Evaluation of the LightCycler Staphylococcus MGRADE kits on positive blood cultures that contained gram-positive cocci in clusters

Journal of Clinical Microbiology

Volumn 43, Issue 12, 2005, Pages 6144-6146

  Shrestha, Nabin K ^a   Tuohy, Marion J ^a   Padmanabhan, Ravindran A ^a   Hall, Gerri S ^a   Procop, Gary W ^a  

^a LERNER RESEARCH INSTITUTE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; BACTERIUM IDENTIFICATION; BLOOD CULTURE; COAGULASE NEGATIVE STAPHYLOCOCCUS; CONTROLLED STUDY; DEVICE; EVALUATION; GRAM POSITIVE COCCI; INOCULATION; NONHUMAN; PRIORITY JOURNAL; REAL TIME POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; STAPHYLOCOCCUS AUREUS;

BACTERIAL TYPING TECHNIQUES; BLOOD; COAGULASE; CULTURE MEDIA; DNA, BACTERIAL; GRAM-POSITIVE COCCI; HUMANS; POLYMERASE CHAIN REACTION; REAGENT KITS, DIAGNOSTIC; SENSITIVITY AND SPECIFICITY; SPECIES SPECIFICITY; STAPHYLOCOCCUS; STAPHYLOCOCCUS AUREUS;

BACTERIA (MICROORGANISMS); POSIBACTERIA; STAPHYLOCOCCUS; STAPHYLOCOCCUS AUREUS;

EID: 30744453574     PISSN: 00951137     EISSN: None     Source Type: Journal    
DOI: 10.1128/JCM.43.12.6144-6146.2005     Document Type: Article

References (13)

1. Carroll, K. C., R. B. Leonard, P. L. Newcomb-Gayman, and D. R. Hillyard. 1996. Rapid detection of the staphylococcal mecA gene from BACTEC blood culture bottles by the polymerase chain reaction. Am. J. Clin. Pathol. 106:600-605.
2. Lem, P., J. Spiegelman, B. Toye, and K. Ramotar. 2001. Direct detection of mecA, nuc and 16S rRNA genes in BacT/Alert blood culture bottles. Diagn. Microbiol. Infect. Dis. 41:165-168.
3. Louie, L., J. Goodfellow, P. Mathieu, A. Glatt, M. Louie, and A. E. Simor. 2002. Rapid detection of methicillin-resistant staphylococci from blood culture bottles by using a multiplex PCR assay. J. Clin. Microbiol. 40:2786-2790.
4. Oliveira, K., G. W. Procop, D. Wilson, J. Coull, and H. Stender. 2002. Rapid identification of Staphylococcus aureus directly from blood cultures by fluorescence in situ hybridization with peptide nucleic acid probes. J. Clin. Microbiol. 40:247-251.
5. Palomares, C., M. J. Torres, A. Torres, J. Aznar, and J. C. Palomares. 2003. Rapid detection and identification of Staphylococcus aureus from blood culture specimens using real-time fluorescence PCR. Diagn. Microbiol. Infect. Dis. 45:183-189.
6. Paule, S. M., A. C. Pasquariello, R. B. Thomson, Jr., K. L. Kaul, and L. R. Peterson. 2005. Real-time PCR can rapidly detect methicillin-susceptible and methicillin-resistant Staphylococcus aureus directly from positive blood culture bottles. Am. J. Clin. Pathol. 124:404-407.
7. Reischl, U., H. J. Linde, M. Metz, B. Leppmeier, and N. Lehn. 2000. Rapid identification of methicillin-resistant Staphylococcus aureus and simultaneous species confirmation using real-time fluorescence PCR. J. Clin. Microbiol. 38:2429-2433.
8. Sakai, H., G. W. Procop, N. Kobayashi, D. Togawa, D. A. Wilson, L. Borden, V. Krebs, and T. W. Bauer. 2004. Simultaneous detection of Staphylococcus aureus and coagulase-negative staphylococci in positive blood cultures by real-time PCR with two fluorescence resonance energy transfer probe sets. J. Clin. Microbiol. 42:5739-5744.
9. Shrestha, N. K., M. J. Tuohy, G. S. Hall, C. M. Isada, and G. W. Procop. 2002. Rapid identification of Staphylococcus aureus and the mecA gene from BacT/ALERT blood culture bottles by using the LightCycler system. J. Clin. Microbiol. 40:2659-2661.
10. Tan, T. Y., S. Corden, R. Barnes, and B. Cookson. 2001. Rapid identification of methicillin-resistant Staphylococcus aureus from positive blood cultures by real-time fluorescence PCR. J. Clin. Microbiol. 39:4529-4531.
11. Turenne, C. Y., E. Witwicki, D. J. Hoban, J. A. Karlowsky, and A. M. Kabani. 2000. Rapid identification of bacteria from positive blood cultures by fluorescence-based PCR-single-strand conformation polymorphism analysis of the 16S rRNA gene. J. Clin. Microbiol. 38:513-520.
12. Weinstein, M. P., M. L. Towns, S. M. Quartey, S. Mirrett, L. G. Reimer, G. Parmigiani, and L. B. Reller. 1997. The clinical significance of positive blood cultures in the 1990s: a prospective comprehensive evaluation of the microbiology, epidemiology, and outcome of bacteremia and fungemia in adults. Clin. Infect. Dis. 24:584-602.
13. Wellinghausen, N., B. Wirths, A. R. Franz, L. Karolyi, R. Marre, and U. Reischl. 2004. Algorithm for the identification of bacterial pathogens in positive blood cultures by real-time LightCycler polymerase chain reaction (PCR) with sequence-specific probes. Diagn. Microbiol. Infect. Dis. 48:229-241.