Isolation, culture and characterization of a new strain of Euglena gracilis

Phycological Research

Volumn 52, Issue 2, 2004, Pages 168-173

  Ruiz, Laura B ^a   Rocchetta, Iara ^a   Dos Santos Ferreira, Verónica ^a   Conforti, Visitación ^a  

^a UNIVERSIDAD DE BUENOS AIRES   (Argentina)

Author keywords

Bleached; Culture; Euglena gracilis; Lipid; Protein; Strain; Sugar

Indexed keywords

FLAGELLATE; RIVER POLLUTION;

EUGLENA; EUGLENA GRACILIS; MASTIGOPHORA (FLAGELLATES);

EID: 3042827100     PISSN: 13220829     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1440-1835.2004.tb00325.x     Document Type: Article

References (25)

1. (APHA) American Public Health Association. 1975. Standard Methods for the Examination of Water and Wastewaters, 14th edn. APHA, AWWA, WPCF, Washington.
2. Barsanti, L., Vismara, R., Passarelli, V. and Gualtieri, P. 2000. Paramylon (β-1,3-glucan) content in wild type and WZSL mutant of Euglena gracilis: effects of growth conditions. J. Appl. Phycol. 10: 1-6.
3. Bradford, M. 1976. A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye-binding. Anal. Biochem. 72: 248-54.
4. Buetow, D. 1982. The Biology of Euglena, vol. III. Academic Press, New York, pp. 200-1.
5. CCAP (Culture Collection of Algae and Protozoa). 1988. Catalogue of Strains. Natural Environment Research Council, Thompson, A. S., Rhodes, J. C. and Pettman, I (Eds). Culture Collection of Algae and Protozoa, Cumbria, 19 pp.
6. Conforti, V. 1991. Taxonomic study of the Euglenophyta of a highly polluted river of Argentina. Nova Hedwigia 53: 73-98.
7. Conforti, V., Alberghina, J. and Gonzalez Urda, E. 1995. Structural change and dynamics of the phytoplankton along a highly polluted lowland river of Argentina. J. Aquat. Ecosyst. Health 4: 59-75.
8. Devars, S., Torres Marquez, M. E., Gonzalez Halphen, D., Uribe, A. and Moreno Sanchez, R. 1992. Cyanidine sensitive and cyanide resistant respiration of dark grown Euglena gracilis. Plant Sci. 82: 37-43.
9. Dubois, M., Gills, K., Hamilton, J., Rebers, P. and Smith, F. 1956. Colorimetric method of determination of sugars and related substances. Anal. Chem. 28: 350-6.
10. Einecker Lamas, M., Soares, M. J. and Oliveira, M. M. 1996. Effects of cadmium on Euglena gracilis membrane lipids. Braz. J. Med. Biol. Res. 29: 941-8.
11. Fasulo, M. P., Bassi, M. and Donini, A. 1983. Cytotoxic effects of hexavalent chromium in Euglena gracilis, II. Physiological and ultrastructural studies. Protoplasma 114: 35-43.
12. Gojdics, M. 1953. The Genus Euglena. University of Wisconsin Press, Madison, 268 pp.
13. Hosotani, K., Yokota, A., Nakano, Y. and Kitaoka, S. 1980. The metabolic pathway of propionate in Euglena gracilis Z grown under illumination. Agric. Biol. Chem. 44: 1097-103.
14. Kempner, E. S. and Miller, J. H. 1972. The molecular biology of Euglena gracilis. VII. Inorganic requirements for a minimal culture medium. J. Protozool. 19: 343-6.
15. Mackerth, F., Hiron, J. and Tailing, J. 1978. Water analysis: some revised methods for limnologists. Freshwater Biol. Assoc. Sci. Pub. 36: 117.
16. Mayer, S. and Beale, S. 1990. Light regulation of alpha-aminolevulinic acid biosynthetic enzymes and tRNA in Euglena gracilis. Plant Physiol. 94: 1365-75.
17. Nudelman, A., Rossi, M. S., Conforti, V. and Triemer, R. E. 2003. Phylogeny of Euglenophyceae based on small subunit rDNA sequences: taxonomic implications. J. Phycol. 39: 226-35.
18. Oliveira, M. M., Alves, M. F. and Brigante, A. A. 1990. Differences in phospholipid composition of autotrophic and heterotrophic Euglena gracilis. Braz. J. Med. Biol. Res. 23: 1237-41.
19. Pringsheim, E. G. 1946. The biphasic or soil-water culture method for growing algae and flagellates. J. Ecol. 33: 193-204.
20. Rocchetta, I., Ruiz, L. B., Magaz, G. and Conforti, V. 2003. Effects of hexavalent chromium in two strains of Euglena gracilis. Bull. Environ. Contam. Toxicol. 70: 1045-51.
21. Sokal, R. R. and Rohlf, F. J. 1984. Introducción a la Bioestadtstica. Serie de Biología Fundamental. Reverté, España, 362 pp.
22. Ternetz, C. 1912. Beiträge zur Morphologie und Physiologie der Euglena gracilis Klebs. Jahrb. Wiss. Bot. 51: 435.
23. U.S. Environmental Protection Agency. 1985. Short term methods for estimating the chronic toxicity of effluents and receiving waters to freshwater organisms. EPA/600/ 4-85/014/. US EPA Office of water, Washington DC, pp. 76-103.
24. Venrick, E. L. 1978. How many cells to count? In Sournia, A. (Ed.) Monographs on oceanographic methodology. Phytoplankton Manual. UNESCO Press, Paris, pp. 167-80.
25. Zumstein, H. 1900. Morphologie und Physiologie der Euglena gracilis Klebs. Jahrb. Wiss. Bot. 39: 1495.