HIV-1 integrase complexes with DNA dissociate in the presence of short oligonucleotides conjugated to acridine

Biochemistry

Volumn 43, Issue 27, 2004, Pages 8735-8743

  Pinskaya, Marina ^a   Romanova, Elena ^a   Volkov, Evgenii ^a   Deprez, Eric ^c   Leh, Hervé ^b   Brochon, Jean Claude ^c   Mouscadet, Jean François ^c   Gottikh, Marina ^a  

^a MOSCOW STATE UNIVERSITY   (Russian Federation)

^b BioAlliance Pharma   (France)

^c Ecole Normale Superieure de Cachan   (France)

Author keywords

[No Author keywords available]

Indexed keywords

COMPLEXATION; DISSOCIATION; DNA; DRUG DOSAGE; ENZYME KINETICS; IMMUNOLOGY; OLIGOMERS;

ANTIVIRAL DRUGS; BINDING SITES;

BIOCHEMISTRY;

ACRIDINE DERIVATIVE; DNA; INTEGRASE; OLIGONUCLEOTIDE; VIRUS ENZYME;

ANISOTROPY; ARTICLE; BINDING SITE; DNA PROTEIN COMPLEX; ENZYME SUBSTRATE COMPLEX; FLUORESCENCE; GENE EXPRESSION; HUMAN IMMUNODEFICIENCY VIRUS 1; HYDROPHOBICITY; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; REVERSED PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; STEADY STATE; VIRUS REPLICATION;

ACRIDINES; BASE SEQUENCE; BINDING SITES; DNA; HEPARIN; HIV INTEGRASE; HIV INTEGRASE INHIBITORS; INHIBITORY CONCENTRATION 50; OLIGONUCLEOTIDES; PROTEIN BINDING;

HUMAN IMMUNODEFICIENCY VIRUS; HUMAN IMMUNODEFICIENCY VIRUS 1; RNA VIRUSES;

EID: 3042772950     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi049706m     Document Type: Article

References (43)

1. Palella, F. J. J., Delaney, K. M., Moorman, A. C., Loveless, M. O., Fuhrer, J., Satten, G. A., Aschman, D. J., and Holmberg, S. D. (1998) Declining morbidity and mortality among patients with advanced human immunodeficiency virus infection, N. Engl. J. Med. 338, 853-860.
2. Ramratnam, B., Mittler, J. E., Zhang, L., Boden, D., Hurley, A., Fang, F., Macken, C. A., Perelson, A. S., Markowitz, M., and Ho, D. D. (2000) The decay of the latent reservoir of replication-competent HIV-1 is inversely correlated with the extent of residual viral replication during prolonged anti-retroviral therapy, Nat. Med. 6, 82-85.
3. Finzi, D., Blankson, J., Siliciano, J. D., Margolick, J. B., Chadwick, K., Pierson, T., Smith, K., Lisziewicz, J., Lori, F., Flexner, C., Quinn, T. C., Chaisson, R. E., Rosenberg, E., Walker, B., Gange, S., Gallant, J., and Siliciano, R. F. (1999) Latent infection of CD4+ T cells provides a mechanism for lifelong persistence of HIV-1, even in patients on effective combination therapy, Nat. Med. 5, 512-517.
4. Bukrinsky, M. I., Sharova, N., Dempsey, M. P., Stanwick, T. L., Bukrinskaya, A. G., Haggerty, S., and Stevenson, M. (1992) Active nuclear import of human immunodeficiency virus type 1 preintegration complexes, Proc. Natl. Acad. Sci. U.S.A. 89, 6580-6584.
5. Gao, K., Butler, S. L., and Bushman, F. (2001) Human immunodeficiency virus type 1 integrase: arrangement of protein domains in active cDNA complexes, EMBO J. 20, 3565-3576.
6. Perryman, A. L. and McCammon, J. A. (2002) AutoDocking Dinucleotides to the HIV-1 Integrase Core Domain: Exploring Possible Binding Sites for Viral and Genomic DNA, J. Med. Chem. 45, 5624-5627.
7. Mazumder, A., Neamati, N., Pilon, A. A., Sunder, S., and Pommier, Y. (1996) Chemical trapping of ternary complexes of human immunodeficiency virus type 1 integrase, divalent metal, and DNA substrates containing an abasic site. Implications for the role of lysine 136 in DNA binding, J. Biol. Chem. 271, 27330-27338.
8. Drake, R. R., Neamati, N., Hong, H., Pilon, A. A., Sunthankar, P., Hume, S. D., Milne, G. W., and Pommier, Y. (1998) Identification of a nucleotide binding site in HIV-1 integrase, Proc. Natl. Acad. Sci. U.S.A. 95, 4170-4175.
9. Jenkins, T. M., Esposito, D., Engelman, A., and Craigie, R. (1997) Critical contacts between HIV-1 integrase and viral DNA identified by structure-based analysis and photocrosslinking, EMBO J. 16, 6849-6859.
10. Podtelezhnikov, A. A., Gao, K., Bushman, F. D., and McCammon, J. A. (2003) Modeling HIV-1 integrase complexes based on their hydrodynamic properties, Biopolymers 68, 110-120.
11. Taktakishvili, M., Neamati, N., Pommier, Y., and Nair, V. (2001) Discovery of a nuclease-resistant, nonnatural dinucleotide that inhibits HIV-1 integrase, Bioorg. Med. Chem. Lett. 11, 1433-1435.
12. Mazumder, A., Cooney, D., Agbaria, R., Gupta, M., and Pommier, Y. (1994) Inhibition of human immunodeficiency virus type 1 integrase by 3′-azido-3′-deoxythymidylate, Proc. Natl. Acad. Sci. U.S.A. 91, 5771-5775.
13. Caumont, A., Jamieson, G., de, S. V., Parissi, V., Foumier, M., Zakharova, O. D., Bayandin, R., Litvak, S., Tarrago-Litvak, L., and Nevinsky, G. A. (1999) High affinity interaction of HIV-1 integrase with specific and nonspecific single-stranded short oligonucleotides, FEBS Lett. 455, 154-158.
14. Tramontano, E., Colla, P. L., and Cheng, Y. C. (1998) Biochemical characterization of the HIV-1 integrase 3′-processing activity and its inhibition by phosphorothioate oligonucleotides, Biochemistry 37, 7237-7243.
15. Mazumder, A., Neamati, N., Ojwang, J. O., Sunder, S., Rando, R. F., and Pommier, Y. (1996) Inhibition of the human immunodeficiency virus type 1 integrase by guanosine quartet structures, Biochemistry 35, 13762-13771.
16. Brodin, P., Pinskaya, M., Volkov, E., Romanova, E., Leh, H., Auclair, C., Mouscadet, J. F., and Gottikh, M. (1999) Branched oligonucleotide-intercalator conjugate forming a parallel stranded structure inhibits HIV-1 integrase, FEBS Lett. 460, 270-274.
17. Cherepanov, P., Este, J. A., Rando, R. F., Ojwang, J. O., Reekmans, G., Steinfeld, R., David, G., De Clercq, E., and Debyser, Z. (1997) Mode of interaction of G-quartets with the integrase of human immunodeficiency virus type 1, Mol. Pharmacol. 52, 771-780.
18. Mouscadet, J. F., Ketterle, C., Goulaouic, H., Carteau, S., Subra, F., Le, B. M., and Auclair, C. (1994) Triple helix formation with short oligonucleotide-intercalator conjugates matching the HIV-1 U3 LTR end sequence, Biochemistry 33, 4187-4196.
19. Mouscadet, J. F., Carteau, S., Goulaouic, H., Subra, F., and Auclair, C. (1994) Triplex-mediated inhibition of HIV DNA integration in vitro, J. Biol. Chem. 269, 21635-21638.
20. Bouziane, M., Cherny, D. I., Mouscadet, J. F., and Auclair, C. (1996) Alternate strand DNA triple helix-mediated inhibition of HIV-1 U5 long terminal repeat integration in vitro, J. Biol. Chem. 271, 10359-10364.
21. Este, J. A., Cabrera, C., Schols, D., Cherepanov, P., Gutierrez, A., Witvrouw, M., Pannecouque, C., Debyser, Z., Rando, R. F., Clotet, B., Desmyter, J., and De Clercq, E. (1998) Human immunodeficiency virus glycoprotein gp120 as the primary target for the antiviral action of AR177 (Zintevir), Mol. Pharmacol. 53, 340-345.
22. Pemberton, I. K., Buckle, M., and Buc, H. (1996) The metal ion-induced cooperative binding of HIV-1 integrase to DNA exhibits a marked preference for Mn(II) rather than Mg(II), J. Biol. Chem. 271, 1498-1506.
23. Wai, J. S., Egbertson, M. S., Payne, L. S., Fisher, T. E., Embrey, M. W., Tran, L. O., Melamed, J. Y., Langford, H. M., Guare, J. P., Zhuang, L., Grey, V. E., Vacca, J. P., Holloway, M. K., Naylor-Olsen, A. M., Hazuda, D. J., Felock, P. J., Wolfe, A. L., Stillmock, K. A., Schleif, W. A., Gabryelski, L. J., and Young, S. D. (2000) 4-Aryl-2,4-dioxobutanoic acid inhibitors of HIV-1 integrase and viral replication in cells, J. Med. Chem. 43, 4923-4926.
24. Espeseth, A. S., Felock, P., Wolfe, A., Witmer, M., Grobler, J., Anthony, N., Egbertson, M., Melamed, J. Y., Young, S., Hamill, T., Cole, J. L., and Hazuda, D. J. (2000) HIV-1 integrase inhibitors that compete with the target DNA substrate define a unique strand transfer conformation for integrase, Proc. Natl. Acad. Sci. U.S.A. 97, 11244-11249.
25. Hazuda, D. J., Felock, P., Witmer, M., Wolfe, A., Stillmock, K., Grobler, J. A., Espeseth, A., Gabryelski, L., Schleif, W., Blau, C., and Miller, M. D. (2000) Inhibitors of strand transfer that prevent integration and inhibit HIV-1 replication in cells, Science 287, 646-650.
26. Brodin, P., Pinskaya, M., Buckle, M., Parsch, U., Romanova, E., Engels, J., Gottikh, M., and Mouscadet, J. F. (2002) Disruption of HIV-1 Integrase-DNA Complexes by Short 6-Oxocytosine-Containing Oligonucleotides, Biochemistry 41, 1529-1538.
27. Brodin, P., Pinskaya, M., Parsch, U., Bischerour, J., Leh, H., Romanova, E., Engels, J. W., Gottikh, M., and Mouscadet, J. F. (2001) 6-oxocytidine containing oligonucleotides inhibit the HIV-1 integrase in vitro, Nucleosides Nucleotides Nucleic Acids 20, 481-486.
28. Goldgur, Y., Craigie, R., Cohen, G. H., Fujiwara, T., Yoshinaga, T., Fujishita, T., Sugimoto, H., Endo, T., Murai, H., and Davies, D. R. (1999) Structure of the HIV-1 integrase catalytic domain complexed with an inhibitor: a platform for antiviral drug design, Proc. Natl. Acad. Sci. U.S.A. 96, 13040-13043.
29. Pommier, Y., Marchand, C., and Neamati, N. (2000) Retroviral integrase inhibitors year 2000: update and perspectives, Antiviral Res. 47, 139-148.
30. Bischerour, J., Leh, H., Deprez, E., Brochon, J. C., and Mouscadet, J. F. (2003) Disulfide-linked integrase oligomers involving c280 residues are formed in vitro and in vivo but are not essential for human immunodeficiency virus replication, J. Virol. 77, 135-141.
31. Brodin, P., Gottikh, M., Auclair, C., and Mouscadet, J. F. (1999) Inhibition of HIV-1 integration by mono- & bifunctionalized triple helix forming oligonucleotides, Nucleosides Nucleotides 18, 1717-1718.
32. Heuer, T. S. and Brown, P. O. (1998) Photocross-linking studies suggest a model for the architecture of an active human immunodeficiency virus type 1 integrase-DNA complex, Biochemistry 37, 6667-6678.
33. Brown, M. P. and Royer, C. (1997) Fluorescence spectroscopy as a tool to investigate protein interactions, Curr. Opin. Biotechnol. 8, 45-49.
34. Alexander, K. A. and Phelps, W. C. (1996) A fluorescence anisotropy study of DNA binding by HPV-11 E2C protein: a hierarchy of E2-binding sites, Biochemistry 35, 9864-9872.
35. Deprez, E., Barbe, S., Kolaski, M., Leh, H., Zouhiri, F., Auclair, C., Brochon, J. C., Le Bret, M., and Mouscadet, J. F. (2004) Mechanism of HIV-1 Integrase Inhibition by Styrylquinoline Derivatives in Vitro, Mol. Pharmacol. 65, 85-98.
36. Deprez, E., Tauc, P., Leh, H., Mouscadet, J. F., Auclair, C., Hawkins, M. E., and Brochon, J. C. (2001) DNA binding induces dissociation of the multimeric form of HIV-1 integrase: a time-resolved fluorescence anisotropy study, Proc. Natl. Acad. Sci. U.S.A. 98, 10090-10095.
37. Walker, S. J., Pizzato, M., Takeuchi, Y., and Devereux, S. (2002) Heparin binds to murine leukemia virus and inhibits Env-independent attachment and infection, J. Virol. 76, 6909-6918.
38. Carteau, S., Mouscadet, J. F., Goulaouic, H., Subra, F., and Auclair, C. (1993) Inhibitory effect of the polyanionic drug suramin on the in vitro HIV DNA integration reaction, Arch. Biochem. Biophys. 305, 606-610.
39. Rice, P. A. and Baker, T. A. (2001) Comparative architecture of transposase and integrase complexes, Nat. Struct. Biol. 8, 302-307.
40. Pemberton, I. K., Buc, H., and Buckle, M. (1998) Displacement of viral DNA termini from stable HIV-1 integrase nucleoprotein complexes induced by secondary DNA-binding interactions, Biochemistry 37, 2682-2690.
41. Tortora, G. and Ciardiello, F. (2003) Antisense Targeting Protein Kinase A Type I as a Drug for Integrated Strategies of Cancer Therapy, Ann. N. Y. Acad. Sci. 1002, 236-243.
42. Kurreck, J. (2003) Antisense technologies. Improvement through novel chemical modifications, Eur. J. Biochem. 270, 1628-1644.
43. Shuey, D. J., McCallus, D. E., and Giordano, T. (2002) RNAi: gene-silencing in therapeutic intervention, Drug Discov. Today 7, 1040-1046.