Mass spectrometric imaging of highly curved membranes during Tetrahymena mating

Science

Volumn 305, Issue 5680, 2004, Pages 71-73

  Ostrowski, Sara G ^a   Van Bell, Craig T ^b   Winograd, Nicholas ^a   Ewing, Andrew G ^a  

^a PENNSYLVANIA STATE UNIVERSITY   (United States)

^b EDINBORO UNIVERSITY OF PENNSYLVANIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

LIPIDS; MASS SPECTROMETRY; PRINCIPAL COMPONENT ANALYSIS;

BIOLOGICAL MEMBRANE FUSION; FUSION SITES;

BIOLOGICAL MEMBRANES;

2 AMINOETHYLPHOSPHONOLIPID; LIPID; PHOSPHATIDYLCHOLINE; UNCLASSIFIED DRUG;

BIOLOGY;

ARTICLE; CHANNEL GATING; EXOCYTOSIS; IMAGING; LIPID BILAYER; MASS SPECTROMETRY; MATING; MEMBRANE FUSION; NONHUMAN; PRINCIPAL COMPONENT ANALYSIS; PRIORITY JOURNAL; REPRODUCTION; TETRAHYMENA;

ANIMALS; CELL MEMBRANE; FREEZE FRACTURING; LIPID BILAYERS; MEMBRANE FUSION; MEMBRANE LIPIDS; PHOSPHATIDYLCHOLINES; PHOSPHOLIPIDS; PRINCIPAL COMPONENT ANALYSIS; SPECTROMETRY, MASS, SECONDARY ION; TETRAHYMENA THERMOPHILA;

PROTOZOA; TETRAHYMENA;

EID: 3042706949     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.1099791     Document Type: Article

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28. Supported in part by a grant from NIH and additional instrumentation support provided by NSF. We thank M. Wagner for sharing his expertise in multivariate statistics and applying principal component analysis to the SIMS data, T. Roddy for helpful discussions and suggestions, M. Hazen and the Pennsylvania State University Electron Microscopy facility for assistance with the TEM images, and P. Powell and L. Sombers for help with peak fitting and statistical analysis of the data.