotechnol. Sericol. 2002, 71, 1-15.
2. Craig, C. L.; Riekel, C. Comp. Biochem. Physiol. B 2002, 133, 493-507.
3. Sinohara, H. Comp. Biochem. Physiol. 1979, 63B, 87-91.
4. Gamo, T. Biochem. Genet. 1982, 20, 165-177.
5. Garel, A.; Deleage, G.; Prudhomme, J. C. Insect Biochem. Mol. Biol. 1997, 27, 469-477.
6. Takasu, Y.; Yamada, H.; Tsubouchi, K. Biosci. Biotechnol. Biochem. 2002, 66, 2715-2718.
7. Voegeli, R.; Meier, J.; Blust, R. Cosmet. Toiletries 1993, 108, 101-108.
8. Teramoto, H.; Nakajima, K.; Takabayashi, C. Biomacromolecules 2004, 5, 1392-1398.
9. Takeuchi, A.; Ohtsuki, C.; Miyazaki, T.; Tanaka, H.; Yamazaki, M.; Tanihara, M. J. Biomed. Mater. Res. 2003, 65A, 283-289.
10. Tsubouchi, K.; Igarashi, Y.; Takasu, Y.; Yamada, H. Biosci. Biotechnol. Biochem. 2005, 69, 403-405.
11. Zhang, Y. Q. Biotechnol. Adv. 2002, 20, 91-100.
12. Panilaitis, B.; Altman, G. H.; Chen, J.; Jin, H.-J.; Karageorgiou, V.; Kaplan, D. L. Biomaterials 2003, 24, 3079-3085.
13. Teramoto, H.; Nakajima, K.; Takabayashi, C. Biosci. Biotechnol. Biochem. 2005, 69, 845-847.
14. Yamamoto, T.; Mase, K.; Miyajima, T.; Hara, K. Japanese Patent No. 3374177.
15. Yamamoto, T.; Miyajima, T.; Mase, K.; Iizuka, T. In Annual Report 2002; National Institute of Agrobiological Sciences: Tsukuba, Japan, 2002; pp 24-25.
16. Asakura, T.; Watanabe, Y.; Uchida, A.; Minagawa, H. Macromolecules 1984, 17, 1075-1081.
17. Asakura, T.; Watanabe, Y.; Itoh, T. Macromolecules 1984, 17, 2421-2426.
18. Howarth, O. W.; Lilley, D. M. J. Prog. Nucl. Magn. Reson. Spectrosc. 1978, 12, 1-40.
19. Teramoto, H.; Miyazawa, M. J. Insect Biotechnol. Sericol. 2003, 72, 157-162.
20. Yamane, T.; Umemura, K.; Nakazawa, Y.; Asakura, T. Macromolecules 2003, 36, 6766-6772.
21. Kataoka, K.; Uematsu, I. Kobunshi Ronbunshu 1977, 34, 37-41 (in Japanese).
22. Zhang, Y. Q.; Tao, M. L.; Shen, W. D.; Zhou, Y. Z.; Ding, Y.; Ma, Y.; Zhou, W. L. Biomaterials 2004, 25, 3751-3759.
23. 13C NMR spectrum of the regenerated sericin solution (Figure 3a) exhibited much smaller peaks assignable to Glu, Phe, and Tyr residues than native sericin solution (Figure la), showing that low molecular weight impurities can be removed during regeneration process. The Cα and Cβ chemical shifts of major amino acids in the regenerated sericin solution were in good agreement with those of native sericin solution, which demonstrated that sericin remained in a largely random coil structure after regeneration from the cocoon.
24. Tomizawa, H.; Yamada, H.; Imoto, T. Biochemistry 1994, 33, 13032-13037.
25. Frare, E.; Polverino de Laureto, P.; Zurdo, J.; Dobson, C. M.; Fontana, A. J. Mol. Biol. 2004, 340, 1153-1165.
26. Hosia, W.; Griffiths, W. J.; Johansson, J. J. Mass Spectrom. 2005, 40, 142-145.
27. Okamoto, H.; Ishikawa, E.; Suzuki, Y. J. Biol. Chem. 1982, 257, 15192-15199.
28. Huang, J.; Valluzzi, R.; Bini, E.; Vernaglia, B.; Kaplan, D. L. J. Biol. Chem. 2003, 278, 46117-46123.
29. Teramoto, H.; Miyazawa, M. Biomacromolecules 2005, 6, 2049-2057.