In vitro anti-adhesive activity of an acidic polysaccharide from Panax ginseng on Porphyromonas gingivalis binding to erythrocytes

Planta Medica

Volumn 70, Issue 6, 2004, Pages 566-569

  Lee, Ji Hye ^a   Jung, Sun Lee ^b   Chung, Mi Sook ^b   Kyung, Hyun Kim ^a,c  

^a Korea University   (South Korea)

^b Duksung Women's University   (South Korea)

^c Department of Bio Nano Technology   (South Korea)

Author keywords

[No Author keywords available]

Indexed keywords

GLUCURONIC ACID; GLYCOSAMINOGLYCAN; HEPARIN; POLYGALACTURONASE; POLYSACCHARIDE; SUCRALFATE;

ARTEMIA; ARTICLE; BINDING AFFINITY; CARBOHYDRATE ANALYSIS; CORRELATION ANALYSIS; ERYTHROCYTE; GAS CHROMATOGRAPHY; GINSENG; HEMAGGLUTINATION; MICROBIAL ACTIVITY; MOLECULAR WEIGHT; NONHUMAN; PORPHYROMONAS GINGIVALIS;

ANTICOAGULANTS; CELL ADHESION; ERYTHROCYTES; HEMAGGLUTINATION; HUMANS; PANAX; PHYTOTHERAPY; PLANT EXTRACTS; PLANT LEAVES; PLANT ROOTS; POLYSACCHARIDES; PORPHYROMONAS GINGIVALIS;

ARTEMIA; ARTEMISIA; ARTEMISIA CAPILLARIS; BACTERIA (MICROORGANISMS); NEGIBACTERIA; PANAX; PANAX GINSENG; PORPHYROMONAS; PORPHYROMONAS GINGIVALIS;

EID: 3042800747     PISSN: 00320943     EISSN: None     Source Type: Journal    
DOI: 10.1055/s-2004-827160     Document Type: Article

References (16)

1. Haffajee AD, Socransky SS. Microbial etiological agents of destructive periodontal diseases. Periodontology 2000; 5: 78-111
2. Nakayama K, Ratnayake DB, Tsukuba T, Kadowaki T, Yamamoto K, Fujimura S. Hemoglobin receptor protein is intragenially encoded by the cysteine proteinase-encoding genes and the hemagglutinin-encoding gene of Porphyromonas gingivalis. Mol Microbiol 1998; 27: 51-61
3. Potempa J, Pike R, Travis J. The multiple forms of trypsin-like activity present in various strains of Porphyromonas gingivalis are due to the presence of either Arg-gingipain or Lys-gingipain. Infect Immun 1995; 63: 1176-82
4. Shi Y, Ratnayake DB, Okamoto K, Abe N, Yamamoto K, Nakayama K. Genetic analyses of proteolysis, hemoglobin binding, and hemagglutination of Porphyromonas gingivalis. J Biol Chem 1999; 274: 17955-60
5. Kontani M, Ono H, Shibata H, Okamura Y, Tanaka T, Fujiwara T, Kimura S, Hamada S. Cysteine protease of Porphyromonas gingivalis 381 enhances binding of fimbriae to cultured human fibroblasts and matrix proteins. Infect Immun 1996; 64: 756-62
6. Nakamura T, Amano A, Nakagawa I, Hamada S. Specific interactions between Porphyromonas gingivalis fimbrae and human extracellular matrix proteins. FEMS Microbiol Lett 1999; 175: 267-72
7. Sojar HT, Sharma A, Genco RJ. Porphyromonas gingivalis fimbrae bind to cytokeratin of epithelial cells. Infect Immun 2002; 70: 96-101
8. Lamont RJ, Jenkinson HF. Subgingival colonization by Porphyromonas gingivalis. Oral Microbiol Immunol 2000; 15: 341-9
9. Wadstrom T, Ljungh A. Glycosaminoglycan-binding microbial proteins in tissue adhesion and invasion: key events in microbial pathogenicity. J Med Microbiol 1999; 48: 223-33
10. Agnani G, Tricot-Doleux S, Houalet S, Bonnaure-Mallet M. Epithelial cell surface sites involved in the polyvalent adherence of Porphyromonas gingivalis: a convincing role for neuraminic acid and glucuronic acid. Infect Immun 2003; 71: 991-6
11. Belogortseva NI, Yoon JY, Kim KH. Inhibition of Helicobacter pylori hemagglutination by polysaccharide fractions from Roots of Panax ginseng. Planta Med 2000; 66: 217-20
12. Woo JS, Ha BH, Kim TG, Lim Y, Kim KH. Development of an enzyme-linked glycosorbent method to monitor the inhibition of sialic acid-dependent Helicobacter pylori adhesion. Biotechnol Lett 2001; 23: 507-11
13. Lee JH, Park EK, Uhm CS, Chung MS, Kim KH. Inhibition of Helicobacter pylori adhesion to human gastric adenocarcinoma epithelial cells by acidic polysaccharides from Artemisia capillaris and Panax ginseng. Planta Med 2004; 70: 1-4
14. Tomoda M, Hirabayashi K, Shimizu N, Gonda R, Ohara N. The core structure of ginsenan PA, a phagocytosis-activating polysaccharide from the root of Panax ginseng. Biol Pharm Bull 1994; 17: 1287-91
15. Chaplin MF. Monosaccharides, 1. Carbohydrate analysis. In: Chaplin MF, Kennedy JF, editors. Oxford: IRL Press, 1986: pp 1-36
16. Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Chem 1976; 72: 248-54