Preincubation of rat and human hepatocytes with cytoprotectants prior to cryopreservation can improve viability and function upon thawing

Liver Transplantation

Volumn 11, Issue 12, 2005, Pages 1533-1540

  Terry, Claire ^a   Dhawan, Anil ^a   Mitry, Ragai R ^a   Lehec, Sharon C ^a   Hughes, Robin D ^a  

^a KING'S COLLEGE LONDON   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

ACETYLCYSTEINE; ADENOSINE TRIPHOSPHATE; ALBUMIN; ANTIOXIDANT; ASCORBIC ACID; FRUCTOSE; GLUCOSE; GLUTATHIONE; LACTATE DEHYDROGENASE; PENTOXIFYLLINE; PROSTAGLANDIN E1; S ADENOSYLMETHIONINE; TAUROURSODEOXYCHOLIC ACID; THIOCTIC ACID;

ANIMAL CELL; ANIMAL TISSUE; ARTICLE; CELL FUNCTION; CELL ISOLATION; CELL PROTECTION; CELL VIABILITY; CONCENTRATION RESPONSE; CONTROLLED STUDY; CRYOPRESERVATION; FREEZING; HEPATOCYTE TRANSPLANTATION; HUMAN; HUMAN CELL; HUMAN TISSUE; IN VITRO STUDY; INCUBATION TIME; LIVER DISEASE; MALE; NONHUMAN; PRIORITY JOURNAL; RAT; THAWING;

ANIMALS; ANTIOXIDANTS; CELL SURVIVAL; CELLS, CULTURED; CRYOPRESERVATION; CYTOPROTECTION; HEPATOCYTES; HUMANS; LIVER TRANSPLANTATION; MALE; ORGAN PRESERVATION; RATS; RATS, SPRAGUE-DAWLEY;

EID: 30444437207     PISSN: 15276465     EISSN: None     Source Type: Journal    
DOI: 10.1002/lt.20503     Document Type: Article

References (30)

1. Rijintes PJM, Moshage HJ, Van Gemert PJL, De Waal R, Yap, SH. Cryopreservation of adult human hepatocytes: the influence of deep freezing storage on the viability, cell seeding, survival, fine structures and albumin synthesis in primary cultures. J Hepatol 1986;3:7-18.
2. Loretz LJ, Li AL, Flye MW, Wilson AGE. Optimization of cryopreservation procedures for rat and human hepatocytes. Xenobiotica 1989;19:489-498.
3. Dou M, De Sousa G, Lacarelle B, Placidi M, De La Porte PL, Domingo M, et al. Thawed human hepatocytes in primary culture. Cryobiology 1992;29:454-469.
4. Diener B, Traiser M, Arand M, Leissner J, Wirsch U, Hohenfellner R, et al. Xenobiotic metabolising enzyme activities in isolated and cryopreserved human liver parenchymal cells. Toxicol In Vitro 1994;8:1161-1166.
5. Adams RM, Wang M, Crane AM, Brown B, Darlington GJ, Ledley FD. Effective cryopreservation and long-term storage of primary human hepatocytes with recovery of viability, differentiation, and replicative potential. Cell Transplant 1995;4:570-586.
6. Li AP, Lu C, Brent JA, Pham C, Fackett A, Ruegg CE, Silber PM. Cryopreserved human hepatocytes: characterization of drug-metabolising enzyme activities and applications in higher throughput screening assays for hepatotoxicity, metabolic stability and drug-drug interaction potential. Chem Biol Interact 1999;121:17-35.
7. Steinberg P, Fischer T, Kiulies S, Biefang K, Platt KL, Oesch F, et al. Drug metabolizing capacity of cryopreserved human, rat, and mouse liver parenchymal cells in suspension. Drug Metab Disp 1999;27:1415-1422.
8. Hengstler JG, Utesch D, Steinberg P, Platt KL, Diener B, Ringel M, et al. Cryopreserved primary hepatocytes as a constantly available in vitro model for the evaluation of human and animals drug metabolism and enzyme induction. Drug Metab Rev 2000; 32:81-118.
9. Ostrowska A, Bode DC, Pruss J, Bilir B, Smith GD, Zeisloft S. Investigation of functional and morphological integrity of freshly isolated and cryopreserved human hepatocytes. Cell Tissue Bank 2000;1:55-68.
10. Alexandre E, Viollon-Abadie C, David P, Gandillet A, Coassolo P, Heyd B, et al. Cryopreservation of adult human hepatocytes obtained from rejected liver biopsies. Cryobiology 2002;44:103-113.
11. Li AP, Gorycki PD, Hengstler JG, Kedderis GL, Koebe HG, Rahmani R, et al. Present status of the application of cryopreserved hepatocytes in the evaluation of xenobiotics: consensus of an international expert panel. Chem Biol Interact 1999;121:117-123.
12. Zaleski J, Richburg J, Kauffman FC. Preservation of the rate and profile of xenobiotic metabolism in rat hepatocytes stored in liquid nitrogen. Biochem Pharm 1993;46:111-116.
13. Silva JM, Day SH, Nicoll-Griffith D. Induction of cytochrome P450 in cryopreserved rat and human hepatocytes. Chem Biol Interact 1999;121:49-63.
14. Reese JA, Byard JL. Isolation and culture of adult hepatocytes from liver biopsies. In Vitro 1981;17:935-940.
15. Strom SC, Dorko K, Thompson MT, Pisarov LA, Nussler AK. Large scale isolation and culture of human hepatocytes. In: Franco D, Boudjema K, Varet B, eds. Ilots de Langerhans et Hepatocytes. Paris: Les Editions INSERM, 1982. 195-205.
16. Mitry RR, Hughes RD, Aw MM, Terry C, Mieli-Vergani G, Girlanda R, et al. Human hepatocyte isolation and relationship of cell viability to early graft function. Cell Transplant 2003;12: 69-74.
17. Diener B, Utesch D, Beer N, Durk H, Oesch F. A method for the cryopreservation of liver parenchymal cells for studies of xenobiotics. Cryobiology 1993;30:116-127.
18. Lowry OH, Roseburgh NJ, Farr AL, Randall RJ. Protein measurement with the Folin phenol reagent. J Biol Chem 1951;193: 265-275.
19. Donato MT, Gomez-Lechon MJ, Castell JV. A microassay for measuring cytochrome P450IA1 and P450IIB1 activities in intact human and rat hepatocytes cultured on 96-well plates. Anal Biochem 1993;213:29-33.
20. Schilling M, Tian YH, Buchler MW. Effect of temperature on hepatic and renal uptake of components from University of Wisconsin solution. Transplantation 1998;65:989-992.
21. Ladriere L, Mercan D, Bjorkling F, Malaisse WJ. Improved viability and metabolic behaviour of hepatocytes after liver storage in the presence of a succinic acid ester. Transplantation 1998;66:183-185.
22. Frenzel J, Richter J, Eschrich K. Fructose inhibits apoptosis induced by reoxygenation in rat hepatocytes by decreasing reactive oxygen species via stabilization of the glutathione pool. Biochim Biophys Acta 2002;1542:82-94.
23. Hochachka PW, Buck LT, Doll CJ, Land SC. Unifying theory of hypoxia tolerance: molecular/metabolic defense and rescue mechanisms for surviving oxygen lack. Proc Natl Acad Sci USA 1996;93:9493-9498.
24. Ulrich RG, Cramer CT, Sun EL, Bacon JA, Petrella DK. A protocol for isolation, culture, and cryopreservation of hepatocytes from human liver. In Vitr Mol Toxicol 1998;11:23-33.
25. Baccarani U, Sanna A, Cariani A, Sainz-Barriga M, Adani GL, Zambito AM, et al. Isolation of human hepatocytes from livers rejected for liver transplantation on a national basis: results of a 2-year experience. Liver Transpl 2003;9:506-512.
26. Evdokimova E, Martos M, Buc Calderon PM. Protective effect of fructose on survival and metabolic capacities of hepatocytes kept overnight under cold hypoxia before normothermic reoxygenation. Food Chem Toxicol 1997;35:669-675.
27. Bustamante J, Lodge JK, Marcocci L, Tritschler HJ, Packer L, Rihn BH. Alpha-lipoic acid in liver metabolism and disease. Free Radic Biol Med 1998;24:1023-1039.
28. Han D, Handelman G, Marcocci L, Sen CK, Roy S, Kobuchi H, et al. Lipoic acid increases de novo synthesis of cellular glutathione by improving cysteine utilization. Biofactors 1997;6:321-338.
29. Hultberg B, Andersson A, Isaksson A. Lipoic acid increases glutathione production and enhances the effect of mercury in human cell lines. Toxicology 2002;175:103-110.
30. Muller C, Dunschede F, Koch E, Vollmar AM, Kiemer AK. Alpha-lipoic acid preconditioning reduces ischemia/reperfusion injury of the rat liver via the PI-3 kinase/AKT pathway. Am J Physiol Gastroenterol Liver Physiol 2003;285:G769-G778.