Immunohistochemistry of matrix markers in Technovit 9100 New®-embedded undecalcified bone sections

European Cells and Materials

Volumn 6, Issue , 2003, Pages 57-71

  Yang, R ^a   Davies, C M ^a   Archer, C W ^b   Richards, R G ^a   Plenk, H ^c   Rooney, P ^c   Gasser, J ^c  

^a AO RESEARCH INSTITUTE   (Switzerland)

^b CARDIFF UNIVERSITY   (United Kingdom)

^c NONE

Author keywords

Acrylate embedding; Alkaline phosphatase; Bone sialoprotein; Histomorphology; Immunohistochemistry; Osteocalcin; Osteopontin; Procollagen type I; Technovit 9100 New ; Undecalcified cancellous bone

Indexed keywords

ACRYLIC ACID RESIN; ALKALINE PHOSPHATASE; ANTIBODY; BIOLOGICAL MARKER; METHACRYLIC ACID METHYL ESTER; OSTEOCALCIN; OSTEONECTIN; OSTEOPONTIN; PEPTIDE; PROCOLLAGEN TYPE 1 AMINOPROPEPTIDE; SIALOPROTEIN; UNCLASSIFIED DRUG; LR WHITE; PEPTIDE FRAGMENT; POLY(METHYL METHACRYLATE); PROCOLLAGEN; PROCOLLAGEN TYPE I N TERMINAL PEPTIDE; PROCOLLAGEN TYPE I N-TERMINAL PEPTIDE; SIALOGLYCOPROTEIN; SPP1 PROTEIN, HUMAN;

AGED; ANIMAL TISSUE; ANTIBODY LABELING; ARTICLE; BONE MATRIX; CONTROLLED STUDY; EMBEDDING; FEMALE; HISTOLOGY; HUMAN; HUMAN TISSUE; IMMUNOHISTOCHEMISTRY; INTERMETHOD COMPARISON; LOW TEMPERATURE PROCEDURES; MALE; MORPHOLOGY; NONHUMAN; STAINING; TRABECULAR BONE; ANIMAL; BONE; CATTLE; CHEMISTRY; COMPARATIVE STUDY; METHODOLOGY; OSTEOBLAST; OSTEOCLAST; SENSITIVITY AND SPECIFICITY; SHEEP;

ACRYLIC RESINS; AGED; ALKALINE PHOSPHATASE; ANIMALS; BIOLOGICAL MARKERS; BONE AND BONES; BONE MATRIX; CATTLE; DECALCIFICATION TECHNIQUE; FEMALE; HUMANS; IMMUNOHISTOCHEMISTRY; MALE; METHYLMETHACRYLATE; OSTEOBLASTS; OSTEOCALCIN; OSTEOCLASTS; OSTEONECTIN; OSTEOPONTIN; PEPTIDE FRAGMENTS; POLYMETHYL METHACRYLATE; PROCOLLAGEN; SENSITIVITY AND SPECIFICITY; SHEEP; SIALOGLYCOPROTEINS; TISSUE EMBEDDING;

EID: 3042747950     PISSN: 14732262     EISSN: None     Source Type: Journal    
DOI: 10.22203/eCM.v006a06     Document Type: Article

References (54)

1. Arana-Chavez VE, Nanci A (2001) High-resolution immunocytochemistry of noncollagenous matrix proteins in rat mandibles processed with microwave irradiation. J Histochem Cytochem 49:1099-1109.
2. Arai N, Ohya K, Ogura H (1993) Osteopontin mRNA expression during bone resorption: an in situ hybridization study of induced ectopic bone in the rat. Bone Miner 22: 129-145.
3. Arnold WH, Gaengler P, Kalkutschke L (1998) Three-dimensional reconstruction of approximal subsurface caries lesions in deciduous molars. Clin Oral Investig 2: 174-179.
4. Arnold WH, Konopka S, Gaengler P (2001) Qualitative and quantitative assessment of intratubular dentin formation in human natural carious lesions. Calcif Tissue Int 69: 268-273.
5. Arnold WH, Konopka S, Kriwalsky MS, Gaengler P (2003) Morphological analysis and chemical content of natural dentin carious lesion zones. Ann Anat 185: 419-424.
6. Bernhards J, Weitzel B, Werner M, Rimpler M, Georgii A (1992). A new histological embedding method by low-temperature polymerisation of methyl methacrylate allowing immuno- and enzyme histochemical studies on semi-thin sections of undecalcified bone marrow biopsies. Histochem 98: 145-154.
7. Bianco P, Silvestrini G, Termine J, Bonucci E (1988) Immunohistochemical localization of osteonectin in developing human and calf bone using monoclonal antibodies. Calcif Tissue Int 43: 155-161.
8. Bhattacharyya TK, Sanchez B, Toriumi D (2000) Evaluation of LR white resin for histology of the undecalcified rat tibia. Biotech Histochem 75: 7-14.
9. Bolander ME, Gehron-Robez P, Fisher LW, Conn KM, Prabhakar BS, Termine JD (1989) Monoclonal antibodies against osteonectin show conservation of epitopes across species. Calcif Tissue Int 45: 74-80.
10. Bord S, Vedi S, Beavan SR, Horner A, Compston JE (2000) Megakaryocyte population in human bone marrow increases with estrogen treatment: a role in bone remodeling? Bone 27: 397-401.
11. Bronckers A, Gay S, Finkelman R, Butler W (1987) Developmental appearance of G1a proteins (osteocalcin) and alkaline phosphatase in tooth germs and bones of the rat. J Bone Miner Res 2: 361-373.
12. Bronckers A, Farach-Carson M., van Waveren E, Butler W (1994) Immunolocalization of osteopontin, osteocalcin, and dentin sialoprotein during dental root formation and early cementogenesis in the rat. J Bone Miner Res 9: 833-841.
13. Brorson SH, Roos N, Skjorten F (1994) Antibody penetration into LR-White sections. Micron 25: 453-460.
14. Burkhardt R (1966) Preparatory conditions for clinical histology of human bone marrow. 2. A new procedure for histological preparation of bone and bone marrow biopsies (in German). Blut 14: 30-46.
15. Chappard D, Alexandre C, Camps M, Montheard JP, Riffat G (1983) Embedding iliac bone biopsies at low temperature using glycol and methyl methacrylates. Stain Technol 58: 299-308.
16. Chappard, D, Palle, S, Alexandre, C, Vico, L, Riffat, G (1987) Bone embedding in pure methyl methacrylate at low temperature preserves enzyme activities. Acta histochem 81:183-190.
17. Erben RG (1997) Embedding of bone samples in methyl methacrylate: an improved method suitable for bone histomorphometry, histochemistry, and immunohistochemistry. J Histochem Cytochem 45: 307-313.
18. Fisher LW, Stubbs JT, Young MF (1995) Antisera and cDNA probes to human and certain animal model bone matrix noncollagenous proteins. Acta Orthop Scand (Suppl 266) 66: 61-65.
19. Foellmer HG, Kawahara K, Madri JA, Furthmayr H, Timpl R, Tuderman L (1983) A monoclonal antibody specific for the amino terminal cleavage site of procollagen type I. Eur J Biochem 134: 183-189.
20. Georgii A, Bernhards J, Werner M (1995). Die neue Hannover-methode zur kunststoffeinbettung von knochenmark: kaltpolymerisation von methylmethacrlyat (The new Hannover method for plastic embedding of bone marrow: cold polymerization of methylmethacrylate). Pathologe 16: 28-33.
21. Hauge EM, Qvesel D, Eriksen EF, Mosekilde L, Melsen F (2001). Cancellous bone remodeling occurs in specialized compartments lined by cells expressing osteoblastic markers. J Bone Mineral Res 16:1575-1582.
22. Hughes FJ, Aubin JE (1998) Culture of cells of the osteoblast lineage. In: Methods in Bone Biology. Arnett TRHB (ed.). Chapman and Hall, London. pp 1-39.
23. Ingram RT, Clarke BL, Fisher LW, Fitzpatrick LA (1993) Distribution of noncollagenous proteins in the matrix of adult human bone: evidence of anatomic and functional heterogeneity. J Bone Min Res 8:1019-1029.
24. Jundt G, Berghaeuser K, Termine J, Schulz A (1987) Osteonectin - a differentiation marker of bone cells. Cell Tissue Res 248: 409-415.
25. Kato Y, Windle JJ, Koop BA, Mundy GR, Bonewald LF (1997). Establishment of an Osteocyte-like cell line, MLO-Y4. J Bone Mineral Res 12: 2014-2023.
26. Lawson GM, Katzman JA, Kimlinger TK, O'Brien JF (1985) Isolation and preliminary characterization of a monoclonal antibody that interacts preferentially with the liver isoenzyme of human alkaline phosphatase. Clin Chem 31: 381-385.
27. Lebeau A, Muthmann H, Sendelhofert A, Diebold J, Löhrs U (1995) Histochemistry and immunohistochemistry on bone marrow biopsies: a rapid procedure for methyl methacrylate embedding. Path Res Pract 191:121-129.
28. Liu C (1987). A simplified technique for low temperature methyl methacrylate embedding. Stain Technol 62:155-159.
29. Lucena SB, Duarte MEL, Fonseca EC (1997). Plastic embedded undecalcified bone biopsies: an immunohistochemical method for routine study of bone marrow extracellular matrix. J Histotechnol 20: 253-257.
30. Miao D, Bai X, Panda D, McKee M, Karaplis A, Goltzman D (2001) Osteomalacia in hyp mice is associated with abnormal phex expression and with altered bone matrix protein expression and deposition. Endocrinology 142: 926-939.
31. Mikuni-Takagaki Y, Kakai Y, Satohoshi M, Kawano E, Suzuki Y, Kawase T, Saito S (1995). Matrix mineralisation and the differentiation of osteocyte-like cells in culture. J Bone Miner Res 10: 231-242.
32. Newman GR, Jasani B, Williams ED (1982). The preservation of ultrastructure and antigenicity. J Microsc 127: RP5-RP6.
33. Newman GR, Hobot JA (2001) Resin Microscopy and On-Section Immunocytochemistry, 2nd ed., Springer-Verlag, Berlin Heidelberg New York.
34. Park YK, Yang MH, Park HR (1996) The impact of osteonectin for differential diagnosis of osteogenic bone tumors: an immunohistochemical and in situ hybridization approach. Skeletal Radiol 25: 13-17.
35. Parker CR, Freemont AJ, Blackwell PJ, Grainge MJ, Hosking DJ (1999) Cross-sectional analysis of renal transplantation osteoporosis. J Bone Miner Res 14: 1943-1951.
36. Rauch F, Lauzier D, Croteau S, Travers R, Glorieux FH, Hamdy R (2000) Temporal and spatial expression of bone morphogenetic protein-2, -4, and -7 during distraction osteogenesis in rabbits. Bone 27: 453-459.
37. Richards RG, Stiffanic M, Owen GRh, Riehle M, ap Gwynn I, Curtis ASG (2001) Immunogold labelling of fibroblast focal adhesion sites visualised in fixed material using scanning electron microscopy and in vivo, using Internal Reflection Microscopy. Cell Biol Int 25: 1237-1249.
38. Robey PM (1996). Bone matrix proteoglycans and glycoproteins. In Principles of Bone Biology. Bilezikian JP, Raisz LG, Rodan GA (eds.). Academic Press, London. pp 155-165.
39. Ruddell CL (1967) Hydroxyethyl methacrylate combined with polyethylene glycol 400 and water; and embedding medium for routine 1-2 micron sectioning. Stain Technol 42: 119-123.
40. Saito C, Hayashi M, Sakai A, Fujie M, Kuroiwa H, Kuroiwa T (1998) Improved Sensitivity for high resolution in situ hybridization using resin extraction of methyl methacrylate embedded material. Biotechnic Histochem 74: 40-48.
41. Sanderson C (1997) Entering the realm of mineralized bone processing: a review of the literature and techniques. J Histotechnol 20: 259-266.
42. Schenk R (1965) On the histological processing of undecalcified bone. Acta Anat (Basel), 60: 3-19.
43. Semba I, Ishigami T, Sugihara K, Kitano M. (2000) Higher osteoclastic demineralization and highly mineralized cement lines with osteocalcin deposition in a mandibular cortical bone of autosomal dominant osteopetrosis type II: ultrastructural and undecalcified histological investigations. Bone 27: 389-395.
44. Stafford H, Roberts M, Oni O, Hay J, Gregg P (1994) Localisation of bone-forming cells during fracture healing by osteocalcin immunocytochemistry: An experimental study of the rabbit tibia. J Orthop Res 12: 29-39.
45. Strehblow C, Gyongyosi M, Sperker W, Shirazi M, Windberger U, Pugatsch T, Ben-Sasson S, Lotan C, Glogar D (2002) Usefulness of intravascular ultrasound-guided histological measurements after stenting in porcine coronary artery. Coron Artery Dis 13: 291-294.
46. Te Velde J, Haak HL (1977) Aplastic anaemia. Histological investigation of methacrylate embedded bone marrow biopsy specimens; correlation with survival after conventional treatment in 15 adult patients. Br J Haematol, 35: 61-69.
47. Technovit 9100 New® Product Brochure, Heraeus Kulzer GmbH Co. KG, ++49-6081-959-0, http://www.Kulzer-Technik.de. 2001. (Pamphlet).
48. Thomsen JS, Ebbesen EN, Mosekilde L (1998) Relationships between static histomorphometry and bone strength measurements in human iliac crest bone biopsies. Bone 22: 153-163.
49. Vedi S, Shaun G, Skingle SJ, Garrahan NJ, Ninkovic M, Alexander GA, Compston JE (1999) Mechanism of bone loss after liver transplantation: a histomorphometric analysis. J Bone Min Res 14: 281-287.
50. Westen H, Muck KF, Post L (1981) Enzyme histochemistry on bone marrow sections after embedding in methacrylate at low temperature. Histochemistry, 70: 95-105.
51. Wolf E, Röser K, Han M, Welkerling H, Delling G (1992). Enzyme and immunohistochemistry on undecalcified bone and bone marrow biopsies after embedding in plastic: a new embedding method for routine application. Virchows Arch A 420: 17-24.
52. Yang. R (2003) Evaluation of Technovit 9100 New and LR White resins for Undecalcified Trabecular Bone Histology and Immunohistochemistry. M.Phil. Dissertation. University of Wales.
53. Yoshimura N, Murachi T, Heath R, Kay J, Jasani B, Newman GR (1986). Immunogold electron microscopic localization of calpain I in skeletal muscle of rats. Cell Tissue Res 244: 265-270.
54. Müller R. (2003) Immunological identification of the osteoclast formation regulator osteoprotegerin ligand (OPGL/RANKL/TRANCE). M.Sc. Dissertation. University Medical Centre, Nijmegen, Netherlands