Exposure of phage T7 to simulated space environment: The effect of vacuum and UV-C radiation

Journal of Photochemistry and Photobiology B: Biology

Volumn 82, Issue 2, 2006, Pages 94-104

  Hegedüs, Márton ^a   Kovács, Gáspár ^b   Módos, Károly ^a   Rontó, Györgyi ^b   Lammer, Helmut ^c   Panitz, Corinna ^d   Fekete, Andrea ^a  

^a SEMMELWEIS UNIVERSITY   (Hungary)

^b RESEARCH INSTITUTE FOR TECHNICAL PHYSICS AND MATERIALS SCIENCE   (Hungary)

^c Austrian Academy of Sciences ^*  (Austria)

^d GERMAN AEROSPACE CENTER DLR   (Germany)

Author keywords

Bacteriophage; Damage; DNA; QPCR; Space simulation; Thin layer; UV radiation; Vacuum

Indexed keywords

VIRUS DNA;

ABSORPTION SPECTROSCOPY; ARTICLE; BACTERIOPHAGE T7; CONTROLLED STUDY; COSMIC RADIATION; DEHYDRATION; DNA DAMAGE; DNA ISOLATION; ELECTROPHORESIS; FILM; INTERNATIONAL COOPERATION; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; RADIATION ABSORPTION; RADIATION EXPOSURE; SAMPLING; SIMULATION; SPACE; ULTRAVIOLET C RADIATION;

BACTERIOPHAGE T7; DNA DAMAGE; DNA, VIRAL; EXTRATERRESTRIAL ENVIRONMENT; ULTRAVIOLET RAYS; VACUUM;

EID: 30744451675     PISSN: 10111344     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jphotobiol.2005.09.002     Document Type: Article

References (46)

1. W. Schulte, P. Baglioni, R. Demets, Exobiology research platform, in: Proceedings of the First European Workshop for Exo-/Astrobiology, ESA-SP 496, 2001, pp. 183-186.
2. G. Horneck Exobiological experiments in Earth orbit Adv. Space Res. 22 1998 317-326
3. G. Horneck, D.D. Wynn-Williams, R.L. Mancinelli, J. Cadet, N. Munakata, G. Rontö, H.G.M. Edwards, B. Hock, H. Wänke, G. Reitz, T. Dachev, D.P. Häder, C. Brioullet, Biological experiments on the Expose facility of the International Space Station, in: Proceedings of the 2nd European Symposium on the Utilization of the International Space Station, ESA SP-433, 1999, pp. 459-468.
4. G.M. Munoz Caro U.J. Meierhenrich W.A. Schutte B. Barbier A. Arcones Segovia H. Rosenbauer W.H. Thiemann A. Brack J.M. Greenberg Amino acids from ultraviolet irradiation of interstellar ice analogues Nature 416 2002 403-406
5. F. Chyba P.J. Thomas L. Brookshaw C. Sagan Cometary delivery of organic molecules to the early Earth Science 249 1990 366-373
6. P. Weber J.M. Greenberg Can spores survive in interstellar space? Nature 316 1985 403-407
7. G. Horneck Responses of Bacillus subtilis spores to the space environment: Results from experiments in space Orig. Life Evol. Biosph. 23 1993 37-52
8. W.L. Nicholson N. Munakata G. Horneck H.J. Melosh P. Setlow Resistance of Bacillus endospores to extreme terrestrial and extraterrestrial environments Microbiol. Mol. Biol. 64 2000 548-572
9. G.F. Joyce The antiquity of RNA-based evolution Nature 418 2002 214-221
10. F. Scappini F. Casadei R. Zamboni M. Franchi E. Gallori S. Monti Protective effect of clay minerals on adsorbed nucleic acid against UV radiation: Possible role in the origin of life Int. J. Astrobiol. 3 2004 17-19
11. A. Ciaravella F. Scappini M. Franchi C. Cecchi-Pestellini M. Barbera R. Candia E. Gallori G. Micela Role of clays in protecting adsorbed DNA against X-ray radiation Int. J. Astrobiol. 3 2004 31-35
12. S. Gáspár K. Módos Gy. Rontó Complex method for the determination of the physiological parameters of bacterium-phage systems L. Fedina Advances in Physiological Science 31 1981 Pergamon Oxford 141-147
13. A. Fekete A.A. Vink S. Gáspár K. Módos A. Bérces Gy. Rontó L. Roza Assessment of the effects of various UV sources on inactivation and photoproduct induction in phage T7 dosimeter Photochem. Photobiol. 68 1998 527-533
14. A. Fekete I. Földvári M. Hegedüs K. Módos G. Rontó Gy. Kovács A. Bérces Á. Péter Study of the effect of simulated space environment on phage T7 and isolated T7 DNA thin films J. Lumin. 102-103 2003 469-475
15. M. Falk K.A. Hartman R.C. Lord Hydration of deoxyribonucleic acid. I. A gravimetric study J. Am. Chem. Soc. 84 1962 3843-3846
16. Gy. Rontó A. Bérces A. Fekete G. Kovács P. Gróf H. Lammer Biological UV dosimeters in simulated space conditions Adv. Space Res. 33 2004 1302-1305
17. Gy. Rontó K. Sarkadi I. Tarján Zur analyse der UV dosiswirkungskurven der T7 phagen Strahlentherapie 134 1967 151-157
18. A. Fekete Gy. Rontó L.A. Feigin V.V. Tikchonychev K. Módos Temperature dependent structural changes of intraphage DNA Biophys. Struct. Mech. 9 1982 1-9
19. J. Sambrook E.F. Fritsch T. Maniatis Molecular Cloning: A Laboratory Manual 1989 Cold Spring Harbor Laboratory Press NY, U.S.A.
20. S.E. Freeman A.D. Blackett D.C. Monteleone R.B. Setlow B.M. Sutherland J.C. Sutherland Quantitation of radiation-, chemical-, or enzyme induced single strand breaks in nonradiactive DNA by alkaline agarose gel electrophoresis: Application to pyrimidine dimers Anal. Biochem. 158 1986 119-129
21. A. Zhitkovich M. Costa A simple, sensitive assay to detect DNA-protein cross-links in intact cells and in vivo Carcinogenesis 13 1992 1485-1489
22. M. Hegedüs K. Módos Gy. Rontó A. Fekete Validation of phage T7 biological dosimeter by quantitative polymerase chain reaction using short and long segments of phage T7 DNA Photochem. Photobiol. 78 2003 213-219
23. G. Horneck European activities in exobiology: Results and perspective Adv. Space Res. 23 1999 381-386
24. A. Fekete Gy. Rontó M. Hegedüs K. Módos A. Bérces G. Kovács H. Lammer C. Panitz Simulation experiments of the effect of space environment on bacteriophage and DNA thin films Adv. Space Res. 33 2004 1306-1310
25. Gy. Rontó M.M. Agamalyan G.M. Drabkin L.A. Feigin Y.M. Lvov Structure of bacteriophage T7: Small-angle X-ray and neutron scattering study Biophys. J. 43 1983 309-314
26. M.T. Milano W.A. Bernhard The effect of packing and conformation on free radical yields in films of variably hydrated DNA Radiat. Res. 151 1999 39-49
27. Z. Shakked G. Guerstein-Guzikevich M. Eisenstein F. Frolow D. Rabinovich The conformation of the DNA double helix in the crystal is dependent on its environment Nature 342 1989 456-460
28. D.S. Goodsell Inside a living cell Trends Biochem. Sci. 16 1991 203-206
29. W.C. Earnshaw S.R. Casjens DNA packaging by the double stranded DNA bacteriophages Cell 21 1980 319-331
30. S.G. Swarts M.D. Sevilla D. Becker C.J. Tokar K.T. Wheeler Radiation-induced DNA damage as a function of hydration Radiat. Res. 129 1992 333-344
31. A. Fekete, K. Módos, M. Hegedüs, G. Kovács, Gy. Rontó Á. Péter, H. Lammer, C. Panitz, DNA damage under simulated extraterrestrial conditions in bacteriophage T7, Adv. Space Res., in press.
32. C.S. Cox Roles of water molecules in bacteria and viruses Origins Life Evolut. Biosphere 23 1993 29-36
33. G. Horneck H. Bucker K. Dose K.D. Martens A. Bieger H.D. Menningmann G. Reitz H. Requardt P. Weber Microorganisms and biomolecules in space environment experiments ES 029 on Spacelab-1 Adv. Space Res. 4 1984 19-27
34. K. Dose A. Bieger-Dose R. Dillmann UV photobiochemistry under space conditions Adv. Space Res. 12 1996 51-60
35. F. Abad C. Villena S. Guix S. Caballero R.M. Pinto A. Bosch Potential role of fomites in the vehicular transmission of human astroviruses Appl. Environ. Microbiol. 67 2001 3904-3907
36. J. Cadet C. Anselmino T. Douki L. Voituriez New trends in photobiology: Photochemistry of nucleic acids in cells J. Photochem. Photobiol. B: Biol. 15 1992 277-298
37. G. Horneck A. Brack Study of the origin, evolution and distribution of life with emphasis on exobiology experiments in earth orbit Adv. Space Biol. Med. 2 1992 229-262
38. A.J. Varghese 5-thyminyl-5,6-dihydfrom from DNA irradiated with ultraviolet light Biochem. Biophys. Res. Commun. 38 1970 484-490
39. A. Fekete A.A. Vink S. Gáspár K. Módos A. Bérces Gy. Rontó L. Roza Influence of phage proteins on formation of specific UV DNA photoproducts in phage T7 Photochem. Photobiol. 69 1999 545-552
40. T. Douki G. Laporte J. Cadet Inter-strand photoproducts are produced in high yield within A-DNA exposed to UV-C radiation Nucleic Acids Res. 31 2003 3134-3142
41. G.P. Pfeifer Formation and processing of UV photoproducts: Effects of DNA sequence and chromatin environment Photochem. Photobiol. 65 1997 270-283
42. M. Folkard K. Prise B. Vojnovic S. Davies M.J. Roper B.D. Michael Measurements of DNA damage by electrons with energies between 25 and 4000 eV Int. J. Radiat. Biol. 64 1993 651-658
43. T. Douki J. Cadet Formation of the spore photoproduct and other dimeric lesions between adjacent pyrimidines in UVC-irradiated dry DNA Photochem. Photobiol. Sci. 4 2003 433-436
44. T. Douki B. Setlow P. Setlow Effects of the binding of alpha/beta-type small, acid-soluble spore proteins on the photochemistry of DNA in spores of Bacillus subtilis and in vitro Photochem. Photobiol. 81 2005 163-169
45. K. Dose A. Bieger-Dose R. Dillman M. Gill O. Kerz A. Klein H. Meinert T. Nawroth S. Risi C. Stridde ERA-Experiment "Space Biochemistry" Adv. Space Res. 16 1995 119-129
46. G.J. Fisher H.E. Johns Pyrimidine photodimers S.Y. Wang Photochemistry and Photobiology of Nucleic Acids vol. 1 1976 Academic Press New York 226-289