Establishment of a binding assay system for screening of the inhibitors of p56lck SH2 domain

Journal of Biochemistry and Molecular Biology

Volumn 31, Issue 4, 1998, Pages 370-376

  Kim, Jynho ^a   Hur, Eun Mi ^a   Yun, Yungdae ^a  

^a Mogam Biotechnology Research Institute   (South Korea)

Author keywords

Binding assay; Lck; Protein to protein interaction; Screening; SH2

Indexed keywords

EID: 3042885516     PISSN: 12258687     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (35)

1. Escobedo, J. A., Kaplan, D. R., Kavanaugh, W. M., Turck, C. W. and Williams, L. T. (1991) A phosphotidylinositol-3-kinase binds to platelet-derived growth factor receptors through a specific receptor sequence containing phosphotyrosine. Mol. Cell. Biol. 11, 1125-1132.
2. Bibbins, K. B., Boeuf, H. and Varmus, H. E. (1993) Binding of the Src SH2 domain to phosphopeptides is determined by residues in both the SH2 domain and the phosphopeptides. Mol. Cell. Biol. 13, 7278-7287.
3. Cantley, L. C., Auger, K. R., Carpenter, C., Duckworth, B., Graziaini, A., Kapeller, R. and Solotoff, S. (1991) Oncogenes and signal transduction. Cell 64, 281-302.
4. Coughlin, S. R., Escobedo, J. A. and Williams, L. T. (1989) Role of phosphatidylinositol kinase in PDGF receptor signal transduction. Science 243, 1191-1194.
5. Fantle, W. J., Escobrdo, J. A., Martin, G. A., Turck, C. W., delRosario, M., McCormick, F. and Williams, L. T. (1992) Distinct phosphotyrosines on a growth factor receptor bind to specific molecules that mediate different signaling pathways. Cell 69, 413-423.
6. Furka, A., Sebestyen, F., Asgedom, M. and Dibo, G. (1991) General method for rapid synthesis of multicomponent peptide mixtures. Int. J. Pept. Protein. Res. 37, 487-493.
7. Gilmer, T., Rodriguez, M., Jordan, S., Crosby, R., Allogood, K., Green, M., Kimery, M., Wagner, C., Kinder, D., Charifson, P., Hassell, A. M., Willard, D., Luther, M., Rusnak, D., Sternbach, D. D., Mehrotra, M., Peel, M., Shampine, L., Davis, R., Robbins, J., Patel, I. R., Kassel, D., Burkahrt, W., Moyer, M., Bradshaw, T. and Berman, J. (1994) Peptide inhibitors of src SH3-SH2-phosphoprotein interactions. J. Biol. Chem. 269, 31711-31719.
8. lck with CD4 in antigen-specific signal transduction in T cells. Cell 64, 511-520.
9. Jang, J. S., Kim, J. M., Chung, G. H., Paik, B. H. and Kim, H. J. (1996) A rapid and sensitive two-site sandwich enzyme-linked immunosorbent assay for detection of α-fetoprotein in Human serum. J. Biochem. Mol. Biol. (formerly Korean Biochem. J.) 29, 192-199.
10. lck regulates specificity of its Src homology 2 domain. Proc. Natl. Acad. Sci. USA 92, 5778-5782.
11. Kashishian, A., Kazlauskas, A. and Cooper, J. A. (1992) Phosphorylation sites in the PDGF receptor with different specificities for binding GAP and P13kinase in vivo. EMBO J 11, 1373-1382
12. Kazlauskas, A., Ellis, C., Pawson, T. and Cooper , J. A. (1990) Binding of GAP to activated PDGF receptors. Science 247, 1578-1581.
13. Kazlauskas, A., Kashishian, A., Cooper, A. and Valius, M. (1992) GTPase-activating protein and phosphatidylinositol-3-kinase bind to a distinct region of the platelet-derived growth factor receptor β subunit. Mol. Cell. Biol. 12, 2534-2544.
14. Kim, M.-H. (1997) Competitive enzyme-linked immunosorbent assay for glucose-6-phosphate dehydrogenase. J. Biochem. Mol. Biol. (formerly Korean Biochem. J.) 30, 326-331.
15. Koch, C. A. anderson, D., Moran, M. F., Ellis, C. and Pawson, T. (1991) SH2 and SH3 domains: elements that control interactions of cytoplasmic signaling proteins. Science 252, 668-674.
16. Koh, W. S., Yoon, S. Y., Kim, J. W., Lee, C. E. and Han, M. Y., (1997) A screening method for Src Homology 3 domain binding blockers based on Ras signaling pathway. J. Biochem. Mol. Biol. (formerly Korean Biochem. J.) 30, 303-307.
17. Kumjian, D. A., Wahl, M. I., Rhee, S. G. and Daniel, T. O. (1989) Platelet-derived growth factor (PDGF) binding promotes physical association of PDGF receptor with phospholipase C. Proc. Natl. Acad. Sci. USA 86, 8232-8236.
18. Ladbury, J. E., Lemmon, M. A., Zhou, M., Green, J., Botfield, M. C. and Schlessinger, J. (1995) Measurement of the binding of tyrosyl phosphopeptides to SH2 domains: A reappraisal. Proc. Natl. Acad. Sci. USA 92, 3199-3203.
19. lck. Nature 357, 161-164.
20. Molloy, C. J., Bottaro, D. P., Fleming, T. P., Marshall, M. S., Gibbs, J. B. and Aaronson, S. A. (1989) PDGF induction of tyrosine phosphorylation of GTPase activating protein. Nature 342, 711-714.
21. Pawson, T. (1995) Protein modules and signaling networks. Nature 373, 573-580.
22. Pawson, T. and Gish, G. D. (1992) SH2 and SH3 domains: from structure to function. Cell 71, 359-362.
23. src homology 2 domain binding to tyrosine-phosphorylated peptides determined by a competition assay or surface plasmon resonance. Proc. Natl. Acad. Sci. USA 90, 4902-4906.
24. Payne, G., Stolz, L. A., Pei, D., Band, H., Shoelson, S. E. and Walsh, C. T. (1994) The phosphopeptide-binding specificity of Src family SH2 domains. Chem. Bio. 1, 99-105.
25. lck with phosphotyrosine-containing proteins. Oncogene Res. 8, 2765-2772.
26. Perlmutter, R. M., Marth, J. D., Lewis, D. B., Peet, R., Ziegler, S. F. and Wilson, C. B. (1988) Structure and expression of lck transcripts in human lymphoid cells. J. Cell Biochem. 38, 117-126
27. Piccione, E., Case, R. D., Domchek, S. M., Hu, P., Chaudhuri, M., Backer, J. M., Schlessinger, J. and Shoelson, S. E. (1993) Phosphatidylinositol 3-kinase p85 SH2 domain specificity defined by direct phosphopeptide/SH2 domain binding. Biochemistry 32, 3197-3202.
28. Rotin, D., Margolis, B., Mohammadi, M., Daly, R. J., Dawn, G., Li, N., Fischer, E. H., Burgess, W. H., Ullrich, A. and Schlessinger, J. (1992) SH2 domains prevent tyrosine dephosphorylation of the EGF receptor: identification of Tyr992 as the high-affinity binding site for SH2 domains of phospholipase C gamma. EMBO J. 11, 559-567.
29. Songyang, Z., Shoelson, S. E., Chaudhuri, M., Gish, G., Pawson, T., Haser, W. G., King, F., Roberts, T., Ratnofsky, S., Lechleider, R. J., Neel, B. J., Birge, R. B., Fajardo, J. E., Chou, M. M., Hanafusa, H., Schaffhausen, B. and Cantley, L. C. (1993) SH2 domains recognize specific phosphopeptide sequences. Cell 72, 767-778.
30. Straus, D. B. and Weiss, A. (1992) Genetic evidence for the involvement of the lck tyrosine kinase in signal transduction through the T cell antigen receptor. Cell 70, 585-593.
31. lck with cytoplasmic domains of CD4 and CD8 is mediated by cysteine motifs. Cell 60, 755-765.
32. Vaskovsky, V. E., Kostetsky, E. Y. and Vasendin, I. M. (1975) A universal reagent for phospholipid analysis. J. Chromatogr. 114, 129-141.
33. lck by the non-catalytic SH2 and SH3 domains. Oncogene Res. 7, 971-980.
34. Wood, E. R., McDonald, O. B. and Sahyoun, N. (1992) Quantitative analysis of SH2 domain binding. Evidence for specificity and competition. J. Biol. Chem. 267, 14138-14144.
35. Zhu, G., Decker, S. J., Mayer, B. J. and Saltiel, A. R. (1993) Direct analysis of the binding of the abl Src homology 2 domain to the activated epidermal growth factor receptor. J. Biol. Chem. 268, 1775-1779.