Expression of tobacco cDNA encoding phytochelatin synthase promotes tolerance to and accumulation of Cd and As in Saccharomyces cerevisiae

Journal of Plant Biology

Volumn 48, Issue 4, 2005, Pages 440-447

  Young, Jin Kim ^a   Kwang, Seok Chang ^a   Mi, Ran Lee ^a   Jong, Hoon Kim ^a   Chang, Eun Lee ^a   Yoon, Joo Jeon ^a   Jong, Soon Choi ^b   Hyoung, Sun Shin ^b   Hwang, Seongbin ^a  

^a Sejong University   (South Korea)

^b Korea Basic Science Institute (KBSI)   (South Korea)

Author keywords

Accumulation; Arsenite; Cadmium; Nicotiana tabacum phytochelatin synthase; Saccharomyces cerevisiae; Tolerance

Indexed keywords

NICOTIANA TABACUM; SACCHAROMYCES CEREVISIAE;

EID: 30444451163     PISSN: 12269239     EISSN: None     Source Type: Journal    
DOI: 10.1007/BF03030586     Document Type: Article

References (25)

1. Cazale AC, Clemens S (2001) Arabidopsis thaliana expresses a second functional phytochelatin synthase. FEBS Lett 507: 215-219
2. Chen J, Zhou J, Goldsbrough PB (1997) Characterization of phytochelatin synthase from tomato. Physiol Plant 101: 165-172
3. Clemens S, Kim EJ, Neumann D, Schroeder JI (1999) Tolerance to toxic metals by a gene family of phytochelatin synthases from plants and yeast. EMBO J 18: 3325-3333
4. Cullen WR, Reimer KJ (1989) Arsenic speciation in the environment. Chem Rev 89: 713-764
5. Gietz RD, Schiestl RH (1995) Transforming yeast with DNA. Methods Mol Cell Biol 5: 255-269
6. Grill E, Winnacker EL, Zenk MH (1987) Phytochelatins, a class of heavy-metal binding peptides from plants, are functionally analogous to metallothioneins. Proc Natl Acad Sci USA 84: 439-443
7. Ha SB, Smith AP, Howden R, Dietrich WM, Bugg S, O'Connell MJ, Goldsbrough PB, Cobbett CS (1999) Phytochelatin synthase genes from Arabidopsis and the yeast Schizosaccharomyces pombe. Plant Cell 11: 1153-1164
8. Howden R, Goldsbrough PB, Anderson CR, Cobbett CS (1995) Cadmium-sensitive, cad1, mutants of Arabidopsis thaliana are phytochelatin deficient. Plant Physiol 107: 1059-1066
9. Li ZS, Lu YP, Zhen RG, Szczypka M, Thiele DJ, Rea PA (1997) A new pathway for vacuolar cadmium sequestration in Saccharomyces cerevisiae: YCF-1 catalyzed transport of bis(glutathionate)cadmium. Proc Natl Acad Sci USA 94: 42-47
10. Li ZS, Szczypka M, Lu YP, Thiele DJ, Rea PA (1996) The yeast cadmium factor protein (YCF1) is a vacuolar glutathione S-conjugate pump. J Biol Chem 271: 6509-6517
11. Lu YP, Li ZS, Rea PA (1997) AtMRP1 gene of Arabidopsis encodes a glutathione S conjugate pump: Isolation and functional definition of a plant ATP-binding cassette transporter gene. Proc Natl Acad Sci USA 94: 8243-8248
12. Maitani T, Kuboti H, Sato K, Yamada T (1996) The composition of metal bound to class III metallothionein (phytochelatin and its desglycyl peptide) induced by various metals in root cultures of Rubia tinctorum. Plant Physiol 110: 1145-1150
13. Ortiz DF, Kreppel L, Speiser DM, Scheel G, McDonald G, Ow DW (1992) Heavy metal tolerance in the fission yeast requires an ATP-binding cassette-type vacuolar membrane transporter. EMBO J 11: 3491-3499
14. Oven M, Page JE, Zenk MH, Kutchan TM (2002) Molecular characterization of the homo phytochelatin synthase of soybean Glycine max: Relation to phytochelatin synthase. J Biol Chem 277: 4747-4754
15. Sauge-Merle S, Cuine S, Carrier P, Lecomte-Pradines C, Luu DT, Peltier G (2003) Enhanced toxic metal accumulation in engineered bacterial cells expressing Arabidopsis thaliana phytochelatin synthase. Appl Environ Microbiol 69: 490-494
16. Schmöger MEV, Oven M, Grill E (2000) Detoxification of arsenic by phytochelatins in plants. Plant Physiol 122: 793-801
17. Scott OR, Arnold JB (1988) Extraction of DNA from plant tissues, In Plant Molecular Biology Manual A6. Kluwer Academic Publishers, Dordrecht, pp 1-11
18. Silver S (1996) Bacterial resistance to toxic metal ions: A review. Gene 179: 9-19
19. Silver S, Phung LT (1996) Bacterial heavy metal resistance: New surprises. Annu Rev Microbiol 50: 753-789
20. Thompson JD, Higgins DG, Gibson TJ (1994) CLUSTAL W: Improving the sensitivity of progressive multiple sequence alignment through sequence weighting, positions-specific gap penalties and weight matrix choice. Nucleic Acids Res 22: 4673-4680
21. Vatamaniuk OK, Mari S, Lu YP, Rea PA (1999) AtPCS1, a phytochelatin synthase from Arabidopsis: Isolation and in vitro reconstitution. Proc Natl Acad Sci USA 96: 7110-7115
22. Vogeli-Lange R, Wagner GJ, Dziadkowiec D (1990) Subcellular localization of cadmium and cadmium-binding peptides in tobacco leaves: Implication of a transport function for cadmium-binding peptides. Plant Physiol 109: 945-954
23. Wysocki R, Bobrowicz P, Ulaszewski S (1997) The Saccharomyces cerevisiae ACR3 gene encodes a putative membrane protein involved in arsenite transport. J Biol Chem 272: 30061-30066
24. Wysocki R, Clemens S, Augustyniak D, Golik P, Maciaszczyk E, Tamas MJ (2003) Metalloid tolerance based on phytochelatins is not functionally equivalent to the arsenite transporter Acr3p. Biochem Biophys Res Commun 304: 293-300
25. Zenk MH (1996) Heavy metal detoxification in higher plants: A review. Gene 179: 21-30