A rapid alternative technique for obtaining silver-positive patterns in chromosomes

Genetics and Molecular Biology

Volumn 27, Issue 2, 2004, Pages 196-198

  Kavalco, Karine Frehner ^a   Pazza, Rubens ^a  

^a FEDERAL UNIVERSITY OF SÃO CARLOS   (Brazil)

Author keywords

Ag NOR; Chromosome banding; Microwaves; Silver staining

Indexed keywords

REAGENT;

ANIMAL CELL; ARTICLE; CHROMOSOME ANALYSIS; CHROMOSOME NOR; CONTROLLED STUDY; INTERMETHOD COMPARISON; MICROWAVE RADIATION; NONHUMAN; PRECIPITATION; QUALITY CONTROL; RELIABILITY; SILVER STAINING; TIME MANAGEMENT;

ANIMALIA;

EID: 30644459541     PISSN: 14154757     EISSN: 16784685     Source Type: Journal    
DOI: 10.1590/S1415-47572004000200012     Document Type: Article

References (21)

1. Bertollo LAC, Takahashi CS and Moreira-Filho O (1978) Cytotaxonomic considerations on Hoplias lacerdae (Pisces, Erythrinidae). Rev Bras Genet 1(2):103-120.
2. Clavaguera A, Querol E, Dolors C, Genesca J and Egozcue J (1983) Cytochemical studies on the nature of NOR (nucleolus organizer region) silver stainability. Cell Mol Biol 29:255-259.
3. Dobigny G, Ozouf-Costaz C, Bonillo C and Volobouev V (2002) "Ag-NORs" are not always true NORs: new evidence in mammals. Cytogenet Genome Res 98:75-77.
4. Galetti Jr PM (1998) Chromosome diversity in Neotropical fishes: NOR studies. Ital J Zool 65:53-56.
5. Goessens G (1984) Nucleolar structure. Int Rev Cytol 84:107-158.
6. Goodpasture C and Bloom SE (1975) Visualization of nucleolar organizer regions in mammalian chromosomes using silver staining. Chromosoma 53:37-50.
7. Hernández-Verdún D (1986) Structural organization of the nucleolus in mammalian cells. Meth Archiv Exp Pathol 12:26-62.
8. Howell WM (1977) Visualization of ribosomal gene activity: silver stain proteins associated with RNA transcribed from oocyte chromosomes. Chromosoma 62:361-367.
9. Howell WM and Black DA (1980) Controlled silver-staining of nucleolus organizer regions with a protective colloidal developer: a 1-step method. Experientia 36:1014-1015.
10. Hubbel HR (1985) Silver staining as an indicator of active ribosomal genes. Stain Technol 60:285-294.
11. Jiménez R, Burgos M and Diaz de la Guardia R (1988) A study of the silver staining significance in mitotic NORs. Heredity 60:125-127.
12. Li Q, Hacker GW, Danscher G, Sonnleitner-Wittauer U and Grimelius L (1995). Argyrophilic nucleolar organizer regions. A revised version of the Ag-NOR-staining technique. Histochem Cell Biol 104(2):145-150.
13. Medina FJ, Risueño MC, Sánchez-Pina MA and Fernandez-Gomez ME (1983) A study on nucleolar silver staining in plant cells. The role of argyrophilic proteins in nucleolar physiology. Chromosoma 88:149-155.
14. Medina FJ, Cerdido A and Marco R (1995) Microwave irradiation improvements in the silver staining of the nucleolar organizer (Ag-NOR) technique. Histochem. Cell Biol 103(6):403-413.
15. Miller DA, Dev VG, Tantravahi R and Miller OJ (1976) Suppression of human nucleolus organizer in mouse-human somatic hybrid cells. Exp Cell Res 101:235-243.
16. Pich A, Chiusa L and Margaria E (2000) Prognostic relevance of AgRONs in tumor pathology. Micron 31:133-141.
17. Pinkel D, Straume T and Gray JW (1986) Cytogenetic analysis using quantitative, high-sensitivity, fluorescence hybridization. Proc Natl Acad Sci 83:2934-2938.
18. Rufas JS, Iturra P, De Souza W and Esponda P (1982) Simple silver staining procedure for the localization of nucleolus and nucleolar organizers under light and electron microscopy. Arch Biol 93:267-276.
19. Sanchez A, Jiménez R, Burgos M, Stitou S, Zurita F and Diaz de la Guardia R (1995) Cytogenetic peculiarities in the Algerian hedgehog: silver stains not only NORs but also heterochromatic blocks. Heredity 75:10-16.
20. Sánchez-Pina MA, Medina FJ, Fernández-Gómez ME and Risueño MC (1984) Ag-NOR proteins are present when transcription is impaired. Biol Cell 5:199-202.
21. Sumner AT (1990) Chromosome Banding. Unwin Hyman, London.