Direct identification of mycobacteria in primary liquid detection media by partial sequencing of the 65-kilodalton heat shock protein gene

Journal of Clinical Microbiology

Volumn 44, Issue 1, 2006, Pages 60-66

  McNabb, Alan ^a   Adie, Kathy ^a   Rodrigues, Mabel ^a   Black, William A ^a,b   Isaac Renton, Judith ^a,b  

^a BRITISH COLUMBIA CENTRE FOR DISEASE CONTROL   (Canada)

^b UNIVERSITY OF BRITISH COLUMBIA   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

GENE PRODUCT; HEAT SHOCK PROTEIN 65; RNA 16S;

ACID FAST BACTERIUM; ALGORITHM; ARTICLE; BACTERIUM IDENTIFICATION; BACTERIUM ISOLATE; COST CONTROL; CULTURE MEDIUM; FEASIBILITY STUDY; FLUORESCENCE; GENE; GENE SEQUENCE; HSP65 GENE; LABORATORY; MYCOBACTERIUM; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; RNA SEQUENCE; SMEAR;

BACTERIAL PROTEINS; BACTERIAL TYPING TECHNIQUES; CHAPERONINS; CULTURE MEDIA; DNA, BACTERIAL; MYCOBACTERIUM; RNA, RIBOSOMAL, 16S; SEQUENCE ANALYSIS, DNA;

BACTERIA (MICROORGANISMS); CORYNEBACTERINEAE; MYCOBACTERIUM;

EID: 30744441673     PISSN: 00951137     EISSN: None     Source Type: Journal    
DOI: 10.1128/JCM.44.1.60-66.2006     Document Type: Article

References (23)

1. Adekambi, T., M. Reynaud-Gaubert, G. Greub, M. J. Gevaudan, B. La Scola, D. Raoult, and M. Drancourt. 2004. Amoebal coculture of "Mycobacterium massilense" sp. nov. from the sputum of a patient with hemoptoic pneumonia. J. Clin. Microbiol. 42:5493-5501.
2. Applied Biosystems Inc. 2001. Users manual, AB1 Prism 3100 genetic analyzer. Applied Biosystems Inc., Foster City, Calif.
3. Beggs, M. L., R. Stevanova, and K. D. Eisenach. 2000. Species identification of Mycobacterium avium complex isolates by a variety of molecular techniques. J. Clin. Microbiol. 38:508-512.
4. Brunello, F., M. Ligozzi, E. Cristelli, S. Bonora, E. Tortoli, and R. Fontana. 2001. Identification of 54 mycobacterial species by PCR-restriction fragment length polymorphism analysis of the hsp65 gene. J. Clin. Microbiol. 39:2799-2806.
5. Da Silva Rocha, A., C. da Costa Leite, H. Magarinos Torres, A. B. de Miranda, M. Q. Pires Lopes, W. M. Degrave, and P. N. Suffys. 1999. Use of PCR-restriction fragment length polymorphism analysis of the hsp65 gene for rapid identification of mycobacteria in Brazil. J. Microbiol. Methods 37:223-229.
6. de Magalhaes, V. D., P. de Melo Azevedo Fda, J. Pasternak, and M. D. Valle Martino. 2002. Reliability of hsp65-RFLP analysis for identification of Mycobacterium species in cultured strains and clinical specimens. J. Microbiol. Methods 49:295-300.
7. Devallois, A., K. S. Goh, and N. Rastogi. 1997. Rapid identification of mycobacteria to species level by PCR-restriction fragment length polymorphism analysis of the hsp65 gene and proposition of an algorithm to differentiate 34 mycobacterial species. J. Clin. Microbiol. 35:2969-2973.
8. Ergin, A., T. Kocagoz, and D. Us. 2000. Evaluation of 120 mycobacterial strains isolated from clinical specimens to the species level by polymerase chain reaction-restriction enzyme analysis. Scand. J. Infect. Dis. 32:657-662.
9. Hernandez, M. S., G. P. Morlock, W. R. Butler, J. T. Crawford, and R. C. Cooksey. 1999. Identification of Mycobacterium species by PCR-restriction fragment length polymorphism analyses using fluorescence capillary electrophoresis. J. Clin. Microbiol. 37:3688-3692.
10. Hosojima, A. C. S., Y. Fukasawa, Y. Kazumi, M. Takahashi, K. Hirano, and T. Mori. 1992. Comparison of MB-Check, Bactec, and egg-based media for recovery of mycobacteria. J. Clin. Microbiol. 30:878-881.
11. Levi, M. H., J. Bartell, L. Gandolfo, S. C. Smole, S. F. Costa, L. M. Weiss, L. K. Johnson, G. Osterhout, and L. H. Herbst. 2003. Characterization of Mycobacterium montefiorense sp. nov., a novel pathogenic mycobacterium from moray eels that is related to Mycobacterium triplex. J. Clin. Microbiol. 41:2147-2152.
12. McNabb, A., D. Eisler, K. Adie, M. Amos, M. Rodrigues, G. Stephens, W. A. Black, and J. Isaac-Renton. 2004. Assessment of partial sequencing of the 65-kilodalton heat shock protein gene (hsp65) for routine identification of Mycobacterium species isolated from clinical sources. J. Clin. Microbiol. 42:3000-3011.
13. Padilla, E., V. Gonzalez, J. M. Manterola, A. Perez, M. D. Quesada, S. Gordillo, C. Vilaplana, M. A. Pallares, S. Molinos, M. D. Sanchez, and V. Ausina. 2004. Comparative evaluation of the new version of the Inno-Lipa mycobacteria and genotype mycobacterium assays for identification of Mycobacterium species from MB/BacT liquid cultures artificially inoculated with mycobacterial strains. J. Clin. Microbiol. 42:3083-3088.
14. Pai, S., N. Esen, X. Pan, and J. M. Musser. 1997. Routine rapid Mycobacterium species assignment based on species-specific allelic variation in the 65-kilodalton heat shock protein gene (hsp65). Arch. Pathol. Lab. Med. 121:859-864.
15. Patel, J. B., R. J. Wallace, Jr., B. A. Brown-Elliott, T. Taylor, C. Imperatrice, D. G. B. Leonard, R. W. Wilson, L. Mann, K. C. Jost, and I. Nachamkin. 2004. Sequence-based identification of aerobic actinomycetes. J. Clin. Microbiol. 42:2530-2540.
16. Ringuet, H., C. Akoua-Koffi, S. Honore, A. Varnerot, V. Vincent, P. Berche, J. L. Gaillard, and C. Pierre-Audigier. 1999. Hsp65 sequencing for identification of rapidly growing mycobacteria. J. Clin. Microbiol. 37:852-857.
17. Sanguinetti, M., B. Posteraro, F. Ardito, S. Zanetti, A. Cingolani, L. Sechi, A. De Luca, L. Ortona, and G. Fadda. 1998. Routine use of PCR-reverse cross-blot hybridization assay for rapid identification of Mycobacterium species growing in liquid media. J. Clin. Microbiol. 36:1530-1533.
18. Steingrube, V. A., J. L. Gibson, B. A. Brown, Y. Zhang, R. W. Wilson, M. Rajagonpalan, and R. J. Wallace, Jr. 1995. PCR amplification and restriction endonuclease analysis of a 65-kilodalton heat shock protein gene sequence for taxonomic separation of rapidly growing mycobacteria. J. Clin. Microbiol. 33:149-153.
19. Taylor, T. B., C. Patterson, Y. Hale, and W. W. Safranek. 1997. Routine use of PCR-restriction fragment length polymorphism analysis for identification of mycobacteria growing in liquid media. J. Clin. Microbiol. 35:79-85.
20. Tortoli, E., L. Rindi, M. J. Garcia, P. Chiaradonna, R. Dei, C. Garzelli, M. Kroppenstedt, N. Lari, R. Mattei, A. Mariottini, G. Mazzarelli, M. I. Murcia, A. Nanetti, P. Piccoli, and C. Scarparo. 2004. Proposal to elevate the genetic variant MAC-A, included in the Mycobacterium avium complex, to species rank as Mycobacterium chimaera sp. nov. Int. J. Syst. Evol. Microbiol. 54:1277-1285.
21. Vincent, V., B. A. Brown-Elliott, K. C. Jost, Jr., and R. J. Wallace, Jr. 2003. Mycobacterium: phenotypic and genotypic identification, p. 560-584. In P. R. Murray, E. J. Baron, J. H. Jorgensen, M. A. Pfaller, and R. H. Yolken (ed.), Manual of clinical microbiology, vol. 1, 8th ed. ASM Press, Washington, D.C.
22. Woo, P. C. Y., A. H. Y. Ngan, S. K. P. Lau, and K. Yuen. 2003. Tsukamurella conjunctivitis: a novel clinical syndrome. J. Clin. Microbiol. 41:3368-3371.
23. Zeller, V., A. Nardi, C. Truffot-Pernot, W. Sougakoff, B. Stankoff, C. Katlama, and F. Bricaire. 2003. Disseminated infection with a Mycobacterium related to Mycobacterium triplex with central nervous system involvement associated with AIDS. J. Clin. Microbiol. 41:2785-2787.