Fluorescent antibody-viability staining and β-glucuronidase assay as rapid methods for monitoring Escherichia coli viability in coastal marine waters

Journal of Immunoassay and Immunochemistry

Volumn 27, Issue 1, 2006, Pages 1-13

  Caruso, G ^a   De Pasquale, F ^a   Mancuso, M ^a   Zampino, D ^a   Crisafi, E ^a  

^a CNR   (Italy)

Author keywords

Glucuronidase; Escherichia coli; Faecal pollution; Fluorescent antibody; Seawater monitoring; Viability

Indexed keywords

BETA GLUCURONIDASE; SEA WATER;

ARTICLE; BACTERIAL CELL; BACTERIUM CONTAMINATION; BACTERIUM CULTURE; BACTERIUM DETECTION; ENZYME ACTIVATION; ENZYME ASSAY; ESCHERICHIA COLI; FECES; FLUORESCENT ANTIBODY TECHNIQUE; MARINE ENVIRONMENT; MICROSCOPY; PRIORITY JOURNAL; QUALITY CONTROL; SEROTYPE; STAINING; STATISTICAL SIGNIFICANCE; URBAN AREA; WATER CONTAMINATION;

BACTERIOLOGICAL TECHNIQUES; BIOLOGICAL ASSAY; CELL SURVIVAL; COLONY COUNT, MICROBIAL; ENVIRONMENTAL MONITORING; ESCHERICHIA COLI; FECES; FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT; GLUCURONIDASE; SEAWATER; STAINING AND LABELING; WATER MICROBIOLOGY;

EID: 30744471677     PISSN: 15321819     EISSN: 15324230     Source Type: Journal    
DOI: 10.1080/15321810500403599     Document Type: Article

References (19)

1. Sartory, D.P.; Watkins, J. Conventional culture for water quality assessment: is there a future? J. Appl. Microbiol. Symp. Suppl. 1999, 85 (26), 225S-233S.
2. Caruso, G.; Denaro, R.; Genovese, M.; Giuliano, L.; Mancuso, M.; Yakimov, M. New methodological strategies for detecting bacterial indicators. Chem. Ecol. 2004, 20 (3), 167-181.
3. Roszack, D.B.; Colwell, R.R. Metabolic activity of bacterial cells enumerated by Direct Viable Count. Appl. Environ. Microbiol. 1987, 53, 2889-2893.
4. Rozen, Y.; Belkin, S. Survival of enteric bacteria in seawater. FEMS Microbiol. Rev. 2001, 725, 1-17.
5. Xu, H.S.; Roberts, N.; Singleton, F.L.; Attwell, R.W.; Grimes, D.J.; Colwell, R.R. Survival and viability of nonculturable Escherichia coli and Vibrio cholerae in the estuarine and marine environment. Microb. Ecol. 1982, 8, 313-323.
6. Lopez-Amoros, R.; Comas, J.; Vives-Rego, J. Flow cytometric assessment of Escherichia coli and Salmonella typhimurium starvation-survival in seawater using Rhodamine 123, propidium iodide, and oxonol. Appl. Environ. Microbiol. 1995, 61, 2521-2526.
7. Muela, A.; Arana, I.; Justo, J.I.; Seco, C.; Barcina, I. Changes in DNA content and cellular death during a starvation-survival process of Escherichia coli in river water. Microb. Ecol. 1999, 37, 62-69.
8. Ericsson, M.; Hanstorp, D.; Hagberg, P.; Enger, J.; Nystrom, T. Sorting out bacterial viability with optical tweezers. J. Bacteriol. 2000, 182, 5551-5555.
9. Petit, M.; George, I.; Servais, P. Survival of Escherichia coli in freshwater: β-D-glucuronidase activity measurements and characterization of cellular states. Can. J. Microbiol. 2000, 46, 679-684.
10. Ohtomo, R.; Saito, M. Increase in the culturable cell number of Escherichia coli during recovery from saline stress: possible implication for resuscitation from the VBNC state. Microb. Ecol. 2001, 42, 208-214.
11. Pommepuy, M.; Butin, M.; Derrien, A.; Gourmelon, M.; Colwell, R.R.; Cormier, M. Retention of enteropathogenicity by viable but nonculturable Escherichia coli exposed to seawater and sunlight. Appl. Environ. Microbiol. 1996, 62, 4621-4626.
12. Caruso, G.; Zaccone, R.; Monticelli, L.; Crisafi, E.; Zampino, D. Bacterial pollution of Messina coastal waters: a one year study. Microbiologica 2000, 23, 297-304.
13. American Public Health Association. Standard methods for the examination of water and waste water, 1992, 18th Ed.; American Public Health Association: Washington, DC, 1992.
14. Caruso, G.; Crisafi, E.; Mancuso, M. Development of an enzyme assay for rapid assessment of Escherichia coli in seawaters. J. Appl. Microbiol. 2002, 93 (4), 548-556.
15. Caruso, G.; Crisafi, E.; Mancuso, M. Immunofluorescence detection of Escherichia coli in seawater: a comparison of various commercial antisera. J. Immunoassay Immunochem. 2002, 23 (4), 479-496.
16. Volterra, L.; Garizio, M. Limiti delle determinazioni microbiologiche ambientali a carattere sanitario: la colimetria. Biologi Italiani 1997, 8/97, 28-34.
17. Weichart, D. How viable is the VBNC hypothesis? Logical Biol. 2000, 1, 21-24.
18. Caruso, G.; Crisafi, E.; Mancuso, M. Coastal monitoring by MUG assay to the detection of seawater pollution. In XXXVIII Congrès CIESM; Proceedings of the Congress, Barcelona, Spain, Jun 7-11, 2004; CIESM: Monaco, 2004, 269.
19. Caruso, G.; Mancuso, M.; Crisafi, E. Combined fluorescent antibody assay and viability staining for the assessment of the physiological states of Escherichia coli in seawaters. J. Appl. Microbiol. 2003, 95 (2), 225-233.