Single nucleotide polymorphism genotyping of the barley waxy gene by polymerase chain reaction with confronting two-pair primers

Plant Breeding

Volumn 123, Issue 3, 2004, Pages 225-228

  Domon, E ^a   Yanagisawa, T ^b   Saito, A ^a   Takeda, K ^c  

^a NATIONAL AGRICULTURAL RESEARCH CENTER FOR KYUSHU OKINAWA REGION   (Japan)

^b NATIONAL AGRICULTURAL RESEARCH CENTER FOR WESTERN REGION   (Japan)

^c OKAYAMA UNIVERSITY   (Japan)

Author keywords

Beta glucan; dCAPS; DNA marker; Hordeum vulgare; Marker assisted selection; PCR CTPP; SNP; Waxy gene

Indexed keywords

HORDEUM; HORDEUM VULGARE; HORDEUM VULGARE SUBSP. VULGARE;

EID: 3042627511     PISSN: 01799541     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1439-0523.2004.00970.x     Document Type: Article

References (14)

1. Briard, M., M. Le Clerc, D. Grzebelus, D. Senalik, and P. W. Simon, 2000: Modified protocols for rapid carrot genomic DNA extraction and AFLP analysis using silver staining or radioisotopes. Plant Mol. Biol. Rep. 18, 235-241.
2. Domon, E., A. Saito, and K. Takeda, 2002: Comparison of the waxy locus sequence from a non-waxy strain and two waxy mutants of spontaneous and artificial origins in barley. Genes Genet. Syst. 77, 351-359.
3. Hamajima, N., T. Saito, K. Matsuo, K. Kozaki, T. Takahashi, and K. Tajima, 2000: Polymerase chain reaction with confronting two-pair primers for polymorphism genotyping. Jpn. J. Cancer Res. 91, 865-868.
4. Hamajima, N., T. Saito, K. Matsuo, and K. Tajima, 2002: Competitive amplification and unspecific amplification in polymerase chain reaction with confronting two-pair primers. J. Mol. Diagn. 4, 103-107.
5. Heid, C. A., J. Stevens, K. J. Livak, and P. M. Williams, 1996: Real time quantitative PCR. Genome Res. 6, 986-994.
6. Ishikawa, N., J. Ishihara, and M. Itoh, 1995: Artificial induction and characterization of amylose-free mutants of barley. Barley Genet. Newsl. 24, 49-53.
7. Izydorczyk, M. S., J. Storsley, D. Labossiere, A. W. MacGregor, and B. G. Rossnagel, 2000: Variation in total and soluble beta-glucan content in hulless barley: effects of thermal, physical, and enzymic treatments. J. Agric. Food Chem. 48, 982-989.
8. Jenkins, D. J., C. W. Kendall, V. Vuksan, E. Vidgen, T. Parker, D. Faulkner, C. C. Mehling, M. Garsetti, G. Testolin, S. C. Cunnane, M. A. Ryan, and P. N. Corey, 2002: Soluble fiber intake at a dose approved by the US Food and Drug Administration for a claim of health benefits: serum lipid risk factors for cardiovascular disease assessed in a randomized controlled crossover trial. Am. J. Clin. Nutr. 75, 834-839.
9. Kokoris, M., K. Dix, K. Moynihan, J. Mathis, B. Erwin, P. Grass, B. Mines, and A. Duesterhoeft, 2000: High-throughput SNP genotyping with the Masscode system. Mol. Diagn. 5, 329-340.
10. Neff, M. M., J. D. Neff, J. Chory, and A. E. Pepper, 1998: dCAPS, a simple technique for the genetic analysis of single nucleotide polymorphisms: experimental applications in Arabidopsis thaliana genetics. Plant J. 14, 387-392.
11. Rohde, W., Becker, D., and Salamini, F., 1988: Structural analysis of the waxy locus from Hordeum vulgare. Nucleic Acids Res. 16, 7185-7186.
12. Waterfall, C. M., and B. D. Cobb, 2002: SNP genotyping using single-tube fluorescent bidirectional PCR. Biotechniques 33, 80-86.
13. Yasui, T., M. Seguchi, N. Ishikawa, and M. Fujita, 2002: Starch properties of a waxy mutant line of hull-less barley (Hordeum vulgare L.). Starch-Starke 54, 179-184.
14. Ye, S., S. Dhillon, X. Ke, A. R. Collins, and I. N. Day, 2001: An efficient procedure for genotyping single nucleotide polymorphisms. Nucleic Acids Res. 29, 88-96.